Effects of Post-Fermentation Temperature on Amino Nitrogen Formation Kinetics and Physicochemical Properties of Bacterial-type Douchi from Different Soybean Varieties

In order to improve the quality stability of bacterial-type douchi during industrial production, four different varieties of soybean (LS, DBL, DBS and BS) were used to make bacterial-type douchi, and the modified Gompertz equation was used to fit the changes in the amino nitrogen content of bacteria...

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Main Author: LI Aijun, WANG Di, YANG Gang, SONG Jun, KONG Xiangbing, LIAO Shenglan, KAN Jianquan
Format: Article
Language:English
Published: China Food Publishing Company 2023-01-01
Series:Shipin Kexue
Subjects:
Online Access:https://www.spkx.net.cn/fileup/1002-6630/PDF/2023-44-2-026.pdf
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author LI Aijun, WANG Di, YANG Gang, SONG Jun, KONG Xiangbing, LIAO Shenglan, KAN Jianquan
author_facet LI Aijun, WANG Di, YANG Gang, SONG Jun, KONG Xiangbing, LIAO Shenglan, KAN Jianquan
author_sort LI Aijun, WANG Di, YANG Gang, SONG Jun, KONG Xiangbing, LIAO Shenglan, KAN Jianquan
collection DOAJ
description In order to improve the quality stability of bacterial-type douchi during industrial production, four different varieties of soybean (LS, DBL, DBS and BS) were used to make bacterial-type douchi, and the modified Gompertz equation was used to fit the changes in the amino nitrogen content of bacterial-type douchi at three different post-fermentation temperatures (15, 25 and 40 ℃). Also, the free amino acid content was determined at the end of fermentation. The results showed that the Gompertz equation could well fit the kinetics of amino nitrogen formation (coefficient of determination > 0.96). The predicted fermentation endpoints at post-fermentation temperatures of 25, 15 and 40 ℃were approximately 9, 10, and 4 days, respectively. The maximum production of amino acid nitrogen in the four bacterial-type douchi at post-fermentation temperature of 15 ℃ decreased in the following order: DBL > LS > DBS > BS, while the maximum production of amino nitrogen at 25 and 40 ℃ decreased in the following order: LS > DBL > DBS > BS. The contents of amino nitrogen and free amino acids in black soybean douchi were significantly lower than those in yellow soybean douchi, but there was no significant difference among douchi from different yellow soybean varieties. After post-fermentation, the proportion of umami amino acids in the four bacterial-type douchi increased, and the proportion of bitter amino acids decreased. The proportion of umami amino acids in the four bacterial-type douchi at post-fermentation temperature of 40 ℃ decreased and the proportion of bitter amino acids increased compared with that at 15 ℃. This may be related to consumption of umami amino acids by the Maillard reaction at high temperatures. The correlation analysis of quality indexes shows that the trend of four bacterial-type douchi was similar. The results of this study can provide theoretical guidance for the industrial production of bacterial-type douchi.
