Investigation and detection of cyanobacterial Cr-phycoerythrin by laser based techniques

The application of a high-sensitivity method of time resolved laser induced fluorescence (TR-LIF) and Flow-injection system by thermal lens spectrometry (FIA-TLS) for the analysis of Cr-Phycoerythrin (Cr-PE) isolated from a proprietary cyanobacterium is presented. In the excitation waveleng...

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Main Authors: Marinković Bratislav P., Delneri Ambra, Rabasović Maja S., Terzić Mira, Franko Mladen, Šević Dragutin
Format: Article
Language:English
Published: Serbian Chemical Society 2014-01-01
Series:Journal of the Serbian Chemical Society
Subjects:
Online Access:http://www.doiserbia.nb.rs/img/doi/0352-5139/2014/0352-51391300088M.pdf
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author Marinković Bratislav P.
Delneri Ambra
Rabasović Maja S.
Terzić Mira
Franko Mladen
Šević Dragutin
author_facet Marinković Bratislav P.
Delneri Ambra
Rabasović Maja S.
Terzić Mira
Franko Mladen
Šević Dragutin
author_sort Marinković Bratislav P.
collection DOAJ
description The application of a high-sensitivity method of time resolved laser induced fluorescence (TR-LIF) and Flow-injection system by thermal lens spectrometry (FIA-TLS) for the analysis of Cr-Phycoerythrin (Cr-PE) isolated from a proprietary cyanobacterium is presented. In the excitation wavelength range (340-470 nm) fluorescent spectra exhibit a pronounced maximum at 575 nm. Another maximum, at about 600 nm can be also observed. The obtained results are used to verify the technical parameters of the used thermal lens technique, which is complementary to spectrofluorimetry and subject to lower sensitivity in case of high fluorescence quantum yields and photolability of measured compounds. [Projects MES RS OI 171020 and COST Action FA0906]
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spelling doaj.art-b417b464033342f59b9e44b9388b71202022-12-22T01:21:03ZengSerbian Chemical SocietyJournal of the Serbian Chemical Society0352-51391820-74212014-01-0179218519810.2298/JSC130417088M0352-51391300088MInvestigation and detection of cyanobacterial Cr-phycoerythrin by laser based techniquesMarinković Bratislav P.0Delneri Ambra1Rabasović Maja S.2Terzić Mira3Franko Mladen4Šević Dragutin5University of Belgrade, Institute of Physics, Belgrade, SerbiaUniversity of Nova Gorica, Laboratory for Environmental Research, Nova Gorica, SloveniaUniversity of Belgrade, Institute of Physics, Belgrade, SerbiaUniversity of Novi Sad, Faculty of Science, Novi Sad, SerbiaUniversity of Nova Gorica, Laboratory for Environmental Research, Nova Gorica, SloveniaUniversity of Belgrade, Institute of Physics, Belgrade, SerbiaThe application of a high-sensitivity method of time resolved laser induced fluorescence (TR-LIF) and Flow-injection system by thermal lens spectrometry (FIA-TLS) for the analysis of Cr-Phycoerythrin (Cr-PE) isolated from a proprietary cyanobacterium is presented. In the excitation wavelength range (340-470 nm) fluorescent spectra exhibit a pronounced maximum at 575 nm. Another maximum, at about 600 nm can be also observed. The obtained results are used to verify the technical parameters of the used thermal lens technique, which is complementary to spectrofluorimetry and subject to lower sensitivity in case of high fluorescence quantum yields and photolability of measured compounds. [Projects MES RS OI 171020 and COST Action FA0906]http://www.doiserbia.nb.rs/img/doi/0352-5139/2014/0352-51391300088M.pdftime resolved laser induced fluorescence spectroscopythermal lens spectroscopyphycoerythrincyanobacteria
spellingShingle Marinković Bratislav P.
Delneri Ambra
Rabasović Maja S.
Terzić Mira
Franko Mladen
Šević Dragutin
Investigation and detection of cyanobacterial Cr-phycoerythrin by laser based techniques
Journal of the Serbian Chemical Society
time resolved laser induced fluorescence spectroscopy
thermal lens spectroscopy
phycoerythrin
cyanobacteria
title Investigation and detection of cyanobacterial Cr-phycoerythrin by laser based techniques
title_full Investigation and detection of cyanobacterial Cr-phycoerythrin by laser based techniques
title_fullStr Investigation and detection of cyanobacterial Cr-phycoerythrin by laser based techniques
title_full_unstemmed Investigation and detection of cyanobacterial Cr-phycoerythrin by laser based techniques
title_short Investigation and detection of cyanobacterial Cr-phycoerythrin by laser based techniques
title_sort investigation and detection of cyanobacterial cr phycoerythrin by laser based techniques
topic time resolved laser induced fluorescence spectroscopy
thermal lens spectroscopy
phycoerythrin
cyanobacteria
url http://www.doiserbia.nb.rs/img/doi/0352-5139/2014/0352-51391300088M.pdf
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AT rabasovicmajas investigationanddetectionofcyanobacterialcrphycoerythrinbylaserbasedtechniques
AT terzicmira investigationanddetectionofcyanobacterialcrphycoerythrinbylaserbasedtechniques
AT frankomladen investigationanddetectionofcyanobacterialcrphycoerythrinbylaserbasedtechniques
AT sevicdragutin investigationanddetectionofcyanobacterialcrphycoerythrinbylaserbasedtechniques