Screening and identification of LncRNAs targeting site binding of cathepsin D in chronic light-damaged skin cells
Objective: To investigate the role of LncRNAs that regulate cathepsin D in chronic light-damaged skin cells and explore their role in the mechanism of skin photoaging. Methods: Cultured human dermal fibroblasts were induced to a photoaging cells model by repetitive UVA radiation (UVA radiation group...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | zho |
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editoiral office of Journal of Diagnosis and Therapy on Dermato-venereology
2021-04-01
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| Series: | Pifu-xingbing zhenliaoxue zazhi |
| Subjects: | |
| Online Access: | http://pfxbzlx.gdvdc.com/CN/abstract/abstract1920.shtml |
| _version_ | 1811241975853088768 |
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| author | Weichun XU Wenyi HOU Wei LAI Yue ZHENG |
| author_facet | Weichun XU Wenyi HOU Wei LAI Yue ZHENG |
| author_sort | Weichun XU |
| collection | DOAJ |
| description | Objective: To investigate the role of LncRNAs that regulate cathepsin D in chronic light-damaged skin cells and explore their role in the mechanism of skin photoaging. Methods: Cultured human dermal fibroblasts were induced to a photoaging cells model by repetitive UVA radiation (UVA radiation group), which was evaluated by CCK8 assay, β-galactosidase staining and flow cytometry detection of apoptosis rate. High-throughput sequencing was used to detect LncRNA expression profiles. Functional annotation analysis was preformed to identify the LncRNAs that could regulate cathpeisn D. miRanda and TargetScan were preformed to analyze the miRNA that can target binding to LncRNA-cathpeisn D. Results: Compared with cells in the control group, the proliferative activity of cells in the UVA radiation group significantly decreased [(60.02±5.13)% vs (89.06±4.21)%, t=2.31, P<0.05], while the apoptosis rate of cells in the UVA radiation group significantly increased [(26.04±7.42)% vs (11.31±4.95)%, t=3.54, P<0.05], and the percentage of β-galactosidase of cells in the UVA radiation group significantly increased [(85.24±4.21)% vs (8.16±1.62)%, t=3.22, P<0.05]. Differential expression of LncRNAs was detected via high throughput sequencing technique. 25 differentially expressed LncRNAs were related to regulate cathepsin D including Lnc-CTSD, Lnc-TNNI1 and Lnc-RP11-706015.1.1-3 targeting to miR4298. Conclusion: Repeated UVA irradiation could up-regulate the expression of LncRNAs which regulate cathepsin D and all target to miR4298. The results in this study indicate that microRNA-LncRNA-cathepsin D may play an important role in skin photoaging. |
| first_indexed | 2024-04-12T13:44:05Z |
| format | Article |
| id | doaj.art-b440e38055ef4e1db69de722e5b67949 |
| institution | Directory Open Access Journal |
| issn | 1674-8468 |
| language | zho |
| last_indexed | 2024-04-12T13:44:05Z |
| publishDate | 2021-04-01 |
| publisher | editoiral office of Journal of Diagnosis and Therapy on Dermato-venereology |
| record_format | Article |
| series | Pifu-xingbing zhenliaoxue zazhi |
| spelling | doaj.art-b440e38055ef4e1db69de722e5b679492022-12-22T03:30:44Zzhoeditoiral office of Journal of Diagnosis and Therapy on Dermato-venereologyPifu-xingbing zhenliaoxue zazhi1674-84682021-04-01282818510.3969/j.issn.1674-8468.2021.02.001Screening and identification of LncRNAs targeting site binding of cathepsin D in chronic light-damaged skin cellsWeichun XU0Wenyi HOU1Wei LAI2Yue ZHENG3Department of Dermatology, The Third Affiliated Hospital, Yuedong Hospital, Sun Yat-sen University, Meizhou 514700, ChinaDepartment of Dermatology, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510620, ChinaDepartment of Dermatology, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510620, ChinaDepartment of Dermatology, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510620, ChinaObjective: To investigate the role of LncRNAs that regulate cathepsin D in chronic light-damaged skin cells and explore their role in the mechanism of skin photoaging. Methods: Cultured human dermal fibroblasts were induced to a photoaging cells model by repetitive UVA radiation (UVA radiation group), which was evaluated by CCK8 assay, β-galactosidase staining and flow cytometry detection of apoptosis rate. High-throughput sequencing was used to detect LncRNA expression profiles. Functional annotation analysis was preformed to identify the LncRNAs that could regulate cathpeisn D. miRanda and TargetScan were preformed to analyze the miRNA that can target binding to LncRNA-cathpeisn D. Results: Compared with cells in the control group, the proliferative activity of cells in the UVA radiation group significantly decreased [(60.02±5.13)% vs (89.06±4.21)%, t=2.31, P<0.05], while the apoptosis rate of cells in the UVA radiation group significantly increased [(26.04±7.42)% vs (11.31±4.95)%, t=3.54, P<0.05], and the percentage of β-galactosidase of cells in the UVA radiation group significantly increased [(85.24±4.21)% vs (8.16±1.62)%, t=3.22, P<0.05]. Differential expression of LncRNAs was detected via high throughput sequencing technique. 25 differentially expressed LncRNAs were related to regulate cathepsin D including Lnc-CTSD, Lnc-TNNI1 and Lnc-RP11-706015.1.1-3 targeting to miR4298. Conclusion: Repeated UVA irradiation could up-regulate the expression of LncRNAs which regulate cathepsin D and all target to miR4298. The results in this study indicate that microRNA-LncRNA-cathepsin D may play an important role in skin photoaging.http://pfxbzlx.gdvdc.com/CN/abstract/abstract1920.shtmlfibroblastultraviolet raycathepsin dlncrnasphotoagingmechanism |
| spellingShingle | Weichun XU Wenyi HOU Wei LAI Yue ZHENG Screening and identification of LncRNAs targeting site binding of cathepsin D in chronic light-damaged skin cells Pifu-xingbing zhenliaoxue zazhi fibroblast ultraviolet ray cathepsin d lncrnas photoaging mechanism |
| title | Screening and identification of LncRNAs targeting site binding of cathepsin D in chronic light-damaged skin cells |
| title_full | Screening and identification of LncRNAs targeting site binding of cathepsin D in chronic light-damaged skin cells |
| title_fullStr | Screening and identification of LncRNAs targeting site binding of cathepsin D in chronic light-damaged skin cells |
| title_full_unstemmed | Screening and identification of LncRNAs targeting site binding of cathepsin D in chronic light-damaged skin cells |
| title_short | Screening and identification of LncRNAs targeting site binding of cathepsin D in chronic light-damaged skin cells |
| title_sort | screening and identification of lncrnas targeting site binding of cathepsin d in chronic light damaged skin cells |
| topic | fibroblast ultraviolet ray cathepsin d lncrnas photoaging mechanism |
| url | http://pfxbzlx.gdvdc.com/CN/abstract/abstract1920.shtml |
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