MiR-125a-3p-KLF15-BCAA Regulates the Skeletal Muscle Branched-Chain Amino Acid Metabolism in Nile Tilapia (Oreochromis niloticus) During Starvation
The branched-chain amino acids (BCAAs) play a key role in the energy metabolism of the muscle tissue and the Krüppel-like factor 15 (KLF15) as a transcription factor, which is a key regulator of BCAA metabolism in the skeletal muscle. This study assessed the effect of starvation for 0, 3, 7, and 15...
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Frontiers Media S.A.
2020-08-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fgene.2020.00852/full |
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| author | Honghui Li Honghui Li Xiaoling An Lingsheng Bao Yulong Li Yaxiong Pan Jinggang He Li Liu Xin Zhu Jianshe Zhang Jia Cheng Wuying Chu |
| author_facet | Honghui Li Honghui Li Xiaoling An Lingsheng Bao Yulong Li Yaxiong Pan Jinggang He Li Liu Xin Zhu Jianshe Zhang Jia Cheng Wuying Chu |
| author_sort | Honghui Li |
| collection | DOAJ |
| description | The branched-chain amino acids (BCAAs) play a key role in the energy metabolism of the muscle tissue and the Krüppel-like factor 15 (KLF15) as a transcription factor, which is a key regulator of BCAA metabolism in the skeletal muscle. This study assessed the effect of starvation for 0, 3, 7, and 15 days on BCAA metabolism in the skeletal muscle of Nile tilapia. The results showed that the expression of KLF15 showed a trend of increasing first and then decreasing during starvation, as well as the expression and activity of branched-chain aminotransferase 2 (BCAT2) and alanine aminotransferase (ALT). On the other hand, the content of BCAA was at first decreased and then upregulated, and it reached the lowest level after starvation for 3 days. In addition, through dual-luciferase reporter assay and injection experiments, it was found that KLF15 is the target gene of miR-125a-3p, which further verified that miR-125a-3p can regulate the BCAA metabolism by targeting KLF15 in the skeletal muscle. Thus, our work investigated the possible mechanisms of BCAA metabolism adapting to nutritional deficiency in the skeletal muscle of Nile tilapia and illustrated the regulation of BCAA metabolism through the miR-125a-3p-KLF15-BCAA pathway in the skeletal muscle. |
| first_indexed | 2024-12-13T03:44:19Z |
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| id | doaj.art-b4121f7e102c4bc49fd6d16a84ab6c5a |
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| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-12-13T03:44:19Z |
| publishDate | 2020-08-01 |
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| series | Frontiers in Genetics |
| spelling | doaj.art-b4121f7e102c4bc49fd6d16a84ab6c5a2022-12-22T00:00:52ZengFrontiers Media S.A.Frontiers in Genetics1664-80212020-08-011110.3389/fgene.2020.00852543068MiR-125a-3p-KLF15-BCAA Regulates the Skeletal Muscle Branched-Chain Amino Acid Metabolism in Nile Tilapia (Oreochromis niloticus) During StarvationHonghui Li0Honghui Li1Xiaoling An2Lingsheng Bao3Yulong Li4Yaxiong Pan5Jinggang He6Li Liu7Xin Zhu8Jianshe Zhang9Jia Cheng10Wuying Chu11Hunan Provincial Key Laboratory of Nutrition and Quality Control of Aquatic Animals, College of Biological and Environmental Engineering, Changsha University, Changsha, ChinaGuangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou, ChinaHunan Provincial Key Laboratory of Nutrition and Quality Control of Aquatic Animals, College of Biological and Environmental Engineering, Changsha University, Changsha, ChinaHunan Provincial Key Laboratory of Nutrition and Quality Control of Aquatic Animals, College of Biological and Environmental Engineering, Changsha University, Changsha, ChinaHunan Provincial Key Laboratory of Nutrition and Quality Control of Aquatic Animals, College of Biological and Environmental Engineering, Changsha University, Changsha, ChinaHunan Provincial Key Laboratory of Nutrition and Quality Control of Aquatic Animals, College of Biological and Environmental Engineering, Changsha