Comparative transcriptomic profiling of High- and Low- grain Zinc and Iron containing Indian wheat genotypes
Development of minerals rich grains with stress tolerance response (STR) wheat cultivars will help to eradicate malnutrition problem globally. The current study deals with the comparative transcriptome analysis of 4 High- and 3 Low- grain Zinc (Zn) & Iron (Fe) containing wheat genotypes (HZFWGs,...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2019-05-01
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| Series: | Current Plant Biology |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214662819300490 |
| _version_ | 1831760766929207296 |
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| author | Vinod Kumar Mishra Saurabh Gupta Ramesh Chand Punam Singh Yadav Satish Kumar Singh Arun Kumar Joshi Pritish Kumar Varadwaj |
| author_facet | Vinod Kumar Mishra Saurabh Gupta Ramesh Chand Punam Singh Yadav Satish Kumar Singh Arun Kumar Joshi Pritish Kumar Varadwaj |
| author_sort | Vinod Kumar Mishra |
| collection | DOAJ |
| description | Development of minerals rich grains with stress tolerance response (STR) wheat cultivars will help to eradicate malnutrition problem globally. The current study deals with the comparative transcriptome analysis of 4 High- and 3 Low- grain Zinc (Zn) & Iron (Fe) containing wheat genotypes (HZFWGs, LZFWGs) to elucidate the genes responsible for mineral transportation mechanism and stress tolerance response(STR). Differentially expressed genes (DEGs) and their functional role in metabolic pathways of mineral transportation, as well as other important pathways, have been explored. Gene ontology (GO) and pathways analysis revealed that phenylpropanoid biosynthesis and other associated pathways are mainly involved in plant stress defense mechanism in both genotypes. Chlorophyll synthesis, purine metabolism, Zn & Fe binding, metal ion transport, and ATP-Synthase coupled transport mechanism are highly active in HZFWGs while ribosomal formation, biomolecules binding activities and secondary metabolite biosynthesis in LZFWGs. It was also found that genes accountable for minerals uptake and purine metabolism in HZFWGs are highly enriched. The genes identified in this study may be used for marker-assisted selection and breeding to develop minerals rich crops. Keywords: Malnutrition, Minerals uptake, Wheat, Metabolic pathways, Phenylpropanoid biosynthesis |
| first_indexed | 2024-12-22T04:37:14Z |
| format | Article |
| id | doaj.art-b417cceab12a424aacd8c2bfcb114f0e |
| institution | Directory Open Access Journal |
| issn | 2214-6628 |
| language | English |
| last_indexed | 2024-12-22T04:37:14Z |
| publishDate | 2019-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Current Plant Biology |
| spelling | doaj.art-b417cceab12a424aacd8c2bfcb114f0e2022-12-21T18:38:52ZengElsevierCurrent Plant Biology2214-66282019-05-0118Comparative transcriptomic profiling of High- and Low- grain Zinc and Iron containing Indian wheat genotypesVinod Kumar Mishra0Saurabh Gupta1Ramesh Chand2Punam Singh Yadav3Satish Kumar Singh4Arun Kumar Joshi5Pritish Kumar Varadwaj6Institute of Agricultural Sciences, Banaras Hindu University, Varanasi-221005, IndiaDepartment of Applied Sciences, Indian Institute of Information Technology, Allahabad-211015, India; AgriGenome Labs Pvt. Ltd., Genome Valley, Hyderabad-500078, India; Corresponding author at: Scientist, AgriGenome Labs Pvt. Ltd., G5, BTIC, MN iHub, Genome Valley, Shamirpet, Hyderabad-500078, Telangana State, India.Institute of Agricultural Sciences, Banaras Hindu University, Varanasi-221005, IndiaInstitute of Agricultural Sciences, Banaras Hindu University, Varanasi-221005, IndiaInstitute of Agricultural Sciences, Banaras Hindu University, Varanasi-221005, IndiaInternational Maize and Wheat Improvement Center (CIMMYT), NASC complex, DPS Marg, New Delhi-110012, India; Borlaug Institute for South Asia (BISA), NASC Complex, DPS Marg, New Delhi-110012, IndiaDepartment of Applied Sciences, Indian Institute of Information Technology, Allahabad-211015, India; Corresponding author at: Associate Professor, Department of Applied Sciences, Indian Institute of Information Technology, Allahabad, Devghat, Jhalwa, Allahabad-211015, U. P., India.Development of minerals rich grains with stress tolerance response (STR) wheat cultivars will help to eradicate malnutrition problem globally. The current study deals with the comparative transcriptome analysis of 4 High- and 3 Low- grain Zinc (Zn) & Iron (Fe) containing wheat genotypes (HZFWGs, LZFWGs) to elucidate the genes responsible for mineral transportation mechanism and stress tolerance response(STR). Differentially expressed genes (DEGs) and their functional role in metabolic pathways of mineral transportation, as well as other important pathways, have been explored. Gene ontology (GO) and pathways analysis revealed that phenylpropanoid biosynthesis and other associated pathways are mainly involved in plant stress defense mechanism in both genotypes. Chlorophyll synthesis, purine metabolism, Zn & Fe binding, metal ion transport, and ATP-Synthase coupled transport mechanism are highly active in HZFWGs while ribosomal formation, biomolecules binding activities and secondary metabolite biosynthesis in LZFWGs. It was also found that genes accountable for minerals uptake and purine metabolism in HZFWGs are highly enriched. The genes identified in this study may be used for marker-assisted selection and breeding to develop minerals rich crops. Keywords: Malnutrition, Minerals uptake, Wheat, Metabolic pathways, Phenylpropanoid biosynthesishttp://www.sciencedirect.com/science/article/pii/S2214662819300490 |
| spellingShingle | Vinod Kumar Mishra Saurabh Gupta Ramesh Chand Punam Singh Yadav Satish Kumar Singh Arun Kumar Joshi Pritish Kumar Varadwaj Comparative transcriptomic profiling of High- and Low- grain Zinc and Iron containing Indian wheat genotypes Current Plant Biology |
| title | Comparative transcriptomic profiling of High- and Low- grain Zinc and Iron containing Indian wheat genotypes |
| title_full | Comparative transcriptomic profiling of High- and Low- grain Zinc and Iron containing Indian wheat genotypes |
| title_fullStr | Comparative transcriptomic profiling of High- and Low- grain Zinc and Iron containing Indian wheat genotypes |
| title_full_unstemmed | Comparative transcriptomic profiling of High- and Low- grain Zinc and Iron containing Indian wheat genotypes |
| title_short | Comparative transcriptomic profiling of High- and Low- grain Zinc and Iron containing Indian wheat genotypes |
| title_sort | comparative transcriptomic profiling of high and low grain zinc and iron containing indian wheat genotypes |
| url | http://www.sciencedirect.com/science/article/pii/S2214662819300490 |
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