Optogenetic modulation and multi-electrode analysis of cerebellar networks in vivo.
The firing patterns of cerebellar Purkinje cells (PCs), as the sole output of the cerebellar cortex, determine and tune motor behavior. PC firing is modulated by various inputs from different brain regions and by cell-types including granule cells (GCs), climbing fibers and inhibitory interneurons....
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4140813?pdf=render |
| _version_ | 1818921721321750528 |
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| author | Wolfgang Kruse Martin Krause Janna Aarse Melanie D Mark Denise Manahan-Vaughan Stefan Herlitze |
| author_facet | Wolfgang Kruse Martin Krause Janna Aarse Melanie D Mark Denise Manahan-Vaughan Stefan Herlitze |
| author_sort | Wolfgang Kruse |
| collection | DOAJ |
| description | The firing patterns of cerebellar Purkinje cells (PCs), as the sole output of the cerebellar cortex, determine and tune motor behavior. PC firing is modulated by various inputs from different brain regions and by cell-types including granule cells (GCs), climbing fibers and inhibitory interneurons. To understand how signal integration in PCs occurs and how subtle changes in the modulation of PC firing lead to adjustment of motor behaviors, it is important to precisely record PC firing in vivo and to control modulatory pathways in a spatio-temporal manner. Combining optogenetic and multi-electrode approaches, we established a new method to integrate light-guides into a multi-electrode system. With this method we are able to variably position the light-guide in defined regions relative to the recording electrode with micrometer precision. We show that PC firing can be precisely monitored and modulated by light-activation of channelrhodopsin-2 (ChR2) expressed in PCs, GCs and interneurons. Thus, this method is ideally suited to investigate the spatio/temporal modulation of PCs in anesthetized and in behaving mice. |
| first_indexed | 2024-12-20T01:42:09Z |
| format | Article |
| id | doaj.art-b41ba23f36714ede80d9ba5ecbe8e962 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T01:42:09Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-b41ba23f36714ede80d9ba5ecbe8e9622022-12-21T19:57:53ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0198e10558910.1371/journal.pone.0105589Optogenetic modulation and multi-electrode analysis of cerebellar networks in vivo.Wolfgang KruseMartin KrauseJanna AarseMelanie D MarkDenise Manahan-VaughanStefan HerlitzeThe firing patterns of cerebellar Purkinje cells (PCs), as the sole output of the cerebellar cortex, determine and tune motor behavior. PC firing is modulated by various inputs from different brain regions and by cell-types including granule cells (GCs), climbing fibers and inhibitory interneurons. To understand how signal integration in PCs occurs and how subtle changes in the modulation of PC firing lead to adjustment of motor behaviors, it is important to precisely record PC firing in vivo and to control modulatory pathways in a spatio-temporal manner. Combining optogenetic and multi-electrode approaches, we established a new method to integrate light-guides into a multi-electrode system. With this method we are able to variably position the light-guide in defined regions relative to the recording electrode with micrometer precision. We show that PC firing can be precisely monitored and modulated by light-activation of channelrhodopsin-2 (ChR2) expressed in PCs, GCs and interneurons. Thus, this method is ideally suited to investigate the spatio/temporal modulation of PCs in anesthetized and in behaving mice.http://europepmc.org/articles/PMC4140813?pdf=render |
| spellingShingle | Wolfgang Kruse Martin Krause Janna Aarse Melanie D Mark Denise Manahan-Vaughan Stefan Herlitze Optogenetic modulation and multi-electrode analysis of cerebellar networks in vivo. PLoS ONE |
| title | Optogenetic modulation and multi-electrode analysis of cerebellar networks in vivo. |
| title_full | Optogenetic modulation and multi-electrode analysis of cerebellar networks in vivo. |
| title_fullStr | Optogenetic modulation and multi-electrode analysis of cerebellar networks in vivo. |
| title_full_unstemmed | Optogenetic modulation and multi-electrode analysis of cerebellar networks in vivo. |
| title_short | Optogenetic modulation and multi-electrode analysis of cerebellar networks in vivo. |
| title_sort | optogenetic modulation and multi electrode analysis of cerebellar networks in vivo |
| url | http://europepmc.org/articles/PMC4140813?pdf=render |
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