Human umbilical cord mesenchymal stem cells derived extracellular vesicles alleviate salpingitis by promoting M1–to–M2 transformation
Background: With an increasing number of patients experiencing infertility due to chronic salpingitis after Chlamydia trachomatis (CT) infection, there is an unmet need for tissue repair or regeneration therapies. Treatment with human umbilical cord mesenchymal stem cell-derived extracellular vesicl...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-02-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphys.2023.1131701/full |
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| author | Changlin Zhang Wei Liao Weizhao Li Mengxiong Li Xiaoyu Xu Haohui Sun Yaohua Xue Lixiang Liu Jiehong Qiu Chi Zhang Xunzhi Zhang Juntong Ye Jingran Du David Y. B. Deng Wuguo Deng Tian Li |
| author_facet | Changlin Zhang Wei Liao Weizhao Li Mengxiong Li Xiaoyu Xu Haohui Sun Yaohua Xue Lixiang Liu Jiehong Qiu Chi Zhang Xunzhi Zhang Juntong Ye Jingran Du David Y. B. Deng Wuguo Deng Tian Li |
| author_sort | Changlin Zhang |
| collection | DOAJ |
| description | Background: With an increasing number of patients experiencing infertility due to chronic salpingitis after Chlamydia trachomatis (CT) infection, there is an unmet need for tissue repair or regeneration therapies. Treatment with human umbilical cord mesenchymal stem cell-derived extracellular vesicles (hucMSC-EV) provides an attractive cell-free therapeutic approach.Methods: In this study, we investigated the alleviating effect of hucMSC-EV on tubal inflammatory infertility caused by CT using in vivo animal experiments. Furthermore, we examined the effect of hucMSC-EV on inducing macrophage polarization to explore the molecular mechanism.Results: Our results showed that tubal inflammatory infertility caused by Chlamydia infection was significantly alleviated in the hucMSC-EV treatment group compared with the control group. Further mechanistic experiments showed that the application of hucMSC-EV induced macrophage polarization from the M1 to the M2 type via the NF-κB signaling pathway, improved the local inflammatory microenvironment of fallopian tubes and inhibited tube inflammation.Conclusion: We conclude that this approach represents a promising cell-free avenue to ameliorate infertility due to chronic salpingitis. |
| first_indexed | 2024-04-10T10:03:53Z |
| format | Article |
| id | doaj.art-b44836939ff74bba9d5e3f98b32a7362 |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-04-10T10:03:53Z |
| publishDate | 2023-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-b44836939ff74bba9d5e3f98b32a73622023-02-16T04:52:51ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2023-02-011410.3389/fphys.2023.11317011131701Human umbilical cord mesenchymal stem cells derived extracellular vesicles alleviate salpingitis by promoting M1–to–M2 transformationChanglin Zhang0Wei Liao1Weizhao Li2Mengxiong Li3Xiaoyu Xu4Haohui Sun5Yaohua Xue6Lixiang Liu7Jiehong Qiu8Chi Zhang9Xunzhi Zhang10Juntong Ye11Jingran Du12David Y. B. Deng13Wuguo Deng14Tian Li15Pelvic Floor Disorders Center, Scientific Research Center, Department of Gynecology, The Seventh Affiliated Hospital of Sun Yat-sen University, Shenzhen, ChinaPelvic Floor Disorders Center, Scientific Research Center, Department of Gynecology, The Seventh Affiliated Hospital of Sun Yat-sen University, Shenzhen, ChinaPelvic Floor Disorders Center, Scientific Research Center, Department of Gynecology, The Seventh Affiliated Hospital of Sun Yat-sen University, Shenzhen, ChinaPelvic Floor Disorders Center, Scientific Research Center, Department of Gynecology, The Seventh Affiliated Hospital of Sun Yat-sen University, Shenzhen, ChinaPelvic Floor Disorders Center, Scientific Research Center, Department of Gynecology, The Seventh Affiliated Hospital of Sun Yat-sen University, Shenzhen, ChinaSun Yat-Sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center of Cancer Medicine, Guangzhou, ChinaDepartment of Clinical Laboratory, Dermatology Hospital, Southern Medical University, Guangzhou, ChinaPelvic Floor Disorders Center, Scientific Research Center, Department of Gynecology, The Seventh Affiliated Hospital of Sun Yat-sen University, Shenzhen, ChinaPelvic