Recent Advances in Cell Adhesive Force Microscopy
Cell adhesive force, exerting on the local matrix or neighboring cells, plays a critical role in regulating many cell functions and physiological processes. In the past four decades, significant efforts have been dedicated to cell adhesive force detection, visualization and quantification. A recent...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-12-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/20/24/7128 |
| _version_ | 1827700206613823488 |
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| author | Ying Tu Xuefeng Wang |
| author_facet | Ying Tu Xuefeng Wang |
| author_sort | Ying Tu |
| collection | DOAJ |
| description | Cell adhesive force, exerting on the local matrix or neighboring cells, plays a critical role in regulating many cell functions and physiological processes. In the past four decades, significant efforts have been dedicated to cell adhesive force detection, visualization and quantification. A recent important methodological advancement in cell adhesive force visualization is to adopt force-to-fluorescence conversion instead of force-to-substrate strain conversion, thus greatly improving the sensitivity and resolution of force imaging. This review summarizes the recent development of force imaging techniques (collectively termed as cell adhesive force microscopy or CAFM here), with a particular focus on the improvement of CAFM’s spatial resolution and the biomaterial choices for constructing the tension sensors used in force visualization. This review also highlights the importance of DNA-based tension sensors in cell adhesive force imaging and the recent breakthrough in the development of super-resolution CAFM. |
| first_indexed | 2024-03-10T14:08:03Z |
| format | Article |
| id | doaj.art-b419a0b074cd418c8e90312b6990faa4 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-03-10T14:08:03Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-b419a0b074cd418c8e90312b6990faa42023-11-21T00:30:58ZengMDPI AGSensors1424-82202020-12-012024712810.3390/s20247128Recent Advances in Cell Adhesive Force MicroscopyYing Tu0Xuefeng Wang1Department of Physics and Astronomy, Iowa State University, Ames, IA 50011, USADepartment of Physics and Astronomy, Iowa State University, Ames, IA 50011, USACell adhesive force, exerting on the local matrix or neighboring cells, plays a critical role in regulating many cell functions and physiological processes. In the past four decades, significant efforts have been dedicated to cell adhesive force detection, visualization and quantification. A recent important methodological advancement in cell adhesive force visualization is to adopt force-to-fluorescence conversion instead of force-to-substrate strain conversion, thus greatly improving the sensitivity and resolution of force imaging. This review summarizes the recent development of force imaging techniques (collectively termed as cell adhesive force microscopy or CAFM here), with a particular focus on the improvement of CAFM’s spatial resolution and the biomaterial choices for constructing the tension sensors used in force visualization. This review also highlights the importance of DNA-based tension sensors in cell adhesive force imaging and the recent breakthrough in the development of super-resolution CAFM.https://www.mdpi.com/1424-8220/20/24/7128integrincell adhesioncell adhesive forcecell traction forceintegrin tensiontension sensor |
| spellingShingle | Ying Tu Xuefeng Wang Recent Advances in Cell Adhesive Force Microscopy Sensors integrin cell adhesion cell adhesive force cell traction force integrin tension tension sensor |
| title | Recent Advances in Cell Adhesive Force Microscopy |
| title_full | Recent Advances in Cell Adhesive Force Microscopy |
| title_fullStr | Recent Advances in Cell Adhesive Force Microscopy |
| title_full_unstemmed | Recent Advances in Cell Adhesive Force Microscopy |
| title_short | Recent Advances in Cell Adhesive Force Microscopy |
| title_sort | recent advances in cell adhesive force microscopy |
| topic | integrin cell adhesion cell adhesive force cell traction force integrin tension tension sensor |
| url | https://www.mdpi.com/1424-8220/20/24/7128 |
| work_keys_str_mv | AT yingtu recentadvancesincelladhesiveforcemicroscopy AT xuefengwang recentadvancesincelladhesiveforcemicroscopy |