Thermo-sensitive hydrogel combined with SHH expressed RMSCs for rat spinal cord regeneration
Purpose: Spinal cord injury (SCI) has a damaging impact on patients, amid being a worldwide problem with no effective treatment. Herein, we reported a method for functional therapy of SCI in rats, wherein we combined thermo-sensitive hydrogel with Sonic Hedgehog (SHH) expressed in rat bone-marrow de...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2022.1001396/full |
| _version_ | 1798028834292891648 |
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| author | Jun Gu Jun Gu Biao Gao Biao Gao Hajra Zafar Bo Chu Xiaojun Feng Yinjie Ni Lin Xu Rui Bao |
| author_facet | Jun Gu Jun Gu Biao Gao Biao Gao Hajra Zafar Bo Chu Xiaojun Feng Yinjie Ni Lin Xu Rui Bao |
| author_sort | Jun Gu |
| collection | DOAJ |
| description | Purpose: Spinal cord injury (SCI) has a damaging impact on patients, amid being a worldwide problem with no effective treatment. Herein, we reported a method for functional therapy of SCI in rats, wherein we combined thermo-sensitive hydrogel with Sonic Hedgehog (SHH) expressed in rat bone-marrow derived mesenchymal stem cells (RMSCs).Methods: Bone marrow-derived mesenchymal stem cells (BMSCs) were isolated from Sprague-Dawley (SD) female rats. The SHH was optimized and transferred into RMSCs via cationic liposomes, while thermo-sensitive hydrogel was reformed with hyaluronate (HA) and Pluronic F127. Then, a rat model with SCI was established accordingly by male SD rats and randomized into sham, model, RMSCs with hydrogel and SHH-RMSCs with hydrogel. The evaluation of SCI repair based on Basso, Beattie Bresnahanlocomotor rating scale (BBB scale) and inclined plate score. Immunofluorescence, immunohistochemistry and hematoxylin-eosin were utilized to explore the expression of protein (GFAP, GAP43, NF200 and MBP) and histopathology.Results: It was demonstrated that transfection of SHH with cationic liposomes exhibited more effect in RMSCs than lipofectamine 2000. As shown in SEM, 3.5% HA-F127 demonstrated porous structure. In the MTT and dead/live assay, 3.5% HA-F127 showed good biocompatibility for RMSCs. Both RMSCs and SHH-RMSCs groups could significantly promote BBB and inclined plate scores (p < 0.01) compared with the model. Furthermore, the SHH-RMSC group was significantly improved than RMSC with the expression of related proteins, where NF200, MBP, and GAP43 were principally enhanced with the GFAP expression being virtually down-regulated.Conclusion: All in all, the results suggested that transplantation of RMSCs with SHH could improve the function of SCI and promote nerve regeneration. |
| first_indexed | 2024-04-11T19:14:32Z |
| format | Article |
| id | doaj.art-b436d72a14f0458e960239e69eb98947 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-04-11T19:14:32Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-b436d72a14f0458e960239e69eb989472022-12-22T04:07:27ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852022-10-011010.3389/fbioe.2022.10013961001396Thermo-sensitive hydrogel combined with SHH expressed RMSCs for rat spinal cord regenerationJun Gu0Jun Gu1Biao Gao2Biao Gao3Hajra Zafar4Bo Chu5Xiaojun Feng6Yinjie Ni7Lin Xu8Rui Bao9School of Medicine, Yangzhou University, Yangzhou, ChinaDepartment of Orthopedics, Xishan People’s Hospital, Wuxi, ChinaSchool of Medicine, Yangzhou University, Yangzhou, ChinaWuxi Xishan District Ehu Town Health Center, Wuxi, ChinaSchool of Pharmacy, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Orthopedics, Xishan People’s Hospital, Wuxi, ChinaDepartment of Orthopedics, Xishan People’s Hospital, Wuxi, ChinaDepartment of Orthopedics, Xishan People’s Hospital, Wuxi, ChinaDepartment of Orthopedics, Xishan People’s Hospital, Wuxi, ChinaDepartment of Pharmaceutics, School of Pharmacy, Jiangsu University, Zhenjiang, ChinaPurpose: Spinal cord injury (SCI) has a damaging impact on patients, amid being a worldwide problem with no effective treatment. Herein, we reported a method for functional therapy of SCI in rats, wherein we combined thermo-sensitive hydrogel with Sonic Hedgehog (SHH) expressed in rat bone-marrow derived mesenchymal stem cells (RMSCs).Methods: Bone marrow-derived mesenchymal stem cells (BMSCs) were isolated from Sprague-Dawley (SD) female rats. The SHH was optimized and transferred into RMSCs via cationic liposomes, while thermo-sensitive hydrogel was reformed with hyaluronate (HA) and Pluronic F127. Then, a rat model with SCI was established accordingly by male SD rats and randomized into sham, model, RMSCs with hydrogel and SHH-RMSCs with hydrogel. The evaluation of SCI repair based on Basso, Beattie Bresnahanlocomotor rating scale (BBB scale) and inclined plate score. Immunofluorescence, immunohistochemistry and hematoxylin-eosin were utilized to explore the expression of protein (GFAP, GAP43, NF200 and MBP) and histopathology.Results: It was demonstrated that transfection of SHH with cationic liposomes exhibited more effect in RMSCs than lipofectamine 2000. As shown in SEM, 3.5% HA-F127 demonstrated porous structure. In the MTT and dead/live assay, 3.5% HA-F127 showed good biocompatibility for RMSCs. Both RMSCs and SHH-RMSCs groups could significantly promote BBB and inclined plate scores (p < 0.01) compared with the model. Furthermore, the SHH-RMSC group was significantly improved than RMSC with the expression of related proteins, where NF200, MBP, and GAP43 were principally enhanced with the GFAP expression being virtually down-regulated.Conclusion: All in all, the results suggested that transplantation of RMSCs with SHH could improve the function of SCI and promote nerve regeneration.https://www.frontiersin.org/articles/10.3389/fbioe.2022.1001396/fullspinal cord injurythermo-sensitive hydrogelShhrat bone marrow mesenchymal stem cellsnerve regeneration |
| spellingShingle | Jun Gu Jun Gu Biao Gao Biao Gao Hajra Zafar Bo Chu Xiaojun Feng Yinjie Ni Lin Xu Rui Bao Thermo-sensitive hydrogel combined with SHH expressed RMSCs for rat spinal cord regeneration Frontiers in Bioengineering and Biotechnology spinal cord injury thermo-sensitive hydrogel Shh rat bone marrow mesenchymal stem cells nerve regeneration |
| title | Thermo-sensitive hydrogel combined with SHH expressed RMSCs for rat spinal cord regeneration |
| title_full | Thermo-sensitive hydrogel combined with SHH expressed RMSCs for rat spinal cord regeneration |
| title_fullStr | Thermo-sensitive hydrogel combined with SHH expressed RMSCs for rat spinal cord regeneration |
| title_full_unstemmed | Thermo-sensitive hydrogel combined with SHH expressed RMSCs for rat spinal cord regeneration |
| title_short | Thermo-sensitive hydrogel combined with SHH expressed RMSCs for rat spinal cord regeneration |
| title_sort | thermo sensitive hydrogel combined with shh expressed rmscs for rat spinal cord regeneration |
| topic | spinal cord injury thermo-sensitive hydrogel Shh rat bone marrow mesenchymal stem cells nerve regeneration |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2022.1001396/full |
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