Genetic Basis of Maize Resistance to Multiple Insect Pests: Integrated Genome-Wide Comparative Mapping and Candidate Gene Prioritization
Several species of herbivores feed on maize in field and storage setups, making the development of multiple insect resistance a critical breeding target. In this study, an association mapping panel of 341 tropical maize lines was evaluated in three field environments for resistance to fall armyworm...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-06-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/11/6/689 |
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| author | A. Badji D. B. Kwemoi L. Machida D. Okii N. Mwila S. Agbahoungba F. Kumi A. Ibanda A. Bararyenya M. Solemanegy T. Odong P. Wasswa M. Otim G. Asea M. Ochwo-Ssemakula H. Talwana S. Kyamanywa P. Rubaihayo |
| author_facet | A. Badji D. B. Kwemoi L. Machida D. Okii N. Mwila S. Agbahoungba F. Kumi A. Ibanda A. Bararyenya M. Solemanegy T. Odong P. Wasswa M. Otim G. Asea M. Ochwo-Ssemakula H. Talwana S. Kyamanywa P. Rubaihayo |
| author_sort | A. Badji |
| collection | DOAJ |
| description | Several species of herbivores feed on maize in field and storage setups, making the development of multiple insect resistance a critical breeding target. In this study, an association mapping panel of 341 tropical maize lines was evaluated in three field environments for resistance to fall armyworm (FAW), whilst bulked grains were subjected to a maize weevil (MW) bioassay and genotyped with Diversity Array Technology’s single nucleotide polymorphisms (SNPs) markers. A multi-locus genome-wide association study (GWAS) revealed 62 quantitative trait nucleotides (QTNs) associated with FAW and MW resistance traits on all 10 maize chromosomes, of which, 47 and 31 were discovered at stringent Bonferroni genome-wide significance levels of 0.05 and 0.01, respectively, and located within or close to multiple insect resistance genomic regions (MIRGRs) concerning FAW, SB, and MW. Sixteen QTNs influenced multiple traits, of which, six were associated with resistance to both FAW and MW, suggesting a pleiotropic genetic control. Functional prioritization of candidate genes (CGs) located within 10–30 kb of the QTNs revealed 64 putative GWAS-based CGs (GbCGs) showing evidence of involvement in plant defense mechanisms. Only one GbCG was associated with each of the five of the six combined resistance QTNs, thus reinforcing the pleiotropy hypothesis. In addition, through in silico co-functional network inferences, an additional 107 network-based CGs (NbCGs), biologically connected to the 64 GbCGs, and differentially expressed under biotic or abiotic stress, were revealed within MIRGRs. The provided multiple insect resistance physical map should contribute to the development of combined insect resistance in maize. |
| first_indexed | 2024-03-10T18:55:35Z |
| format | Article |
| id | doaj.art-b449302a503a42ef80413fb5d6087a43 |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-10T18:55:35Z |
| publishDate | 2020-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-b449302a503a42ef80413fb5d6087a432023-11-20T04:47:05ZengMDPI AGGenes2073-44252020-06-0111668910.3390/genes11060689Genetic Basis of Maize Resistance to Multiple Insect Pests: Integrated Genome-Wide Comparative Mapping and Candidate Gene PrioritizationA. Badji0D. B. Kwemoi1L. Machida2D. Okii3N. Mwila4S. Agbahoungba5F. Kumi6A. Ibanda7A. Bararyenya8M. Solemanegy9T. Odong10P. Wasswa11M. Otim12G. Asea13M. Ochwo-Ssemakula14H. Talwana15S. Kyamanywa16P. Rubaihayo17Department of Agricultural Production, Makerere Univesity, P.O. Box 7062 Kampala, UgandaCereals Program, National Crop Resource Research Institute, P.O. Box 7084 Kampala, UgandaAlliance Bioversity International-CIAT, P.O. Box 24384 Kampala, UgandaDepartment of Agricultural Production, Makerere Univesity, P.O. Box 7062 Kampala, UgandaDepartment of Agricultural Production, Makerere Univesity, P.O. Box 7062 Kampala, UgandaLaboratory of Applied Ecology, University of Abomey-Calavi, 01BP 526 Cotonou, BeninDepartment of Crop Science, University of Cape Coast, P.O. Box 5007 PMB Cape Coast, GhanaDepartment of Agricultural Production, Makerere Univesity, P.O. Box 7062 Kampala, UgandaDepartment of Agricultural Production, Makerere