Detection of Metallo-beta-lactamase blaVIM-1 Gene in Clinical Isolates of Pseudomonas Aeruginosa in Mashhad
Introduction: Carbapenems are one of the latest drugs for treatment of threatening infections of Pseudomonas aeruginosa. Resistance to these antibiotics is sometimes caused by carbapnmase class B, such as VIM metallo-beta-lactamase (MBL) that is growing in the communities. The aim of this study was...
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| Format: | Article |
| Language: | fas |
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Shahid Sadoughi University of Medical Sciences
2016-04-01
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| Series: | Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Shahīd Ṣadūqī Yazd |
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| Online Access: | http://jssu.ssu.ac.ir/browse.php?a_code=A-10-2382-1&slc_lang=en&sid=1 |
| _version_ | 1819207539129057280 |
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| author | M Nakhaei Moghaddam M Hosseini Hasanabady |
| author_facet | M Nakhaei Moghaddam M Hosseini Hasanabady |
| author_sort | M Nakhaei Moghaddam |
| collection | DOAJ |
| description | Introduction: Carbapenems are one of the latest drugs for treatment of threatening infections of Pseudomonas aeruginosa. Resistance to these antibiotics is sometimes caused by carbapnmase class B, such as VIM metallo-beta-lactamase (MBL) that is growing in the communities. The aim of this study was to detect the of metallo-beta-lactamase blaVIM-1 gene among clinical isolates of Pseudomonas aeruginosa in Mashhad.
Methods: In this cross-sectional study, 70 isolates of Pseudomonas aeruginosa were collected from samples of inpatients at hospitals in Mashhad. Bacteria were identified by conventional biochemical methods. Antibiotic susceptibility testing was examined in accordance with the Kirby-Bauer method. Metallo-beta-lactamase- producing isolates were identified using a combination disc of imipenem and EDTA. Then, polymerase chain reaction was performed using specific primers to detect blaVIM-1 gene.
Results: 25 (35.7%) out of 70 isolated bacteria had metallo-beta- lactamase, which blaVIM-1 gene were detected in the genome of 8 isolates. 65.6% of resistant bacteria to imipenem produced metallo-beta-lactamase (MBL). A higher percentage of MBL- producing isolates were resistant to imipenem, meropenem, carbenicillin and cefotaxime (p < 0.05).
Conclusion: The results of the study showed that antibiotic resistance among examined clinical isolates of Paeruginosa was high and VIM-type metallo-beta-lactamase was detected among them. Identification of bacteria with metallo-beta-lactamase (MBL) is very important in the prevention and treatment of infections resistant to antibiotics. |
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| format | Article |
| id | doaj.art-b42a1d5baf474da7a21eaf03ecfee80c |
| institution | Directory Open Access Journal |
| issn | 2228-5741 2228-5733 |
| language | fas |
| last_indexed | 2024-12-23T05:25:06Z |
| publishDate | 2016-04-01 |
| publisher | Shahid Sadoughi University of Medical Sciences |
| record_format | Article |
| series | Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Shahīd Ṣadūqī Yazd |
| spelling | doaj.art-b42a1d5baf474da7a21eaf03ecfee80c2022-12-21T17:58:37ZfasShahid Sadoughi University of Medical SciencesMajallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Shahīd Ṣadūqī Yazd2228-57412228-57332016-04-012417482Detection of Metallo-beta-lactamase blaVIM-1 Gene in Clinical Isolates of Pseudomonas Aeruginosa in MashhadM Nakhaei Moghaddam0M Hosseini Hasanabady1 Islamic Azad University, Mashhad Islamic Azad University, Mashhad Introduction: Carbapenems are one of the latest drugs for treatment of threatening infections of Pseudomonas aeruginosa. Resistance to these antibiotics is sometimes caused by carbapnmase class B, such as VIM metallo-beta-lactamase (MBL) that is growing in the communities. The aim of this study was to detect the of metallo-beta-lactamase blaVIM-1 gene among clinical isolates of Pseudomonas aeruginosa in Mashhad. Methods: In this cross-sectional study, 70 isolates of Pseudomonas aeruginosa were collected from samples of inpatients at hospitals in Mashhad. Bacteria were identified by conventional biochemical methods. Antibiotic susceptibility testing was examined in accordance with the Kirby-Bauer method. Metallo-beta-lactamase- producing isolates were identified using a combination disc of imipenem and EDTA. Then, polymerase chain reaction was performed using specific primers to detect blaVIM-1 gene. Results: 25 (35.7%) out of 70 isolated bacteria had metallo-beta- lactamase, which blaVIM-1 gene were detected in the genome of 8 isolates. 65.6% of resistant bacteria to imipenem produced metallo-beta-lactamase (MBL). A higher percentage of MBL- producing isolates were resistant to imipenem, meropenem, carbenicillin and cefotaxime (p < 0.05). Conclusion: The results of the study showed that antibiotic resistance among examined clinical isolates of Paeruginosa was high and VIM-type metallo-beta-lactamase was detected among them. Identification of bacteria with metallo-beta-lactamase (MBL) is very important in the prevention and treatment of infections resistant to antibiotics.http://jssu.ssu.ac.ir/browse.php?a_code=A-10-2382-1&slc_lang=en&sid=1Pseudomonas Aeruginosa Metallo-beta-lactamase blaVIM-1 Gene Antibiotic Resistance |
| spellingShingle | M Nakhaei Moghaddam M Hosseini Hasanabady Detection of Metallo-beta-lactamase blaVIM-1 Gene in Clinical Isolates of Pseudomonas Aeruginosa in Mashhad Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Shahīd Ṣadūqī Yazd Pseudomonas Aeruginosa Metallo-beta-lactamase blaVIM-1 Gene Antibiotic Resistance |
| title | Detection of Metallo-beta-lactamase blaVIM-1 Gene in Clinical Isolates of Pseudomonas Aeruginosa in Mashhad |
| title_full | Detection of Metallo-beta-lactamase blaVIM-1 Gene in Clinical Isolates of Pseudomonas Aeruginosa in Mashhad |
| title_fullStr | Detection of Metallo-beta-lactamase blaVIM-1 Gene in Clinical Isolates of Pseudomonas Aeruginosa in Mashhad |
| title_full_unstemmed | Detection of Metallo-beta-lactamase blaVIM-1 Gene in Clinical Isolates of Pseudomonas Aeruginosa in Mashhad |
| title_short | Detection of Metallo-beta-lactamase blaVIM-1 Gene in Clinical Isolates of Pseudomonas Aeruginosa in Mashhad |
| title_sort | detection of metallo beta lactamase blavim 1 gene in clinical isolates of pseudomonas aeruginosa in mashhad |
| topic | Pseudomonas Aeruginosa Metallo-beta-lactamase blaVIM-1 Gene Antibiotic Resistance |
| url | http://jssu.ssu.ac.ir/browse.php?a_code=A-10-2382-1&slc_lang=en&sid=1 |
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