Streptococcus mutans Membrane Vesicles Harboring Glucosyltransferases Augment Candida albicans Biofilm Development
Candida albicans, as the most common fungus in the oral cavity, is often detected in early childhood caries. Streptococcus mutans is the major etiological agent of dental caries, but the role of S. mutans on C. albicans growth and biofilm development remains to be elucidated. Membrane vesicles (MVs)...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-09-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2020.581184/full |
| _version_ | 1817977199052980224 |
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| author | Ruixue Wu Ruixue Wu Ye Tao Ye Tao Yina Cao Yina Cao Yan Zhou Yan Zhou Huancai Lin Huancai Lin |
| author_facet | Ruixue Wu Ruixue Wu Ye Tao Ye Tao Yina Cao Yina Cao Yan Zhou Yan Zhou Huancai Lin Huancai Lin |
| author_sort | Ruixue Wu |
| collection | DOAJ |
| description | Candida albicans, as the most common fungus in the oral cavity, is often detected in early childhood caries. Streptococcus mutans is the major etiological agent of dental caries, but the role of S. mutans on C. albicans growth and biofilm development remains to be elucidated. Membrane vesicles (MVs) are a cell-secreted subcellular fraction that play an important role in intercellular communication and disease progression. In the present study, we investigated whether MVs from S. mutans augment C. albicans growth and biofilm development. The results indicated that S. mutans MVs augmented C. albicans biofilm development but had no significant effect on C. albicans growth under planktonic conditions. Subsequently, we labeled S. mutans MVs with PKH26 and used confocal laser scanning microscopy (CLSM) to track S. mutans MVs, which were observed to be located in the C. albicans biofilm extracellular matrix. Monosaccharide tests showed that S. mutans MVs contribute to sucrose metabolism in C. albicans. Polysaccharides were significantly enriched in the S. mutans MV-treated group. MVs from ΔgtfBC mutant strains were compared with those from the wild-type S. mutans. The results revealed that MVs from the ΔgtfBC mutant had no effect on C. albicans biofilm formation and exopolysaccharide production. In addition, C. albicans biofilm transcriptional regulators (Ndt80, Als1, Mnn9, Van1, Pmr1, Gca1, and Big1) expression were upregulated in S. mutans MV-treated group. In summary, the results of the present study showed that S. mutans MVs harboring glucosyltransferases involved in exopolysaccharide production augment C. albicans biofilm development, revealing a key role for S. mutans MVs in cross-kingdom interactions between S. mutans and C. albicans. |
| first_indexed | 2024-04-13T22:13:03Z |
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| id | doaj.art-b4428cc78a0e477d8082d839d9af8e36 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-13T22:13:03Z |
| publishDate | 2020-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-b4428cc78a0e477d8082d839d9af8e362022-12-22T02:27:39ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-09-011110.3389/fmicb.2020.581184581184Streptococcus mutans Membrane Vesicles Harboring Glucosyltransferases Augment Candida albicans Biofilm DevelopmentRuixue Wu0Ruixue Wu1Ye Tao2Ye Tao3Yina Cao4Yina Cao5Yan Zhou6Yan Zhou7Huancai Lin8Huancai Lin9Department of Preventive Dentistry, Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, ChinaDepartment of Preventive Dentistry, Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, ChinaDepartment of Preventive Dentistry, Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, ChinaDepartment of Preventive Dentistry, Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, ChinaDepartment of Preventive Dentistry, Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, ChinaCandida albicans, as the most common fungus in the oral cavity, is often detected in early childhood caries. Streptococcus mutans is the major etiological agent of dental caries, but the role of S. mutans on C. albicans growth and biofilm development remains to be elucidated. Membrane vesicles (MVs) are a cell-secreted subcellular fraction that play an important role in intercellular communication and disease progression. In the present study, we investigated whether MVs from S. mutans augment C. albicans growth and biofilm development. The results indicated that S. mutans MVs augmented C. albicans biofilm development but had no significant effect on C. albicans growth under planktonic conditions. Subsequently, we labeled S. mutans MVs with PKH26 and used confocal laser scanning microscopy (CLSM) to track S. mutans MVs, which were observed to be located in the C. albicans biofilm extracellular matrix. Monosaccharide tests showed that S. mutans MVs contribute to sucrose metabolism in C. albicans. Polysaccharides were significantly enriched in the S. mutans MV-treated group. MVs from ΔgtfBC mutant strains were compared with those from the wild-type S. mutans. The results revealed that MVs from the ΔgtfBC mutant had no effect on C. albicans biofilm formation and exopolysaccharide production. In addition, C. albicans biofilm transcriptional regulators (Ndt80, Als1, Mnn9, Van1, Pmr1, Gca1, and Big1) expression were upregulated in S. mutans MV-treated group. In summary, the results of the present study showed that S. mutans MVs harboring glucosyltransferases involved in exopolysaccharide production augment C. albicans biofilm development, revealing a key role for S. mutans MVs in cross-kingdom interactions between S. mutans and C. albicans.https://www.frontiersin.org/article/10.3389/fmicb.2020.581184/fullStreptococcus mutansCandida albicansmembrane vesiclesglucosyltransferasesexopolysaccharides |
| spellingShingle | Ruixue Wu Ruixue Wu Ye Tao Ye Tao Yina Cao Yina Cao Yan Zhou Yan Zhou Huancai Lin Huancai Lin Streptococcus mutans Membrane Vesicles Harboring Glucosyltransferases Augment Candida albicans Biofilm Development Frontiers in Microbiology Streptococcus mutans Candida albicans membrane vesicles glucosyltransferases exopolysaccharides |
| title | Streptococcus mutans Membrane Vesicles Harboring Glucosyltransferases Augment Candida albicans Biofilm Development |
| title_full | Streptococcus mutans Membrane Vesicles Harboring Glucosyltransferases Augment Candida albicans Biofilm Development |
| title_fullStr | Streptococcus mutans Membrane Vesicles Harboring Glucosyltransferases Augment Candida albicans Biofilm Development |
| title_full_unstemmed | Streptococcus mutans Membrane Vesicles Harboring Glucosyltransferases Augment Candida albicans Biofilm Development |
| title_short | Streptococcus mutans Membrane Vesicles Harboring Glucosyltransferases Augment Candida albicans Biofilm Development |
| title_sort | streptococcus mutans membrane vesicles harboring glucosyltransferases augment candida albicans biofilm development |
| topic | Streptococcus mutans Candida albicans membrane vesicles glucosyltransferases exopolysaccharides |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2020.581184/full |
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