β-Ⅲ-Tubulin:a reliable marker for retinal ganglion cell labeling in experimental models of glaucoma
<b>AIM:</b> To evaluate the reliability of β-III-Tubulin protein as a retinal ganglion cell (RGC) marker in the experimental glaucoma model. <b>METHODS:</b> Glaucoma mouse models were established by injecting polystyrene microbeads into the anterior chamber of C57BL/6J mice,...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Press of International Journal of Ophthalmology (IJO PRESS)
2015-08-01
|
| Series: | International Journal of Ophthalmology |
| Subjects: | |
| Online Access: | http://www.ijo.cn/en_publish/2015/4/20150401.pdf |
| _version_ | 1811246181007753216 |
|---|---|
| author | Shan-Ming Jiang Li-Ping Zeng Ji-Hong Zeng Li Tang Xiao-Ming Chen Xin Wei |
| author_facet | Shan-Ming Jiang Li-Ping Zeng Ji-Hong Zeng Li Tang Xiao-Ming Chen Xin Wei |
| author_sort | Shan-Ming Jiang |
| collection | DOAJ |
| description | <b>AIM:</b> To evaluate the reliability of β-III-Tubulin protein as a retinal ganglion cell (RGC) marker in the experimental glaucoma model.
<b>METHODS:</b> Glaucoma mouse models were established by injecting polystyrene microbeads into the anterior chamber of C57BL/6J mice, then their retinas were obtained 14d and 28d after the intraocular pressure (IOP) was elevated. Retinal flat mounts and sections were double-labeled by fluorogold (FG) and β-III-Tubulin antibody or single-labeled by β-III-Tubulin antibody, then RGCs were counted and compared respectively.
<b>RESULTS:</b> IOP of the injected eyes were elevated significantly and reached the peak at 22.8±0.7 mm Hg by day 14 after injection, then dropped to 11.3±0.7 mm Hg by day 28. RGC numbers counted by FG labeling and β-III-Tubulin antibody labeling were 64 807±4930 and 64614±5054 respectively in the control group, with no significant difference. By day 14, RGCs in the experimental group decreased significantly compared to the control group, but there was no significant difference between the FG labeling counting and the β-III-Tubulin antibody labeling counting either in the experimental group or in the control group. The result was similar by day 28, with further RGC loss.
<b>CONCLUSION:</b> Our result suggested that the β-III-Tubulin protein was not affected by IOP elevation and can be used as a reliable marker for RGC in experimental models of glaucoma. |
| first_indexed | 2024-04-12T14:49:59Z |
| format | Article |
| id | doaj.art-b4246dc66479473e83448425630ba185 |
| institution | Directory Open Access Journal |
| issn | 2222-3959 2227-4898 |
| language | English |
| last_indexed | 2024-04-12T14:49:59Z |
| publishDate | 2015-08-01 |
| publisher | Press of International Journal of Ophthalmology (IJO PRESS) |
| record_format | Article |
| series | International Journal of Ophthalmology |
| spelling | doaj.art-b4246dc66479473e83448425630ba1852022-12-22T03:28:30ZengPress of International Journal of Ophthalmology (IJO PRESS)International Journal of Ophthalmology2222-39592227-48982015-08-018464365210.3980/j.issn.2222-3959.2015.04.01β-Ⅲ-Tubulin:a reliable marker for retinal ganglion cell labeling in experimental models of glaucomaShan-Ming Jiang0Li-Ping Zeng1Ji-Hong Zeng2Li Tang3Xiao-Ming Chen4Xin Wei5Department of Ophthalmology, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, ChinaDepartment of Ophthalmology, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, ChinaDepartment of Ophthalmology, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, ChinaDepartment of Ophthalmology, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, ChinaDepartment of Ophthalmology, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, ChinaDepartment of Ophthalmology, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China<b>AIM:</b> To evaluate the reliability of β-III-Tubulin protein as a retinal ganglion cell (RGC) marker in the experimental glaucoma model. <b>METHODS:</b> Glaucoma mouse models were established by injecting polystyrene microbeads into the anterior chamber of C57BL/6J mice, then their retinas were obtained 14d and 28d after the intraocular pressure (IOP) was elevated. Retinal flat mounts and sections were double-labeled by fluorogold (FG) and β-III-Tubulin antibody or single-labeled by β-III-Tubulin antibody, then RGCs were counted and compared respectively. <b>RESULTS:</b> IOP of the injected eyes were elevated significantly and reached the peak at 22.8±0.7 mm Hg by day 14 after injection, then dropped to 11.3±0.7 mm Hg by day 28. RGC numbers counted by FG labeling and β-III-Tubulin antibody labeling were 64 807±4930 and 64614±5054 respectively in the control group, with no significant difference. By day 14, RGCs in the experimental group decreased significantly compared to the control group, but there was no significant difference between the FG labeling counting and the β-III-Tubulin antibody labeling counting either in the experimental group or in the control group. The result was similar by day 28, with further RGC loss. <b>CONCLUSION:</b> Our result suggested that the β-III-Tubulin protein was not affected by IOP elevation and can be used as a reliable marker for RGC in experimental models of glaucoma.http://www.ijo.cn/en_publish/2015/4/20150401.pdfβ-III-Tubulinglaucomafluorogoldretinal ganglion cell |
| spellingShingle | Shan-Ming Jiang Li-Ping Zeng Ji-Hong Zeng Li Tang Xiao-Ming Chen Xin Wei β-Ⅲ-Tubulin:a reliable marker for retinal ganglion cell labeling in experimental models of glaucoma International Journal of Ophthalmology β-III-Tubulin glaucoma fluorogold retinal ganglion cell |
| title | β-Ⅲ-Tubulin:a reliable marker for retinal ganglion cell labeling in experimental models of glaucoma |
| title_full | β-Ⅲ-Tubulin:a reliable marker for retinal ganglion cell labeling in experimental models of glaucoma |
| title_fullStr | β-Ⅲ-Tubulin:a reliable marker for retinal ganglion cell labeling in experimental models of glaucoma |
| title_full_unstemmed | β-Ⅲ-Tubulin:a reliable marker for retinal ganglion cell labeling in experimental models of glaucoma |
| title_short | β-Ⅲ-Tubulin:a reliable marker for retinal ganglion cell labeling in experimental models of glaucoma |
| title_sort | β iii tubulin a reliable marker for retinal ganglion cell labeling in experimental models of glaucoma |
| topic | β-III-Tubulin glaucoma fluorogold retinal ganglion cell |
| url | http://www.ijo.cn/en_publish/2015/4/20150401.pdf |
| work_keys_str_mv | AT shanmingjiang biiitubulinareliablemarkerforretinalganglioncelllabelinginexperimentalmodelsofglaucoma AT lipingzeng biiitubulinareliablemarkerforretinalganglioncelllabelinginexperimentalmodelsofglaucoma AT jihongzeng biiitubulinareliablemarkerforretinalganglioncelllabelinginexperimentalmodelsofglaucoma AT litang biiitubulinareliablemarkerforretinalganglioncelllabelinginexperimentalmodelsofglaucoma AT xiaomingchen biiitubulinareliablemarkerforretinalganglioncelllabelinginexperimentalmodelsofglaucoma AT xinwei biiitubulinareliablemarkerforretinalganglioncelllabelinginexperimentalmodelsofglaucoma |