Controlling Unconventional Secretion for Production of Heterologous Proteins in <i>Ustilago maydis</i> through Transcriptional Regulation and Chemical Inhibition of the Kinase Don3

Controlling Unconventional Secretion for Production of Heterologous Proteins in <i>Ustilago maydis</i> through Transcriptional Regulation and Chemical Inhibition of the Kinase Don3

Heterologous protein production is a highly demanded biotechnological process. Secretion of the product to the culture broth is advantageous because it drastically reduces downstream processing costs. We exploit unconventional secretion for heterologous protein expression in the fungal model microor...

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Bibliographic Details
Main Authors: Kai P. Hussnaetter, Magnus Philipp, Kira Müntjes, Michael Feldbrügge, Kerstin Schipper
Format: Article
Language:English
Published: MDPI AG 2021-03-01
Series:Journal of Fungi
Subjects:
autoinduction
chemical genetics
cytokinesis
inducible promoter
nanobody
regulated secretion
Online Access:https://www.mdpi.com/2309-608X/7/3/179

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