Production and Optimization of Conjugated Linoleic and Eicosapentaenoic Acids by Bifidobacterium lactis in Cold-Pressed Soybean Cake
Background and PurposeIn regard to the biosynthesis of conjugated linoleic acid (CLA) and eicosapentaenoic acid (EPA) by some bacteria, the objective of this study was to evaluate the efficiency of solid-state fermentation based on soybean pressed cake (SPC) to produce CLA and EPA by Bifidobacterium...
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| Format: | Article |
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Frontiers Media S.A.
2022-07-01
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| Series: | Frontiers in Nutrition |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2022.916728/full |
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| author | Samin Rafi Azari Mohammad Hojjatoleslamy Zeinab E. Mousavi Zeinab E. Mousavi Hossein Kiani Hossein Kiani Sayed Mohammad Ali Jalali Sayed Mohammad Ali Jalali |
| author_facet | Samin Rafi Azari Mohammad Hojjatoleslamy Zeinab E. Mousavi Zeinab E. Mousavi Hossein Kiani Hossein Kiani Sayed Mohammad Ali Jalali Sayed Mohammad Ali Jalali |
| author_sort | Samin Rafi Azari |
| collection | DOAJ |
| description | Background and PurposeIn regard to the biosynthesis of conjugated linoleic acid (CLA) and eicosapentaenoic acid (EPA) by some bacteria, the objective of this study was to evaluate the efficiency of solid-state fermentation based on soybean pressed cake (SPC) to produce CLA and EPA by Bifidobacterium lactis. The objective of this study was to evaluate the efficiency of solid-state fermentation based on SPC to produce CLA and EPA by B. lactis.MethodsProcess conditions including humidity, inoculation level, and temperature parameters were optimized by adopting the response surface methodology (RSM) method (response surface method) and the design expert software. Accordingly, a homogeneous SPC paste substrate at 60, 70, and 80% humidity was prepared with different inoculation levels at 30, 37, and 44°C to assess the strain behavior. The introduced SPC consisted of 60% humidity, 2% inoculation level at 37°C, and 60% humidity, and 4% inoculation level at 30 and 44°C; it also included 6% inoculation level at 37°C, 70% humidity at 2% inoculation level, at 30 and 44°C, and 4% inoculation level at 37°C. Also, SPC with 80% humidity at 2% and 4% inoculation levels, and at 30 and 44°C was obtained. To confirm the accuracy of the conditions, an experiment was conducted according to the defined requirements.ResultsThe results were compared with the predicted data, which showed a significant difference. Under optimized conditions, with an inoculation level of 4% on the SPC medium with 70% humidity and at 37°C, B. lactis strains could yield 9cis-, 11 trans-linoleic and eicosapentaenoic at 0.18 and 0.39% of the total fatty acids.ConclusionSo, the potential benefits of using SPC as an inexpensive substrate for the commercial production of CLA and EPA should be noted. |
| first_indexed | 2024-12-11T16:36:25Z |
| format | Article |
| id | doaj.art-b412cc22202b4848a84bf52a5a995c3b |
| institution | Directory Open Access Journal |
| issn | 2296-861X |
| language | English |
| last_indexed | 2024-12-11T16:36:25Z |
| publishDate | 2022-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nutrition |
| spelling | doaj.art-b412cc22202b4848a84bf52a5a995c3b2022-12-22T00:58:27ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2022-07-01910.3389/fnut.2022.916728916728Production and Optimization of Conjugated Linoleic and Eicosapentaenoic Acids by Bifidobacterium lactis in Cold-Pressed Soybean CakeSamin Rafi Azari0Mohammad Hojjatoleslamy1Zeinab E. Mousavi2Zeinab E. Mousavi3Hossein Kiani4Hossein Kiani5Sayed Mohammad Ali Jalali6Sayed Mohammad Ali Jalali7Department of Food Science and Technology, Shahrekord Branch, Islamic Azad University, Shahrekord, IranDepartment of Food Science and Technology, Shahrekord Branch, Islamic Azad University, Shahrekord, IranDepartment of Food Science and Technology, Shahrekord Branch, Islamic Azad University, Shahrekord, IranBioprocessing