Genome-Wide Identification of Neuropeptides and Their Receptors in an Aphid Endoparasitoid Wasp, <i>Aphidius gifuensi</i>
In insects, neuropeptides and their receptors not only play a critical role in insect physiology and behavior but also are the potential targets for novel pesticide discoveries. <i>Aphidius gifuensis</i> is one of the most important and widespread aphid parasitoids, and has been successf...
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MDPI AG
2021-08-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/12/8/745 |
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| author | Xue Kong Zhen-Xiang Li Yu-Qing Gao Fang-Hua Liu Zhen-Zhen Chen Hong-Gang Tian Tong-Xian Liu Yong-Yu Xu Zhi-Wei Kang |
| author_facet | Xue Kong Zhen-Xiang Li Yu-Qing Gao Fang-Hua Liu Zhen-Zhen Chen Hong-Gang Tian Tong-Xian Liu Yong-Yu Xu Zhi-Wei Kang |
| author_sort | Xue Kong |
| collection | DOAJ |
| description | In insects, neuropeptides and their receptors not only play a critical role in insect physiology and behavior but also are the potential targets for novel pesticide discoveries. <i>Aphidius gifuensis</i> is one of the most important and widespread aphid parasitoids, and has been successfully used to control aphid. In the present work, we systematically identified neuropeptides and their receptors from the genome and head transcriptome of <i>A. gifuensis</i>. A total of 35 neuropeptide precursors and 49 corresponding receptors were identified. The phylogenetic analyses demonstrated that 35 of these receptors belong to family-A, four belong to family-B, two belong to leucine-rich repeat-containing GPCRs, four belong to receptor guanylyl cyclases, and four belong to receptor tyrosine kinases. Oral ingestion of imidacloprid significantly up-regulated five neuropeptide precursors and four receptors whereas three neuropeptide precursors and eight receptors were significantly down-regulated, which indicated that these neuropeptides and their receptors are potential targets of some commercial insecticides. The RT-qPCR results showed that dopamine receptor 1, dopamine receptor 2, octopamine receptor, allatostatin-A receptor, neuropeptides capa receptor, SIFamide receptor, FMRFamide receptor, tyramine receptor and short neuropeptide F predominantly were expressed in the head whilst the expression of ion transport peptide showed widespread distribution in various tissues. The high expression levels of these genes suggest their important roles in the central nervous system. Taken together, our study provides fundamental information that may further our understanding of neuropeptidergic signaling systems in the regulation of the physiology and behavior of solitary wasps. Furthermore, this information could also aid in the design and discovery of specific and environment-friendly insecticides. |
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| last_indexed | 2024-03-10T08:43:13Z |
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| series | Insects |
| spelling | doaj.art-b426edea58ce4bc9bc4fec075a7c38df2023-11-22T08:07:55ZengMDPI AGInsects2075-44502021-08-0112874510.3390/insects12080745Genome-Wide Identification of Neuropeptides and Their Receptors in an Aphid Endoparasitoid Wasp, <i>Aphidius gifuensi</i>Xue Kong0Zhen-Xiang Li1Yu-Qing Gao2Fang-Hua Liu3Zhen-Zhen Chen4Hong-Gang Tian5Tong-Xian Liu6Yong-Yu Xu7Zhi-Wei Kang8College of Plant Protection, Shandong Agricultural University, Tai’an 271018, ChinaCollege of Plant Protection, Shandong Agricultural University, Tai’an 271018, ChinaCollege of Plant Protection, Shandong Agricultural University, Tai’an 271018, ChinaCollege of Plant Protection, Shandong Agricultural University, Tai’an 271018, ChinaCollege of Plant Protection, Shandong Agricultural University, Tai’an 271018, ChinaState Key Laboratory of Crop Stress Biology for the Arid Areas, Key Laboratory of Northwest Loess Plateau Crop Pest Management of Ministry of Agriculture, Northwest A&F University, Yangling 712100, ChinaKey Laboratory of Integrated Crop Pest Management of Shandong Province, College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao 266109, ChinaCollege of Plant Protection, Shandong Agricultural University, Tai’an 271018, ChinaCollege of Plant Protection, Shandong Agricultural University, Tai’an 271018, ChinaIn insects, neuropeptides and their receptors not only play a critical role in insect physiology and behavior but also are the potential targets for novel pesticide discoveries. <i>Aphidius gifuensis</i> is one of the most important and widespread aphid parasitoids, and has been successfully used to control aphid. In the present work, we systematically identified neuropeptides and their receptors from the genome and head transcriptome of <i>A. gifuensis</i>. A total of 35 neuropeptide precursors and 49 corresponding receptors were identified. The phylogenetic analyses demonstrated that 35 of these receptors belong to family-A, four belong to family-B, two belong to leucine-rich repeat-containing GPCRs, four belong to receptor guanylyl cyclases, and four belong to receptor tyrosine kinases. Oral ingestion of imidacloprid significantly up-regulated five neuropeptide precursors and four receptors whereas three neuropeptide precursors and eight receptors were significantly down-regulated, which indicated that these neuropeptides and their receptors are potential targets of some commercial insecticides. The RT-qPCR results showed that dopamine receptor 1, dopamine receptor 2, octopamine receptor, allatostatin-A receptor, neuropeptides capa receptor, SIFamide receptor, FMRFamide receptor, tyramine receptor and short neuropeptide F predominantly were expressed in the head whilst the expression of ion transport peptide showed widespread distribution in various tissues. The high expression levels of these genes suggest their important roles in the central nervous system. Taken together, our study provides fundamental information that may further our understanding of neuropeptidergic signaling systems in the regulation of the physiology and behavior of solitary wasps. Furthermore, this information could also aid in the design and discovery of specific and environment-friendly insecticides.https://www.mdpi.com/2075-4450/12/8/745<i>Aphidius gifuensis</i>neuropeptide precursorsneuropeptide receptorsexpression profile |
| spellingShingle | Xue Kong Zhen-Xiang Li Yu-Qing Gao Fang-Hua Liu Zhen-Zhen Chen Hong-Gang Tian Tong-Xian Liu Yong-Yu Xu Zhi-Wei Kang Genome-Wide Identification of Neuropeptides and Their Receptors in an Aphid Endoparasitoid Wasp, <i>Aphidius gifuensi</i> Insects <i>Aphidius gifuensis</i> neuropeptide precursors neuropeptide receptors expression profile |
| title | Genome-Wide Identification of Neuropeptides and Their Receptors in an Aphid Endoparasitoid Wasp, <i>Aphidius gifuensi</i> |
| title_full | Genome-Wide Identification of Neuropeptides and Their Receptors in an Aphid Endoparasitoid Wasp, <i>Aphidius gifuensi</i> |
| title_fullStr | Genome-Wide Identification of Neuropeptides and Their Receptors in an Aphid Endoparasitoid Wasp, <i>Aphidius gifuensi</i> |
| title_full_unstemmed | Genome-Wide Identification of Neuropeptides and Their Receptors in an Aphid Endoparasitoid Wasp, <i>Aphidius gifuensi</i> |
| title_short | Genome-Wide Identification of Neuropeptides and Their Receptors in an Aphid Endoparasitoid Wasp, <i>Aphidius gifuensi</i> |
| title_sort | genome wide identification of neuropeptides and their receptors in an aphid endoparasitoid wasp i aphidius gifuensi i |
| topic | <i>Aphidius gifuensis</i> neuropeptide precursors neuropeptide receptors expression profile |
| url | https://www.mdpi.com/2075-4450/12/8/745 |
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