Ribosome inactivation by Escherichia coli GTPase RsgA inhibits T4 phage
IntroductionBacteria must combat phages, and myriad bacterial anti-phage systems have been discovered that reduce host metabolism, for example, by depleting energetic compounds like ATP and NAD+. Hence, these systems indirectly inhibit protein production. Surprisingly, direct reduction of ribosome a...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-08-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1242163/full |
| _version_ | 1797740124489908224 |
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| author | Laura Fernández-García Laura Fernández-García María Tomás Thomas K. Wood |
| author_facet | Laura Fernández-García Laura Fernández-García María Tomás Thomas K. Wood |
| author_sort | Laura Fernández-García |
| collection | DOAJ |
| description | IntroductionBacteria must combat phages, and myriad bacterial anti-phage systems have been discovered that reduce host metabolism, for example, by depleting energetic compounds like ATP and NAD+. Hence, these systems indirectly inhibit protein production. Surprisingly, direct reduction of ribosome activity has not been demonstrated to thwart phage.MethodsHere, by producing each of the 4,287 Escherichia coli proteins and selecting for anti-phage activity that leads to enhanced growth, we investigated the role of host proteins in phage inhibition.Results and discussionWe identified that E. coli GTPase RsgA inhibits lytic phage T4 by inactivating ribosomes. |
| first_indexed | 2024-03-12T14:08:02Z |
| format | Article |
| id | doaj.art-b432ccff071d485aa60435781cc38ff7 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-12T14:08:02Z |
| publishDate | 2023-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-b432ccff071d485aa60435781cc38ff72023-08-21T12:38:41ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-08-011410.3389/fmicb.2023.12421631242163Ribosome inactivation by Escherichia coli GTPase RsgA inhibits T4 phageLaura Fernández-García0Laura Fernández-García1María Tomás2Thomas K. Wood3Department of Chemical Engineering, Pennsylvania State University, University Park, PA, United StatesMicrobiology Translational and Multidisciplinary (MicroTM)-Research Institute Biomedical A Coruña (INIBIC) and Microbiology Department of Hospital A Coruña (CHUAC), University of A Coruña (UDC), A Coruña, SpainMicrobiology Translational and Multidisciplinary (MicroTM)-Research Institute Biomedical A Coruña (INIBIC) and Microbiology Department of Hospital A Coruña (CHUAC), University of A Coruña (UDC), A Coruña, SpainDepartment of Chemical Engineering, Pennsylvania State University, University Park, PA, United StatesIntroductionBacteria must combat phages, and myriad bacterial anti-phage systems have been discovered that reduce host metabolism, for example, by depleting energetic compounds like ATP and NAD+. Hence, these systems indirectly inhibit protein production. Surprisingly, direct reduction of ribosome activity has not been demonstrated to thwart phage.MethodsHere, by producing each of the 4,287 Escherichia coli proteins and selecting for anti-phage activity that leads to enhanced growth, we investigated the role of host proteins in phage inhibition.Results and discussionWe identified that E. coli GTPase RsgA inhibits lytic phage T4 by inactivating ribosomes.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1242163/fullRsgAphage inhibitionribosome inactivationpersistenceribosomes |
| spellingShingle | Laura Fernández-García Laura Fernández-García María Tomás Thomas K. Wood Ribosome inactivation by Escherichia coli GTPase RsgA inhibits T4 phage Frontiers in Microbiology RsgA phage inhibition ribosome inactivation persistence ribosomes |
| title | Ribosome inactivation by Escherichia coli GTPase RsgA inhibits T4 phage |
| title_full | Ribosome inactivation by Escherichia coli GTPase RsgA inhibits T4 phage |
| title_fullStr | Ribosome inactivation by Escherichia coli GTPase RsgA inhibits T4 phage |
| title_full_unstemmed | Ribosome inactivation by Escherichia coli GTPase RsgA inhibits T4 phage |
| title_short | Ribosome inactivation by Escherichia coli GTPase RsgA inhibits T4 phage |
| title_sort | ribosome inactivation by escherichia coli gtpase rsga inhibits t4 phage |
| topic | RsgA phage inhibition ribosome inactivation persistence ribosomes |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1242163/full |
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