Establishment of a Zebrafish Xenograft Model for Investigation of Nasopharyngeal Carcinoma
Nasopharyngeal carcinoma (NPC) is a unique malignant tumor of the head and neck. Despite higher survival rates by the combination of radiotherapy and chemotherapy, the recurrence or metastasis of NPC still occurs at about 10%. Therefore, there is urgent demand to develop more effective in vivo model...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2022-09-01
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| Series: | Cell Transplantation |
| Online Access: | https://doi.org/10.1177/09636897221116085 |
| _version_ | 1811260122491518976 |
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| author | Enyu Huang Haofeng Huang Longji Wu Binbin Li Zhiwei He Jingjing Zhang |
| author_facet | Enyu Huang Haofeng Huang Longji Wu Binbin Li Zhiwei He Jingjing Zhang |
| author_sort | Enyu Huang |
| collection | DOAJ |
| description | Nasopharyngeal carcinoma (NPC) is a unique malignant tumor of the head and neck. Despite higher survival rates by the combination of radiotherapy and chemotherapy, the recurrence or metastasis of NPC still occurs at about 10%. Therefore, there is urgent demand to develop more effective in vivo models for preclinical trials to investigate the mechanisms of NPC development and progression and to explore better treatment approaches. In this study, we transplanted human NPC CNE1 cells into zebrafish embryos to establish a xenograft model of NPC, where the proliferation and invasion behaviors of NPC cells were investigated in vivo . Combining in vitro and in vivo analyses, we found that activating transcription factor 7 (ATF7) was involved in the occurrence and development of NPC regulated by peptidyl-prolyl cis - trans isomerase NIMA-interacting 1 (Pin1). The zebrafish NPC xenograft model established here thereby provides an in vivo tool for exploring the occurrence and development of NPC, which may help to identify new tumor markers and develop new therapeutic strategies for the treatment of NPC. |
| first_indexed | 2024-04-12T18:41:48Z |
| format | Article |
| id | doaj.art-b4344353f2b344838f323b4ad75cf541 |
| institution | Directory Open Access Journal |
| issn | 1555-3892 |
| language | English |
| last_indexed | 2024-04-12T18:41:48Z |
| publishDate | 2022-09-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Cell Transplantation |
| spelling | doaj.art-b4344353f2b344838f323b4ad75cf5412022-12-22T03:20:45ZengSAGE PublishingCell Transplantation1555-38922022-09-013110.1177/09636897221116085Establishment of a Zebrafish Xenograft Model for Investigation of Nasopharyngeal CarcinomaEnyu Huang0Haofeng Huang1Longji Wu2Binbin Li3Zhiwei He4Jingjing Zhang5China-American Cancer Research Institute, Guangdong Medical University, Dongguan, ChinaAffiliated Hospital of Guangdong Medical University & Key Laboratory of Zebrafish Model for Development and Disease of Guangdong Medical University, Zhanjiang, ChinaChina-American Cancer Research Institute, Guangdong Medical University, Dongguan, ChinaChina-American Cancer Research Institute, Guangdong Medical University, Dongguan, ChinaChina-American Cancer Research Institute, Guangdong Medical University, Dongguan, ChinaThe Marine Biomedical Research Institute of Guangdong Zhanjiang, Zhanjiang, ChinaNasopharyngeal carcinoma (NPC) is a unique malignant tumor of the head and neck. Despite higher survival rates by the combination of radiotherapy and chemotherapy, the recurrence or metastasis of NPC still occurs at about 10%. Therefore, there is urgent demand to develop more effective in vivo models for preclinical trials to investigate the mechanisms of NPC development and progression and to explore better treatment approaches. In this study, we transplanted human NPC CNE1 cells into zebrafish embryos to establish a xenograft model of NPC, where the proliferation and invasion behaviors of NPC cells were investigated in vivo . Combining in vitro and in vivo analyses, we found that activating transcription factor 7 (ATF7) was involved in the occurrence and development of NPC regulated by peptidyl-prolyl cis - trans isomerase NIMA-interacting 1 (Pin1). The zebrafish NPC xenograft model established here thereby provides an in vivo tool for exploring the occurrence and development of NPC, which may help to identify new tumor markers and develop new therapeutic strategies for the treatment of NPC.https://doi.org/10.1177/09636897221116085 |
| spellingShingle | Enyu Huang Haofeng Huang Longji Wu Binbin Li Zhiwei He Jingjing Zhang Establishment of a Zebrafish Xenograft Model for Investigation of Nasopharyngeal Carcinoma Cell Transplantation |
| title | Establishment of a Zebrafish Xenograft Model for Investigation of Nasopharyngeal Carcinoma |
| title_full | Establishment of a Zebrafish Xenograft Model for Investigation of Nasopharyngeal Carcinoma |
| title_fullStr | Establishment of a Zebrafish Xenograft Model for Investigation of Nasopharyngeal Carcinoma |
| title_full_unstemmed | Establishment of a Zebrafish Xenograft Model for Investigation of Nasopharyngeal Carcinoma |
| title_short | Establishment of a Zebrafish Xenograft Model for Investigation of Nasopharyngeal Carcinoma |
| title_sort | establishment of a zebrafish xenograft model for investigation of nasopharyngeal carcinoma |
| url | https://doi.org/10.1177/09636897221116085 |
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