Univesity, P.O. Box 7062 Kampala, UgandaDepartment of Agricultural Production, Makerere Univesity, P.O. Box 7062 Kampala, UgandaDepartment of Agricultural Production, Makerere Univesity, P.O. Box 7062 Kampala, UgandaDepartment of Agricultural Production, Makerere Univesity, P.O. Box 7062 Kampala, UgandaCereals Program, National Crop Resource Research Institute, P.O. Box 7084 Kampala, UgandaCereals Program, National Crop Resource Research Institute, P.O. Box 7084 Kampala, UgandaDepartment of Agricultural Production, Makerere Univesity, P.O. Box 7062 Kampala, UgandaDepartment of Agricultural Production, Makerere Univesity, P.O. Box 7062 Kampala, UgandaDepartment of Agricultural Production, Makerere Univesity, P.O. Box 7062 Kampala, UgandaDepartment of Agricultural Production, Makerere Univesity, P.O. Box 7062 Kampala, UgandaSeveral species of herbivores feed on maize in field and storage setups, making the development of multiple insect resistance a critical breeding target. In this study, an association mapping panel of 341 tropical maize lines was evaluated in three field environments for resistance to fall armyworm (FAW), whilst bulked grains were subjected to a maize weevil (MW) bioassay and genotyped with Diversity Array Technology’s single nucleotide polymorphisms (SNPs) markers. A multi-locus genome-wide association study (GWAS) revealed 62 quantitative trait nucleotides (QTNs) associated with FAW and MW resistance traits on all 10 maize chromosomes, of which, 47 and 31 were discovered at stringent Bonferroni genome-wide significance levels of 0.05 and 0.01, respectively, and located within or close to multiple insect resistance genomic regions (MIRGRs) concerning FAW, SB, and MW. Sixteen QTNs influenced multiple traits, of which, six were associated with resistance to both FAW and MW, suggesting a pleiotropic genetic control. Functional prioritization of candidate genes (CGs) located within 10–30 kb of the QTNs revealed 64 putative GWAS-based CGs (GbCGs) showing evidence of involvement in plant defense mechanisms. Only one GbCG was associated with each of the five of the six combined resistance QTNs, thus reinforcing the pleiotropy hypothesis. In addition, through in silico co-functional network inferences, an additional 107 network-based CGs (NbCGs), biologically connected to the 64 GbCGs, and differentially expressed under biotic or abiotic stress, were revealed within MIRGRs. The provided multiple insect resistance physical map should contribute to the development of combined insect resistance in maize.https://www.mdpi.com/2073-4425/11/6/689combined insect resistanceQTNsfunctional prioritizationfall armywormmaize weevilstem borers |
| spellingShingle | A. Badji D. B. Kwemoi L. Machida D. Okii N. Mwila S. Agbahoungba F. Kumi A. Ibanda A. Bararyenya M. Solemanegy T. Odong P. Wasswa M. Otim G. Asea M. Ochwo-Ssemakula H. Talwana S. Kyamanywa P. Rubaihayo Genetic Basis of Maize Resistance to Multiple Insect Pests: Integrated Genome-Wide Comparative Mapping and Candidate Gene Prioritization Genes combined insect resistance QTNs functional prioritization fall armyworm maize weevil stem borers |
| title | Genetic Basis of Maize Resistance to Multiple Insect Pests: Integrated Genome-Wide Comparative Mapping and Candidate Gene Prioritization |
| title_full | Genetic Basis of Maize Resistance to Multiple Insect Pests: Integrated Genome-Wide Comparative Mapping and Candidate Gene Prioritization |
| title_fullStr | Genetic Basis of Maize Resistance to Multiple Insect Pests: Integrated Genome-Wide Comparative Mapping and Candidate Gene Prioritization |
| title_full_unstemmed | Genetic Basis of Maize Resistance to Multiple Insect Pests: Integrated Genome-Wide Comparative Mapping and Candidate Gene Prioritization |
| title_short | Genetic Basis of Maize Resistance to Multiple Insect Pests: Integrated Genome-Wide Comparative Mapping and Candidate Gene Prioritization |
| title_sort | genetic basis of maize resistance to multiple insect pests integrated genome wide comparative mapping and candidate gene prioritization |
| topic | combined insect resistance QTNs functional prioritization fall armyworm maize weevil stem borers |
| url | https://www.mdpi.com/2073-4425/11/6/689 |
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