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spelling doaj.art-b4479da3ae0448568d3f9d22b4fe64bb2023-03-06T07:13:02ZengChina Food Publishing CompanyShipin Kexue1002-66302023-01-0144219520310.7506/spkx1002-6630-20220309-116Effects of Post-Fermentation Temperature on Amino Nitrogen Formation Kinetics and Physicochemical Properties of Bacterial-type Douchi from Different Soybean VarietiesLI Aijun, WANG Di, YANG Gang, SONG Jun, KONG Xiangbing, LIAO Shenglan, KAN Jianquan0(1. Laboratory of Quality & Safety Risk Assessment for Agri-products on Storage and Preservation, Ministry of Agriculture and Rural Affairs, Chinese-Hungarian Cooperative Research Centre for Food Science, Chongqing Key Laboratory of Speciality Food Co-Built by Sichuan and Chongqing, College of Food Science, Southwest University, Chongqing 400715, China; 2. Junyi Food Factory, Chongqing 402160, China)In order to improve the quality stability of bacterial-type douchi during industrial production, four different varieties of soybean (LS, DBL, DBS and BS) were used to make bacterial-type douchi, and the modified Gompertz equation was used to fit the changes in the amino nitrogen content of bacterial-type douchi at three different post-fermentation temperatures (15, 25 and 40 ℃). Also, the free amino acid content was determined at the end of fermentation. The results showed that the Gompertz equation could well fit the kinetics of amino nitrogen formation (coefficient of determination > 0.96). The predicted fermentation endpoints at post-fermentation temperatures of 25, 15 and 40 ℃were approximately 9, 10, and 4 days, respectively. The maximum production of amino acid nitrogen in the four bacterial-type douchi at post-fermentation temperature of 15 ℃ decreased in the following order: DBL > LS > DBS > BS, while the maximum production of amino nitrogen at 25 and 40 ℃ decreased in the following order: LS > DBL > DBS > BS. The contents of amino nitrogen and free amino acids in black soybean douchi were significantly lower than those in yellow soybean douchi, but there was no significant difference among douchi from different yellow soybean varieties. After post-fermentation, the proportion of umami amino acids in the four bacterial-type douchi increased, and the proportion of bitter amino acids decreased. The proportion of umami amino acids in the four bacterial-type douchi at post-fermentation temperature of 40 ℃ decreased and the proportion of bitter amino acids increased compared with that at 15 ℃. This may be related to consumption of umami amino acids by the Maillard reaction at high temperatures. The correlation analysis of quality indexes shows that the trend of four bacterial-type douchi was similar. The results of this study can provide theoretical guidance for the industrial production of bacterial-type douchi.https://www.spkx.net.cn/fileup/1002-6630/PDF/2023-44-2-026.pdfbacterial-type douchi; soybean varieties; post-fermentation temperature; amino nitrogen; free amino acids; correlation analysis
spellingShingle LI Aijun, WANG Di, YANG Gang, SONG Jun, KONG Xiangbing, LIAO Shenglan, KAN Jianquan
Effects of Post-Fermentation Temperature on Amino Nitrogen Formation Kinetics and Physicochemical Properties of Bacterial-type Douchi from Different Soybean Varieties
Shipin Kexue
bacterial-type douchi; soybean varieties; post-fermentation temperature; amino nitrogen; free amino acids; correlation analysis
title Effects of Post-Fermentation Temperature on Amino Nitrogen Formation Kinetics and Physicochemical Properties of Bacterial-type Douchi from Different Soybean Varieties
title_full Effects of Post-Fermentation Temperature on Amino Nitrogen Formation Kinetics and Physicochemical Properties of Bacterial-type Douchi from Different Soybean Varieties
title_fullStr Effects of Post-Fermentation Temperature on Amino Nitrogen Formation Kinetics and Physicochemical Properties of Bacterial-type Douchi from Different Soybean Varieties
title_full_unstemmed Effects of Post-Fermentation Temperature on Amino Nitrogen Formation Kinetics and Physicochemical Properties of Bacterial-type Douchi from Different Soybean Varieties
title_short Effects of Post-Fermentation Temperature on Amino Nitrogen Formation Kinetics and Physicochemical Properties of Bacterial-type Douchi from Different Soybean Varieties
title_sort effects of post fermentation temperature on amino nitrogen formation kinetics and physicochemical properties of bacterial type douchi from different soybean varieties
topic bacterial-type douchi; soybean varieties; post-fermentation temperature; amino nitrogen; free amino acids; correlation analysis
url https://www.spkx.net.cn/fileup/1002-6630/PDF/2023-44-2-026.pdf
work_keys_str_mv AT liaijunwangdiyanggangsongjunkongxiangbingliaoshenglankanjianquan effectsofpostfermentationtemperatureonaminonitrogenformationkineticsandphysicochemicalpropertiesofbacterialtypedouchifromdifferentsoybeanvarieties