Sample Delivery Systems for Serial Femtosecond Crystallography at the PAL-XFEL
Serial femtosecond crystallography (SFX) using an X-ray free electron laser (XFEL) enables the determination of room-temperature structures without causing radiation damage. Using an optical pump-probe or mix-and-injection, SFX enables the intermediate state visualization of a molecular reaction. In...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-05-01
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| Series: | Photonics |
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| Online Access: | https://www.mdpi.com/2304-6732/10/5/557 |
| _version_ | 1797598619833991168 |
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| author | Jaehyun Park Ki Hyun Nam |
| author_facet | Jaehyun Park Ki Hyun Nam |
| author_sort | Jaehyun Park |
| collection | DOAJ |
| description | Serial femtosecond crystallography (SFX) using an X-ray free electron laser (XFEL) enables the determination of room-temperature structures without causing radiation damage. Using an optical pump-probe or mix-and-injection, SFX enables the intermediate state visualization of a molecular reaction. In SFX experiments, serial and stable microcrystal delivery to the X-ray interaction point is vital for reasonable data collection and efficient beam time. The Pohang Accelerator Laboratory X-ray Free Electron Laser (PAL-XFEL) facility established SFX instruments at a nanocrystallography and coherent imaging (NCI) experimental station. Various sample delivery methods, including injection, fixed-target scanning, and hybrid methods, have been developed and applied to collect XFEL diffraction data. Herein, we report the currently available sample delivery methods for SFX at the NCI experimental station at the PAL-XFEL. This article will help PAL-XFEL users access the SFX system for their experiments. |
| first_indexed | 2024-03-11T03:23:41Z |
| format | Article |
| id | doaj.art-b449cd40f7914776972d18970a522c39 |
| institution | Directory Open Access Journal |
| issn | 2304-6732 |
| language | English |
| last_indexed | 2024-03-11T03:23:41Z |
| publishDate | 2023-05-01 |
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| record_format | Article |
| series | Photonics |
| spelling | doaj.art-b449cd40f7914776972d18970a522c392023-11-18T02:54:26ZengMDPI AGPhotonics2304-67322023-05-0110555710.3390/photonics10050557Sample Delivery Systems for Serial Femtosecond Crystallography at the PAL-XFELJaehyun Park0Ki Hyun Nam1Pohang Accelerator Laboratory, Pohang University of Science and Technology, Pohang 37673, Republic of KoreaDepartment of Life Science, Pohang University of Science and Technology, Pohang 37673, Republic of KoreaSerial femtosecond crystallography (SFX) using an X-ray free electron laser (XFEL) enables the determination of room-temperature structures without causing radiation damage. Using an optical pump-probe or mix-and-injection, SFX enables the intermediate state visualization of a molecular reaction. In SFX experiments, serial and stable microcrystal delivery to the X-ray interaction point is vital for reasonable data collection and efficient beam time. The Pohang Accelerator Laboratory X-ray Free Electron Laser (PAL-XFEL) facility established SFX instruments at a nanocrystallography and coherent imaging (NCI) experimental station. Various sample delivery methods, including injection, fixed-target scanning, and hybrid methods, have been developed and applied to collect XFEL diffraction data. Herein, we report the currently available sample delivery methods for SFX at the NCI experimental station at the PAL-XFEL. This article will help PAL-XFEL users access the SFX system for their experiments.https://www.mdpi.com/2304-6732/10/5/557serial femtosecond crystallographyX-ray free electron lasersample deliveryinjectorfixed-target scanninghybrid method |
| spellingShingle | Jaehyun Park Ki Hyun Nam Sample Delivery Systems for Serial Femtosecond Crystallography at the PAL-XFEL Photonics serial femtosecond crystallography X-ray free electron laser sample delivery injector fixed-target scanning hybrid method |
| title | Sample Delivery Systems for Serial Femtosecond Crystallography at the PAL-XFEL |
| title_full | Sample Delivery Systems for Serial Femtosecond Crystallography at the PAL-XFEL |
| title_fullStr | Sample Delivery Systems for Serial Femtosecond Crystallography at the PAL-XFEL |
| title_full_unstemmed | Sample Delivery Systems for Serial Femtosecond Crystallography at the PAL-XFEL |
| title_short | Sample Delivery Systems for Serial Femtosecond Crystallography at the PAL-XFEL |
| title_sort | sample delivery systems for serial femtosecond crystallography at the pal xfel |
| topic | serial femtosecond crystallography X-ray free electron laser sample delivery injector fixed-target scanning hybrid method |
| url | https://www.mdpi.com/2304-6732/10/5/557 |
| work_keys_str_mv | AT jaehyunpark sampledeliverysystemsforserialfemtosecondcrystallographyatthepalxfel AT kihyunnam sampledeliverysystemsforserialfemtosecondcrystallographyatthepalxfel |