Identification and characterisation of a novel multidrug-resistant streptococcus, Streptococcus toyakuensis sp. nov., from a blood sample
ABSTRACT: Objectives: In 2018, we isolated multidrug-resistant α-haemolytic streptococci TP1632 from the blood of a 34-year-old patient with bacteraemia. This study aimed to characterise α-haemolytic streptococci TP1632 and elucidate its multidrug resistance mechanisms. Methods: TP1632 was characte...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-06-01
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| Series: | Journal of Global Antimicrobial Resistance |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213716522000972 |
| _version_ | 1817969670463946752 |
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| author | Takeaki Wajima Atsuya Hagimoto Emi Tanaka Yoshiaki Kawamura Hidemasa Nakaminami |
| author_facet | Takeaki Wajima Atsuya Hagimoto Emi Tanaka Yoshiaki Kawamura Hidemasa Nakaminami |
| author_sort | Takeaki Wajima |
| collection | DOAJ |
| description | ABSTRACT: Objectives: In 2018, we isolated multidrug-resistant α-haemolytic streptococci TP1632 from the blood of a 34-year-old patient with bacteraemia. This study aimed to characterise α-haemolytic streptococci TP1632 and elucidate its multidrug resistance mechanisms. Methods: TP1632 was characterised by whole genome sequencing and biochemical testing. Based on the genome sequence, digital DNA-DNA hybridization (dDDH) and average nucleotide identity based on BLAST (ANIb) values were calculated. In addition, antimicrobial-resistance mechanisms were evaluated by ResFinder and transformation assay using Streptococcus mitis. Results: TP1632 showed resistance to β-lactams, macrolides, and quinolones. Genomic analysis revealed that dDDH and ANIb values between TP1632 and S. mitis were 56.3% and 93.63%, respectively, indicating TP1632 as the novel species. TP1632 exhibited macrolide resistance genes such as mef(A), msr(D), and erm(B) and tetracycline resistance gene tet(M). In addition, amino acids at position 81 in GyrA and 79 in ParC were tyrosine and isoleucine, respectively. When penicillin-binding proteins of TP1632 were introduced into S. mitis, recombinants showed β-lactam resistance. Thus, acquired resistance genes and low affinities towards antimicrobial agents contribute to multidrug resistance. Conclusion: Our findings indicated that multidrug-resistant TP1632 is a novel species in the genus Streptococcus. Furthermore, antimicrobial resistance of this strain could transfer horizontally among α-haemolytic streptococci. These data indicated the risk of emergence of multidrug-resistant streptococci not only as a pathogen but also a reservoir of antimicrobial resistance. This isolate was proposed as a novel species, Streptococcus toyakuensis sp. nov. The type strain is TP1632T (= JCM 34623T = CCUG 75492T). |
| first_indexed | 2024-04-13T20:24:05Z |
| format | Article |
| id | doaj.art-b4264e91942a41a6a3bd1525f22c8d55 |
| institution | Directory Open Access Journal |
| issn | 2213-7165 |
| language | English |
| last_indexed | 2024-04-13T20:24:05Z |
| publishDate | 2022-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Global Antimicrobial Resistance |
| spelling | doaj.art-b4264e91942a41a6a3bd1525f22c8d552022-12-22T02:31:25ZengElsevierJournal of Global Antimicrobial Resistance2213-71652022-06-0129316322Identification and characterisation of a novel multidrug-resistant streptococcus, Streptococcus toyakuensis sp. nov., from a blood sampleTakeaki Wajima0Atsuya Hagimoto1Emi Tanaka2Yoshiaki Kawamura3Hidemasa Nakaminami4Department of Clinical Microbiology, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Tokyo, Japan; Department of Microbiology, Faculty of Pharmacy, Meijo University, Nagoya, Japan; Corresponding author. Meijo University, 150 Yagotoyama, Tenpaku, Nagoya 468-8503, Japan.Department of Clinical Microbiology, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Tokyo, JapanDepartment of Clinical Microbiology, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Tokyo, Japan; Department of Microbiology, Faculty of Pharmacy, Meijo University, Nagoya, JapanLaboratory of Microbiology, School of Pharmacy, Aichi Gakuin University, Nagoya, JapanDepartment of Clinical Microbiology, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Tokyo, JapanABSTRACT: Objectives: In 2018, we isolated multidrug-resistant α-haemolytic streptococci TP1632 from the blood of a 34-year-old patient with bacteraemia. This study aimed to characterise α-haemolytic streptococci TP1632 and elucidate its multidrug resistance mechanisms. Methods: TP1632 was characterised by whole genome sequencing and biochemical testing. Based on the genome sequence, digital DNA-DNA hybridization (dDDH) and average nucleotide identity based on BLAST (ANIb) values were calculated. In addition, antimicrobial-resistance mechanisms were evaluated by ResFinder and transformation assay using Streptococcus mitis. Results: TP1632 showed resistance to β-lactams, macrolides, and quinolones. Genomic analysis revealed that dDDH and ANIb values between TP1632 and S. mitis were 56.3% and 93.63%, respectively, indicating TP1632 as the novel species. TP1632 exhibited macrolide resistance genes such as mef(A), msr(D), and erm(B) and tetracycline resistance gene tet(M). In addition, amino acids at position 81 in GyrA and 79 in ParC were tyrosine and isoleucine, respectively. When penicillin-binding proteins of TP1632 were introduced into S. mitis, recombinants showed β-lactam resistance. Thus, acquired resistance genes and low affinities towards antimicrobial agents contribute to multidrug resistance. Conclusion: Our findings indicated that multidrug-resistant TP1632 is a novel species in the genus Streptococcus. Furthermore, antimicrobial resistance of this strain could transfer horizontally among α-haemolytic streptococci. These data indicated the risk of emergence of multidrug-resistant streptococci not only as a pathogen but also a reservoir of antimicrobial resistance. This isolate was proposed as a novel species, Streptococcus toyakuensis sp. nov. The type strain is TP1632T (= JCM 34623T = CCUG 75492T).http://www.sciencedirect.com/science/article/pii/S2213716522000972Viridans streptococciMultidrug resistanceMultipleBacterialStreptococcus |
| spellingShingle | Takeaki Wajima Atsuya Hagimoto Emi Tanaka Yoshiaki Kawamura Hidemasa Nakaminami Identification and characterisation of a novel multidrug-resistant streptococcus, Streptococcus toyakuensis sp. nov., from a blood sample Journal of Global Antimicrobial Resistance Viridans streptococci Multidrug resistance Multiple Bacterial Streptococcus |
| title | Identification and characterisation of a novel multidrug-resistant streptococcus, Streptococcus toyakuensis sp. nov., from a blood sample |
| title_full | Identification and characterisation of a novel multidrug-resistant streptococcus, Streptococcus toyakuensis sp. nov., from a blood sample |
| title_fullStr | Identification and characterisation of a novel multidrug-resistant streptococcus, Streptococcus toyakuensis sp. nov., from a blood sample |
| title_full_unstemmed | Identification and characterisation of a novel multidrug-resistant streptococcus, Streptococcus toyakuensis sp. nov., from a blood sample |
| title_short | Identification and characterisation of a novel multidrug-resistant streptococcus, Streptococcus toyakuensis sp. nov., from a blood sample |
| title_sort | identification and characterisation of a novel multidrug resistant streptococcus streptococcus toyakuensis sp nov from a blood sample |
| topic | Viridans streptococci Multidrug resistance Multiple Bacterial Streptococcus |
| url | http://www.sciencedirect.com/science/article/pii/S2213716522000972 |
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