Expression of delta-catenin is associated with progression of human astrocytoma
<p>Abstract</p> <p>Background</p> <p>δ-Catenin (<it>CTNND2</it>), which encodes a scaffold protein in humans, has been found in a few malignancies. However, the expression pattern and contribution of δ-catenin to astrocytoma progression are unclear.</p>...
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| Format: | Article |
| Language: | English |
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BMC
2011-12-01
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| Series: | BMC Cancer |
| Online Access: | http://www.biomedcentral.com/1471-2407/11/514 |
| _version_ | 1828739133918412800 |
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| author | MingHao Wang Qianze Dong Di Zhang YunJie Wang |
| author_facet | MingHao Wang Qianze Dong Di Zhang YunJie Wang |
| author_sort | MingHao Wang |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>δ-Catenin (<it>CTNND2</it>), which encodes a scaffold protein in humans, has been found in a few malignancies. However, the expression pattern and contribution of δ-catenin to astrocytoma progression are unclear.</p> <p>Methods</p> <p>We investigated δ-catenin expression in human astrocytoma samples and its function in astrocytoma cell lines using immunohistochemistry, siRNA knockdown, transfection, MTT, transwell migration and Rac1 pulldown techniques.</p> <p>Results</p> <p>δ-Catenin protein expression was detected in cytoplasm of astrocytoma cells by immunohistochemistry. Analysis showed that grade I astrocytoma (0%, 0/11) and glial cells from normal brain tissue exhibited negative staining. δ-Catenin expression was significantly higher in grade III-IV (35%, 29/84) compared to grade II astrocytoma cells (18%, 11/61); <it>p </it>< 0.01). In addition, <it>CTNND2 </it>overexpression promoted proliferation, invasion and Rac1 activity of U251 astrocytoma cells. Treatment of δ-catenin-transfected cells with a Rac1 inhibitor decreased Rac1 activity and invasion. δ-Catenin knockdown in U87 glioblastoma cell decreased cell proliferation, invasion and Rac1 activity.</p> <p>Conclusion</p> <p>The results suggest that δ-catenin expression is associated with the malignant progression of astrocytoma and promotes astrocytoma cell invasion through upregulation of Rac1 activity. δ-Catenin expression levels may serve as a useful marker of the biological behavior of astrocytoma cells.</p> |
| first_indexed | 2024-04-13T00:17:08Z |
| format | Article |
| id | doaj.art-b4487dab9c5448caa2d9622fed4caed9 |
| institution | Directory Open Access Journal |
| issn | 1471-2407 |
| language | English |
| last_indexed | 2024-04-13T00:17:08Z |
| publishDate | 2011-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj.art-b4487dab9c5448caa2d9622fed4caed92022-12-22T03:10:55ZengBMCBMC Cancer1471-24072011-12-0111151410.1186/1471-2407-11-514Expression of delta-catenin is associated with progression of human astrocytomaMingHao WangQianze DongDi ZhangYunJie Wang<p>Abstract</p> <p>Background</p> <p>δ-Catenin (<it>CTNND2</it>), which encodes a scaffold protein in humans, has been found in a few malignancies. However, the expression pattern and contribution of δ-catenin to astrocytoma progression are unclear.</p> <p>Methods</p> <p>We investigated δ-catenin expression in human astrocytoma samples and its function in astrocytoma cell lines using immunohistochemistry, siRNA knockdown, transfection, MTT, transwell migration and Rac1 pulldown techniques.</p> <p>Results</p> <p>δ-Catenin protein expression was detected in cytoplasm of astrocytoma cells by immunohistochemistry. Analysis showed that grade I astrocytoma (0%, 0/11) and glial cells from normal brain tissue exhibited negative staining. δ-Catenin expression was significantly higher in grade III-IV (35%, 29/84) compared to grade II astrocytoma cells (18%, 11/61); <it>p </it>< 0.01). In addition, <it>CTNND2 </it>overexpression promoted proliferation, invasion and Rac1 activity of U251 astrocytoma cells. Treatment of δ-catenin-transfected cells with a Rac1 inhibitor decreased Rac1 activity and invasion. δ-Catenin knockdown in U87 glioblastoma cell decreased cell proliferation, invasion and Rac1 activity.</p> <p>Conclusion</p> <p>The results suggest that δ-catenin expression is associated with the malignant progression of astrocytoma and promotes astrocytoma cell invasion through upregulation of Rac1 activity. δ-Catenin expression levels may serve as a useful marker of the biological behavior of astrocytoma cells.</p>http://www.biomedcentral.com/1471-2407/11/514 |
| spellingShingle | MingHao Wang Qianze Dong Di Zhang YunJie Wang Expression of delta-catenin is associated with progression of human astrocytoma BMC Cancer |
| title | Expression of delta-catenin is associated with progression of human astrocytoma |
| title_full | Expression of delta-catenin is associated with progression of human astrocytoma |
| title_fullStr | Expression of delta-catenin is associated with progression of human astrocytoma |
| title_full_unstemmed | Expression of delta-catenin is associated with progression of human astrocytoma |
| title_short | Expression of delta-catenin is associated with progression of human astrocytoma |
| title_sort | expression of delta catenin is associated with progression of human astrocytoma |
| url | http://www.biomedcentral.com/1471-2407/11/514 |
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