University, Changsha, ChinaHunan Provincial Key Laboratory of Nutrition and Quality Control of Aquatic Animals, College of Biological and Environmental Engineering, Changsha University, Changsha, ChinaHunan Fisheries Science Institute, Changsha, ChinaHunan Provincial Key Laboratory of Nutrition and Quality Control of Aquatic Animals, College of Biological and Environmental Engineering, Changsha University, Changsha, ChinaHunan Provincial Key Laboratory of Nutrition and Quality Control of Aquatic Animals, College of Biological and Environmental Engineering, Changsha University, Changsha, ChinaHunan Provincial Key Laboratory of Nutrition and Quality Control of Aquatic Animals, College of Biological and Environmental Engineering, Changsha University, Changsha, ChinaHunan Provincial Key Laboratory of Nutrition and Quality Control of Aquatic Animals, College of Biological and Environmental Engineering, Changsha University, Changsha, ChinaThe branched-chain amino acids (BCAAs) play a key role in the energy metabolism of the muscle tissue and the Krüppel-like factor 15 (KLF15) as a transcription factor, which is a key regulator of BCAA metabolism in the skeletal muscle. This study assessed the effect of starvation for 0, 3, 7, and 15 days on BCAA metabolism in the skeletal muscle of Nile tilapia. The results showed that the expression of KLF15 showed a trend of increasing first and then decreasing during starvation, as well as the expression and activity of branched-chain aminotransferase 2 (BCAT2) and alanine aminotransferase (ALT). On the other hand, the content of BCAA was at first decreased and then upregulated, and it reached the lowest level after starvation for 3 days. In addition, through dual-luciferase reporter assay and injection experiments, it was found that KLF15 is the target gene of miR-125a-3p, which further verified that miR-125a-3p can regulate the BCAA metabolism by targeting KLF15 in the skeletal muscle. Thus, our work investigated the possible mechanisms of BCAA metabolism adapting to nutritional deficiency in the skeletal muscle of Nile tilapia and illustrated the regulation of BCAA metabolism through the miR-125a-3p-KLF15-BCAA pathway in the skeletal muscle.https://www.frontiersin.org/article/10.3389/fgene.2020.00852/fullOreochromis niloticusmiR-125a-3p-KLF15-BCAAskeletal musclebranched-chain amino acid metabolismstarvation |
| spellingShingle | Honghui Li Honghui Li Xiaoling An Lingsheng Bao Yulong Li Yaxiong Pan Jinggang He Li Liu Xin Zhu Jianshe Zhang Jia Cheng Wuying Chu MiR-125a-3p-KLF15-BCAA Regulates the Skeletal Muscle Branched-Chain Amino Acid Metabolism in Nile Tilapia (Oreochromis niloticus) During Starvation Frontiers in Genetics Oreochromis niloticus miR-125a-3p-KLF15-BCAA skeletal muscle branched-chain amino acid metabolism starvation |
| title | MiR-125a-3p-KLF15-BCAA Regulates the Skeletal Muscle Branched-Chain Amino Acid Metabolism in Nile Tilapia (Oreochromis niloticus) During Starvation |
| title_full | MiR-125a-3p-KLF15-BCAA Regulates the Skeletal Muscle Branched-Chain Amino Acid Metabolism in Nile Tilapia (Oreochromis niloticus) During Starvation |
| title_fullStr | MiR-125a-3p-KLF15-BCAA Regulates the Skeletal Muscle Branched-Chain Amino Acid Metabolism in Nile Tilapia (Oreochromis niloticus) During Starvation |
| title_full_unstemmed | MiR-125a-3p-KLF15-BCAA Regulates the Skeletal Muscle Branched-Chain Amino Acid Metabolism in Nile Tilapia (Oreochromis niloticus) During Starvation |
| title_short | MiR-125a-3p-KLF15-BCAA Regulates the Skeletal Muscle Branched-Chain Amino Acid Metabolism in Nile Tilapia (Oreochromis niloticus) During Starvation |
| title_sort | mir 125a 3p klf15 bcaa regulates the skeletal muscle branched chain amino acid metabolism in nile tilapia oreochromis niloticus during starvation |
| topic | Oreochromis niloticus miR-125a-3p-KLF15-BCAA skeletal muscle branched-chain amino acid metabolism starvation |
| url | https://www.frontiersin.org/article/10.3389/fgene.2020.00852/full |
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