Investigation and detection of cyanobacterial Cr-phycoerythrin by laser based techniques
The application of a high-sensitivity method of time resolved laser induced fluorescence (TR-LIF) and Flow-injection system by thermal lens spectrometry (FIA-TLS) for the analysis of Cr-Phycoerythrin (Cr-PE) isolated from a proprietary cyanobacterium is presented. In the excitation waveleng...
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Format: | Article |
Language: | English |
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Serbian Chemical Society
2014-01-01
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Series: | Journal of the Serbian Chemical Society |
Subjects: | |
Online Access: | http://www.doiserbia.nb.rs/img/doi/0352-5139/2014/0352-51391300088M.pdf |
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author | Marinković Bratislav P. Delneri Ambra Rabasović Maja S. Terzić Mira Franko Mladen Šević Dragutin |
author_facet | Marinković Bratislav P. Delneri Ambra Rabasović Maja S. Terzić Mira Franko Mladen Šević Dragutin |
author_sort | Marinković Bratislav P. |
collection | DOAJ |
description | The application of a high-sensitivity method of time resolved laser induced
fluorescence (TR-LIF) and Flow-injection system by thermal lens spectrometry
(FIA-TLS) for the analysis of Cr-Phycoerythrin (Cr-PE) isolated from a
proprietary cyanobacterium is presented. In the excitation wavelength range
(340-470 nm) fluorescent spectra exhibit a pronounced maximum at 575 nm.
Another maximum, at about 600 nm can be also observed. The obtained results
are used to verify the technical parameters of the used thermal lens
technique, which is complementary to spectrofluorimetry and subject to lower
sensitivity in case of high fluorescence quantum yields and photolability of
measured compounds. [Projects MES RS OI 171020 and COST Action FA0906] |
first_indexed | 2024-12-11T04:23:25Z |
format | Article |
id | doaj.art-b417b464033342f59b9e44b9388b7120 |
institution | Directory Open Access Journal |
issn | 0352-5139 1820-7421 |
language | English |
last_indexed | 2024-12-11T04:23:25Z |
publishDate | 2014-01-01 |
publisher | Serbian Chemical Society |
record_format | Article |
series | Journal of the Serbian Chemical Society |
spelling | doaj.art-b417b464033342f59b9e44b9388b71202022-12-22T01:21:03ZengSerbian Chemical SocietyJournal of the Serbian Chemical Society0352-51391820-74212014-01-0179218519810.2298/JSC130417088M0352-51391300088MInvestigation and detection of cyanobacterial Cr-phycoerythrin by laser based techniquesMarinković Bratislav P.0Delneri Ambra1Rabasović Maja S.2Terzić Mira3Franko Mladen4Šević Dragutin5University of Belgrade, Institute of Physics, Belgrade, SerbiaUniversity of Nova Gorica, Laboratory for Environmental Research, Nova Gorica, SloveniaUniversity of Belgrade, Institute of Physics, Belgrade, SerbiaUniversity of Novi Sad, Faculty of Science, Novi Sad, SerbiaUniversity of Nova Gorica, Laboratory for Environmental Research, Nova Gorica, SloveniaUniversity of Belgrade, Institute of Physics, Belgrade, SerbiaThe application of a high-sensitivity method of time resolved laser induced fluorescence (TR-LIF) and Flow-injection system by thermal lens spectrometry (FIA-TLS) for the analysis of Cr-Phycoerythrin (Cr-PE) isolated from a proprietary cyanobacterium is presented. In the excitation wavelength range (340-470 nm) fluorescent spectra exhibit a pronounced maximum at 575 nm. Another maximum, at about 600 nm can be also observed. The obtained results are used to verify the technical parameters of the used thermal lens technique, which is complementary to spectrofluorimetry and subject to lower sensitivity in case of high fluorescence quantum yields and photolability of measured compounds. [Projects MES RS OI 171020 and COST Action FA0906]http://www.doiserbia.nb.rs/img/doi/0352-5139/2014/0352-51391300088M.pdftime resolved laser induced fluorescence spectroscopythermal lens spectroscopyphycoerythrincyanobacteria |
spellingShingle | Marinković Bratislav P. Delneri Ambra Rabasović Maja S. Terzić Mira Franko Mladen Šević Dragutin Investigation and detection of cyanobacterial Cr-phycoerythrin by laser based techniques Journal of the Serbian Chemical Society time resolved laser induced fluorescence spectroscopy thermal lens spectroscopy phycoerythrin cyanobacteria |
title | Investigation and detection of cyanobacterial Cr-phycoerythrin by laser based techniques |
title_full | Investigation and detection of cyanobacterial Cr-phycoerythrin by laser based techniques |
title_fullStr | Investigation and detection of cyanobacterial Cr-phycoerythrin by laser based techniques |
title_full_unstemmed | Investigation and detection of cyanobacterial Cr-phycoerythrin by laser based techniques |
title_short | Investigation and detection of cyanobacterial Cr-phycoerythrin by laser based techniques |
title_sort | investigation and detection of cyanobacterial cr phycoerythrin by laser based techniques |
topic | time resolved laser induced fluorescence spectroscopy thermal lens spectroscopy phycoerythrin cyanobacteria |
url | http://www.doiserbia.nb.rs/img/doi/0352-5139/2014/0352-51391300088M.pdf |
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