and Biodetection Laboratory, Department of Food Science and Engineering, Campus of Agriculture and Natural Resources, University of Tehran, Karaj, IranDepartment of Food Science and Technology, Shahrekord Branch, Islamic Azad University, Shahrekord, IranBioprocessing and Biodetection Laboratory, Department of Food Science and Engineering, Campus of Agriculture and Natural Resources, University of Tehran, Karaj, IranDepartment of Animal Sciences, Faculty of Agriculture and Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, IranResearch Center of Nutrition and Organic Products, Shahrekord Branch, Islamic Azad University, Shahrekord, IranBackground and PurposeIn regard to the biosynthesis of conjugated linoleic acid (CLA) and eicosapentaenoic acid (EPA) by some bacteria, the objective of this study was to evaluate the efficiency of solid-state fermentation based on soybean pressed cake (SPC) to produce CLA and EPA by Bifidobacterium lactis. The objective of this study was to evaluate the efficiency of solid-state fermentation based on SPC to produce CLA and EPA by B. lactis.MethodsProcess conditions including humidity, inoculation level, and temperature parameters were optimized by adopting the response surface methodology (RSM) method (response surface method) and the design expert software. Accordingly, a homogeneous SPC paste substrate at 60, 70, and 80% humidity was prepared with different inoculation levels at 30, 37, and 44°C to assess the strain behavior. The introduced SPC consisted of 60% humidity, 2% inoculation level at 37°C, and 60% humidity, and 4% inoculation level at 30 and 44°C; it also included 6% inoculation level at 37°C, 70% humidity at 2% inoculation level, at 30 and 44°C, and 4% inoculation level at 37°C. Also, SPC with 80% humidity at 2% and 4% inoculation levels, and at 30 and 44°C was obtained. To confirm the accuracy of the conditions, an experiment was conducted according to the defined requirements.ResultsThe results were compared with the predicted data, which showed a significant difference. Under optimized conditions, with an inoculation level of 4% on the SPC medium with 70% humidity and at 37°C, B. lactis strains could yield 9cis-, 11 trans-linoleic and eicosapentaenoic at 0.18 and 0.39% of the total fatty acids.ConclusionSo, the potential benefits of using SPC as an inexpensive substrate for the commercial production of CLA and EPA should be noted.https://www.frontiersin.org/articles/10.3389/fnut.2022.916728/fullconjugated linoleic acid (CLA)EPABifidobacterium lactissoybean pressed cakeRSM |
| spellingShingle | Samin Rafi Azari Mohammad Hojjatoleslamy Zeinab E. Mousavi Zeinab E. Mousavi Hossein Kiani Hossein Kiani Sayed Mohammad Ali Jalali Sayed Mohammad Ali Jalali Production and Optimization of Conjugated Linoleic and Eicosapentaenoic Acids by Bifidobacterium lactis in Cold-Pressed Soybean Cake Frontiers in Nutrition conjugated linoleic acid (CLA) EPA Bifidobacterium lactis soybean pressed cake RSM |
| title | Production and Optimization of Conjugated Linoleic and Eicosapentaenoic Acids by Bifidobacterium lactis in Cold-Pressed Soybean Cake |
| title_full | Production and Optimization of Conjugated Linoleic and Eicosapentaenoic Acids by Bifidobacterium lactis in Cold-Pressed Soybean Cake |
| title_fullStr | Production and Optimization of Conjugated Linoleic and Eicosapentaenoic Acids by Bifidobacterium lactis in Cold-Pressed Soybean Cake |
| title_full_unstemmed | Production and Optimization of Conjugated Linoleic and Eicosapentaenoic Acids by Bifidobacterium lactis in Cold-Pressed Soybean Cake |
| title_short | Production and Optimization of Conjugated Linoleic and Eicosapentaenoic Acids by Bifidobacterium lactis in Cold-Pressed Soybean Cake |
| title_sort | production and optimization of conjugated linoleic and eicosapentaenoic acids by bifidobacterium lactis in cold pressed soybean cake |
| topic | conjugated linoleic acid (CLA) EPA Bifidobacterium lactis soybean pressed cake RSM |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2022.916728/full |
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