The use of autologous skeletal muscle progenitor cells for adjunctive treatment of presumptive urethral sphincter mechanism incompetence in female dogs
Abstract Background Urethral sphincter mechanism incompetence (USMI) is a common problem in female dogs, but some dogs fail to achieve continence with standard treatment. Urethral submucosal injection of autologous skeletal muscle progenitor cells (skMPCs) previously has been shown to restore urethr...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-09-01
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| Series: | Journal of Veterinary Internal Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1111/jvim.16505 |
| _version_ | 1811258673480073216 |
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| author | Shelly L. Vaden Kyle G. Mathews James Yoo James Koudy Williams Tonya Harris Patty Secoura James Robertson Katherine L. Gleason Hannah Reynolds Jorge Piedrahita |
| author_facet | Shelly L. Vaden Kyle G. Mathews James Yoo James Koudy Williams Tonya Harris Patty Secoura James Robertson Katherine L. Gleason Hannah Reynolds Jorge Piedrahita |
| author_sort | Shelly L. Vaden |
| collection | DOAJ |
| description | Abstract Background Urethral sphincter mechanism incompetence (USMI) is a common problem in female dogs, but some dogs fail to achieve continence with standard treatment. Urethral submucosal injection of autologous skeletal muscle progenitor cells (skMPCs) previously has been shown to restore urethral function in a canine model of USMI. Hypothesis/Objective To determine if urethral submucosal injection of skMPC alters continence in dogs with USMI that had previously failed standard medical management. We hypothesized that the injections would lead to improved continence. Animals Fifteen client‐owned dogs with USMI that had failed standard medical management. Methods Dogs were prospectively enrolled into a single‐armed clinical trial. Once enrolled, a triceps muscle of each dog was biopsied; the tissue specimens were digested, cultured, and expanded to 100 million cells before injection into the urethral submucosa using a surgical approach. Continence was assessed at baseline and 3, 6, 12, and 24 months post‐injection using continence scores and urethral pressure profilometry. Results Median continence scores increased significantly from baseline at 3, 6, 12, and 24 months. Increases were seen in 14 of 15 dogs with 7, 6 or 1 dog achieving scores of 5, 4 or 3, respectively. Additional medication was required to achieve continence in all but 2 dogs. Conclusions and Clinical Importance Urethral submucosal injection of skMPC can be used adjunctively to improve continence in dogs with difficult to manage USMI. The procedure is labor intensive but well tolerated; most dogs will require continued medication to remain continent. |
| first_indexed | 2024-04-12T18:18:12Z |
| format | Article |
| id | doaj.art-b420d41b2b8441afa2b25748d7257482 |
| institution | Directory Open Access Journal |
| issn | 0891-6640 1939-1676 |
| language | English |
| last_indexed | 2024-04-12T18:18:12Z |
| publishDate | 2022-09-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Veterinary Internal Medicine |
| spelling | doaj.art-b420d41b2b8441afa2b25748d72574822022-12-22T03:21:32ZengWileyJournal of Veterinary Internal Medicine0891-66401939-16762022-09-013651686169210.1111/jvim.16505The use of autologous skeletal muscle progenitor cells for adjunctive treatment of presumptive urethral sphincter mechanism incompetence in female dogsShelly L. Vaden0Kyle G. Mathews1James Yoo2James Koudy Williams3Tonya Harris4Patty Secoura5James Robertson6Katherine L. Gleason7Hannah Reynolds8Jorge Piedrahita9Department of Clinical Sciences North Carolina State University Raleigh North Carolina USADepartment of Clinical Sciences North Carolina State University Raleigh North Carolina USAWake Forest Institute for Regenerative Medicine Winston‐Salem North Carolina USAWake Forest Institute for Regenerative Medicine Winston‐Salem North Carolina USADepartment of Clinical Sciences North Carolina State University Raleigh North Carolina USANC State Veterinary Hospital North Carolina State University Raleigh North Carolina USACollege of Veterinary Medicine North Carolina State University Raleigh North Carolina USADepartment of Molecular Biomedical Sciences North Carolina State University Raleigh North Carolina USADepartment of Molecular Biomedical Sciences North Carolina State University Raleigh North Carolina USADepartment of Molecular Biomedical Sciences North Carolina State University Raleigh North Carolina USAAbstract Background Urethral sphincter mechanism incompetence (USMI) is a common problem in female dogs, but some dogs fail to achieve continence with standard treatment. Urethral submucosal injection of autologous skeletal muscle progenitor cells (skMPCs) previously has been shown to restore urethral function in a canine model of USMI. Hypothesis/Objective To determine if urethral submucosal injection of skMPC alters continence in dogs with USMI that had previously failed standard medical management. We hypothesized that the injections would lead to improved continence. Animals Fifteen client‐owned dogs with USMI that had failed standard medical management. Methods Dogs were prospectively enrolled into a single‐armed clinical trial. Once enrolled, a triceps muscle of each dog was biopsied; the tissue specimens were digested, cultured, and expanded to 100 million cells before injection into the urethral submucosa using a surgical approach. Continence was assessed at baseline and 3, 6, 12, and 24 months post‐injection using continence scores and urethral pressure profilometry. Results Median continence scores increased significantly from baseline at 3, 6, 12, and 24 months. Increases were seen in 14 of 15 dogs with 7, 6 or 1 dog achieving scores of 5, 4 or 3, respectively. Additional medication was required to achieve continence in all but 2 dogs. Conclusions and Clinical Importance Urethral submucosal injection of skMPC can be used adjunctively to improve continence in dogs with difficult to manage USMI. The procedure is labor intensive but well tolerated; most dogs will require continued medication to remain continent.https://doi.org/10.1111/jvim.16505canineregenerative medicinestem cellurinary incontinence |
| spellingShingle | Shelly L. Vaden Kyle G. Mathews James Yoo James Koudy Williams Tonya Harris Patty Secoura James Robertson Katherine L. Gleason Hannah Reynolds Jorge Piedrahita The use of autologous skeletal muscle progenitor cells for adjunctive treatment of presumptive urethral sphincter mechanism incompetence in female dogs Journal of Veterinary Internal Medicine canine regenerative medicine stem cell urinary incontinence |
| title | The use of autologous skeletal muscle progenitor cells for adjunctive treatment of presumptive urethral sphincter mechanism incompetence in female dogs |
| title_full | The use of autologous skeletal muscle progenitor cells for adjunctive treatment of presumptive urethral sphincter mechanism incompetence in female dogs |
| title_fullStr | The use of autologous skeletal muscle progenitor cells for adjunctive treatment of presumptive urethral sphincter mechanism incompetence in female dogs |
| title_full_unstemmed | The use of autologous skeletal muscle progenitor cells for adjunctive treatment of presumptive urethral sphincter mechanism incompetence in female dogs |
| title_short | The use of autologous skeletal muscle progenitor cells for adjunctive treatment of presumptive urethral sphincter mechanism incompetence in female dogs |
| title_sort | use of autologous skeletal muscle progenitor cells for adjunctive treatment of presumptive urethral sphincter mechanism incompetence in female dogs |
| topic | canine regenerative medicine stem cell urinary incontinence |
| url | https://doi.org/10.1111/jvim.16505 |
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