Investigation and detection of cyanobacterial Cr-phycoerythrin by laser based techniques
The application of a high-sensitivity method of time resolved laser induced fluorescence (TR-LIF) and Flow-injection system by thermal lens spectrometry (FIA-TLS) for the analysis of Cr-Phycoerythrin (Cr-PE) isolated from a proprietary cyanobacterium is presented. In the excitation waveleng...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Serbian Chemical Society
2014-01-01
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| Series: | Journal of the Serbian Chemical Society |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0352-5139/2014/0352-51391300088M.pdf |
| Summary: | The application of a high-sensitivity method of time resolved laser induced
fluorescence (TR-LIF) and Flow-injection system by thermal lens spectrometry
(FIA-TLS) for the analysis of Cr-Phycoerythrin (Cr-PE) isolated from a
proprietary cyanobacterium is presented. In the excitation wavelength range
(340-470 nm) fluorescent spectra exhibit a pronounced maximum at 575 nm.
Another maximum, at about 600 nm can be also observed. The obtained results
are used to verify the technical parameters of the used thermal lens
technique, which is complementary to spectrofluorimetry and subject to lower
sensitivity in case of high fluorescence quantum yields and photolability of
measured compounds. [Projects MES RS OI 171020 and COST Action FA0906] |
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| ISSN: | 0352-5139 1820-7421 |