Floor Disorders Center, Scientific Research Center, Department of Gynecology, The Seventh Affiliated Hospital of Sun Yat-sen University, Shenzhen, ChinaPelvic Floor Disorders Center, Scientific Research Center, Department of Gynecology, The Seventh Affiliated Hospital of Sun Yat-sen University, Shenzhen, ChinaCollege of Life Sciences and Oceanography, Shenzhen University, Shenzhen, ChinaPelvic Floor Disorders Center, Scientific Research Center, Department of Gynecology, The Seventh Affiliated Hospital of Sun Yat-sen University, Shenzhen, ChinaPelvic Floor Disorders Center, Scientific Research Center, Department of Gynecology, The Seventh Affiliated Hospital of Sun Yat-sen University, Shenzhen, ChinaPelvic Floor Disorders Center, Scientific Research Center, Department of Gynecology, The Seventh Affiliated Hospital of Sun Yat-sen University, Shenzhen, ChinaSun Yat-Sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center of Cancer Medicine, Guangzhou, ChinaPelvic Floor Disorders Center, Scientific Research Center, Department of Gynecology, The Seventh Affiliated Hospital of Sun Yat-sen University, Shenzhen, ChinaBackground: With an increasing number of patients experiencing infertility due to chronic salpingitis after Chlamydia trachomatis (CT) infection, there is an unmet need for tissue repair or regeneration therapies. Treatment with human umbilical cord mesenchymal stem cell-derived extracellular vesicles (hucMSC-EV) provides an attractive cell-free therapeutic approach.Methods: In this study, we investigated the alleviating effect of hucMSC-EV on tubal inflammatory infertility caused by CT using in vivo animal experiments. Furthermore, we examined the effect of hucMSC-EV on inducing macrophage polarization to explore the molecular mechanism.Results: Our results showed that tubal inflammatory infertility caused by Chlamydia infection was significantly alleviated in the hucMSC-EV treatment group compared with the control group. Further mechanistic experiments showed that the application of hucMSC-EV induced macrophage polarization from the M1 to the M2 type via the NF-κB signaling pathway, improved the local inflammatory microenvironment of fallopian tubes and inhibited tube inflammation.Conclusion: We conclude that this approach represents a promising cell-free avenue to ameliorate infertility due to chronic salpingitis.https://www.frontiersin.org/articles/10.3389/fphys.2023.1131701/fullchronic salpingitismacrophage polarizationNF-κB signaling pathwaymesenchymal stem cell (MSC)extracellular vesicles |
| spellingShingle | Changlin Zhang Wei Liao Weizhao Li Mengxiong Li Xiaoyu Xu Haohui Sun Yaohua Xue Lixiang Liu Jiehong Qiu Chi Zhang Xunzhi Zhang Juntong Ye Jingran Du David Y. B. Deng Wuguo Deng Tian Li Human umbilical cord mesenchymal stem cells derived extracellular vesicles alleviate salpingitis by promoting M1–to–M2 transformation Frontiers in Physiology chronic salpingitis macrophage polarization NF-κB signaling pathway mesenchymal stem cell (MSC) extracellular vesicles |
| title | Human umbilical cord mesenchymal stem cells derived extracellular vesicles alleviate salpingitis by promoting M1–to–M2 transformation |
| title_full | Human umbilical cord mesenchymal stem cells derived extracellular vesicles alleviate salpingitis by promoting M1–to–M2 transformation |
| title_fullStr | Human umbilical cord mesenchymal stem cells derived extracellular vesicles alleviate salpingitis by promoting M1–to–M2 transformation |
| title_full_unstemmed | Human umbilical cord mesenchymal stem cells derived extracellular vesicles alleviate salpingitis by promoting M1–to–M2 transformation |
| title_short | Human umbilical cord mesenchymal stem cells derived extracellular vesicles alleviate salpingitis by promoting M1–to–M2 transformation |
| title_sort | human umbilical cord mesenchymal stem cells derived extracellular vesicles alleviate salpingitis by promoting m1 to m2 transformation |
| topic | chronic salpingitis macrophage polarization NF-κB signaling pathway mesenchymal stem cell (MSC) extracellular vesicles |
| url | https://www.frontiersin.org/articles/10.3389/fphys.2023.1131701/full |
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