Tape-disc-loop-mediated isothermal amplification (TD-LAMP) method as noninvasive approach for diagnosis of cutaneous leishmaniasis caused by L. tropica
Cutaneous leishmaniasis (CL) is a parasitic disease caused by the bite of infectious female sand flies with high socioeconomic burdens. There is currently no non-invasive, point-of-care, diagnostic method with high sensitivity and specificity available for CL. We herein report the development of a n...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-11-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S240584402308605X |
| _version_ | 1797429930700570624 |
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| author | Yasaman Taslimi Sima Habibzadeh Vahid Mashayekhi Goyonlo Amin Akbarzadeh Zahra Azarpour Safoora Gharibzadeh Mehrdad Shokouhy Josefine Persson Ali M. Harandi Amir Mizbani Sima Rafati |
| author_facet | Yasaman Taslimi Sima Habibzadeh Vahid Mashayekhi Goyonlo Amin Akbarzadeh Zahra Azarpour Safoora Gharibzadeh Mehrdad Shokouhy Josefine Persson Ali M. Harandi Amir Mizbani Sima Rafati |
| author_sort | Yasaman Taslimi |
| collection | DOAJ |
| description | Cutaneous leishmaniasis (CL) is a parasitic disease caused by the bite of infectious female sand flies with high socioeconomic burdens. There is currently no non-invasive, point-of-care, diagnostic method with high sensitivity and specificity available for CL. We herein report the development of a non-invasive tape disc (TD) sampling method combined with a loop-mediated isothermal amplification (LAMP) assay using primer sets targeting kinetoplast DNA (kDNA) of Leishmania tropica (L. tropica) with a colorimetric readout for species-specific diagnosis of CL. We tested our Tape-Disc (TD)-LAMP method on a panel of skin samples collected by TD from 35 confirmed L. tropica patients, 35 healthy individuals and 35 patients with non-L. tropica infections. The detection limit of the TD-LAMP assay was determined as 1 fg (fg), and the assay sensitivity and specificity of 97 % and 100 % for L. tropica infection, respectively. This non-invasive, sensitive and rapid diagnostic method warrants further exploration of its use for differential diagnosis of CL in disease endemic settings. |
| first_indexed | 2024-03-09T09:20:01Z |
| format | Article |
| id | doaj.art-b41b90c1106d47039f76beeeb07d9374 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-09T09:20:01Z |
| publishDate | 2023-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-b41b90c1106d47039f76beeeb07d93742023-12-02T07:02:07ZengElsevierHeliyon2405-84402023-11-01911e21397Tape-disc-loop-mediated isothermal amplification (TD-LAMP) method as noninvasive approach for diagnosis of cutaneous leishmaniasis caused by L. tropicaYasaman Taslimi0Sima Habibzadeh1Vahid Mashayekhi Goyonlo2Amin Akbarzadeh3Zahra Azarpour4Safoora Gharibzadeh5Mehrdad Shokouhy6Josefine Persson7Ali M. Harandi8Amir Mizbani9Sima Rafati10Department of Immunotherapy and Leishmania Vaccine Research, Pasteur Institute of Iran, Tehran, IranDepartment of Immunotherapy and Leishmania Vaccine Research, Pasteur Institute of Iran, Tehran, IranCutaneous Leishmaniasis Research Center, Mashhad University of Medical Sciences, Mashhad, IranCutaneous Leishmaniasis Research Center, Mashhad University of Medical Sciences, Mashhad, IranCutaneous Leishmaniasis Research Center, Mashhad University of Medical Sciences, Mashhad, IranDepartment of Epidemiology and Biostatistics, Pasteur Institute of Iran, Tehran, IranDepartment of Immunotherapy and Leishmania Vaccine Research, Pasteur Institute of Iran, Tehran, IranDepartment of Microbiology and Immunology, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, SwedenDepartment of Microbiology and Immunology, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, Sweden; Vaccine Evaluation Center, BC Children's Hospital Research Institute, The University of British Columbia, Vancouver, Canada; Corresponding author. Dr. Ali M. Harandi, Department of Microbiology and Immunology, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, Sweden.ETH Zurich, Zurich, SwitzerlandDepartment of Immunotherapy and Leishmania Vaccine Research, Pasteur Institute of Iran, Tehran, Iran; Corresponding author.Cutaneous leishmaniasis (CL) is a parasitic disease caused by the bite of infectious female sand flies with high socioeconomic burdens. There is currently no non-invasive, point-of-care, diagnostic method with high sensitivity and specificity available for CL. We herein report the development of a non-invasive tape disc (TD) sampling method combined with a loop-mediated isothermal amplification (LAMP) assay using primer sets targeting kinetoplast DNA (kDNA) of Leishmania tropica (L. tropica) with a colorimetric readout for species-specific diagnosis of CL. We tested our Tape-Disc (TD)-LAMP method on a panel of skin samples collected by TD from 35 confirmed L. tropica patients, 35 healthy individuals and 35 patients with non-L. tropica infections. The detection limit of the TD-LAMP assay was determined as 1 fg (fg), and the assay sensitivity and specificity of 97 % and 100 % for L. tropica infection, respectively. This non-invasive, sensitive and rapid diagnostic method warrants further exploration of its use for differential diagnosis of CL in disease endemic settings.http://www.sciencedirect.com/science/article/pii/S240584402308605XCutaneous leishmaniasisNon-invasive skin samplingDiagnosisskin tape-discLAMPPoint of care |
| spellingShingle | Yasaman Taslimi Sima Habibzadeh Vahid Mashayekhi Goyonlo Amin Akbarzadeh Zahra Azarpour Safoora Gharibzadeh Mehrdad Shokouhy Josefine Persson Ali M. Harandi Amir Mizbani Sima Rafati Tape-disc-loop-mediated isothermal amplification (TD-LAMP) method as noninvasive approach for diagnosis of cutaneous leishmaniasis caused by L. tropica Heliyon Cutaneous leishmaniasis Non-invasive skin sampling Diagnosis skin tape-disc LAMP Point of care |
| title | Tape-disc-loop-mediated isothermal amplification (TD-LAMP) method as noninvasive approach for diagnosis of cutaneous leishmaniasis caused by L. tropica |
| title_full | Tape-disc-loop-mediated isothermal amplification (TD-LAMP) method as noninvasive approach for diagnosis of cutaneous leishmaniasis caused by L. tropica |
| title_fullStr | Tape-disc-loop-mediated isothermal amplification (TD-LAMP) method as noninvasive approach for diagnosis of cutaneous leishmaniasis caused by L. tropica |
| title_full_unstemmed | Tape-disc-loop-mediated isothermal amplification (TD-LAMP) method as noninvasive approach for diagnosis of cutaneous leishmaniasis caused by L. tropica |
| title_short | Tape-disc-loop-mediated isothermal amplification (TD-LAMP) method as noninvasive approach for diagnosis of cutaneous leishmaniasis caused by L. tropica |
| title_sort | tape disc loop mediated isothermal amplification td lamp method as noninvasive approach for diagnosis of cutaneous leishmaniasis caused by l tropica |
| topic | Cutaneous leishmaniasis Non-invasive skin sampling Diagnosis skin tape-disc LAMP Point of care |
| url | http://www.sciencedirect.com/science/article/pii/S240584402308605X |
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