Digging deeper into lymphatic vessel formation <it>in vitro </it>and <it>in vivo</it>
<p>Abstract</p> <p>Background</p> <p>Abnormal lymphatic vessel formation (lymphangiogenesis) is associated with different pathologies such as cancer, lymphedema, psoriasis and graft rejection. Lymphatic vasculature displays distinctive features than blood vasculature, a...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2011-06-01
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| Series: | BMC Cell Biology |
| Online Access: | http://www.biomedcentral.com/1471-2121/12/29 |
| _version_ | 1811311870258184192 |
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| author | Thiry Marc Foidart Jean-Michel Lenoir Bénédicte Maillard Catherine Lamaye Françoise Paupert Jenny Erpicum Charlotte Bruyère Françoise Detry Benoit Noël Agnès |
| author_facet | Thiry Marc Foidart Jean-Michel Lenoir Bénédicte Maillard Catherine Lamaye Françoise Paupert Jenny Erpicum Charlotte Bruyère Françoise Detry Benoit Noël Agnès |
| author_sort | Thiry Marc |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Abnormal lymphatic vessel formation (lymphangiogenesis) is associated with different pathologies such as cancer, lymphedema, psoriasis and graft rejection. Lymphatic vasculature displays distinctive features than blood vasculature, and mechanisms underlying the formation of new lymphatic vessels during physiological and pathological processes are still poorly documented. Most studies on lymphatic vessel formation are focused on organism development rather than lymphangiogenic events occurring in adults. We have here studied lymphatic vessel formation in two <it>in vivo </it>models of pathological lymphangiogenesis (corneal assay and lymphangioma). These data have been confronted to those generated in the recently set up <it>in vitro </it>model of lymphatic ring assay. Ultrastructural analyses through Transmission Electron Microscopy (TEM) were performed to investigate tube morphogenesis, an important differentiating process observed during endothelial cell organization into capillary structures.</p> <p>Results</p> <p>In both <it>in vivo </it>models (lymphangiogenic corneal assay and lymphangioma), migrating lymphatic endothelial cells extended long processes exploring the neighboring environment and organized into cord-like structures. Signs of intense extracellular matrix remodeling were observed extracellularly and inside cytoplasmic vacuoles. The formation of intercellular spaces between endothelial cells led to tube formation. Proliferating lymphatic endothelial cells were detected both at the tips of sprouting capillaries and inside extending sprouts. The different steps of lymphangiogenesis observed <it>in vivo </it>are fully recapitulated <it>in vitro</it>, in the lymphatic ring assay and include: (1) endothelial cell alignment in cord like structure, (2) intracellular vacuole formation and (3) matrix degradation.</p> <p>Conclusions</p> <p>In this study, we are providing evidence for lymphatic vessel formation through tunneling relying on extensive matrix remodeling, migration and alignment of sprouting endothelial cells into tubular structures. In addition, our data emphasize the suitability of the lymphatic ring assay to unravel mechanisms underlying lymphangiogenesis.</p> |
| first_indexed | 2024-04-13T10:26:30Z |
| format | Article |
| id | doaj.art-b449cdc35a9c48519ebdf4b64c547cb6 |
| institution | Directory Open Access Journal |
| issn | 1471-2121 |
| language | English |
| last_indexed | 2024-04-13T10:26:30Z |
| publishDate | 2011-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cell Biology |
| spelling | doaj.art-b449cdc35a9c48519ebdf4b64c547cb62022-12-22T02:50:18ZengBMCBMC Cell Biology1471-21212011-06-011212910.1186/1471-2121-12-29Digging deeper into lymphatic vessel formation <it>in vitro </it>and <it>in vivo</it>Thiry MarcFoidart Jean-MichelLenoir BénédicteMaillard CatherineLamaye FrançoisePaupert JennyErpicum CharlotteBruyère FrançoiseDetry BenoitNoël Agnès<p>Abstract</p> <p>Background</p> <p>Abnormal lymphatic vessel formation (lymphangiogenesis) is associated with different pathologies such as cancer, lymphedema, psoriasis and graft rejection. Lymphatic vasculature displays distinctive features than blood vasculature, and mechanisms underlying the formation of new lymphatic vessels during physiological and pathological processes are still poorly documented. Most studies on lymphatic vessel formation are focused on organism development rather than lymphangiogenic events occurring in adults. We have here studied lymphatic vessel formation in two <it>in vivo </it>models of pathological lymphangiogenesis (corneal assay and lymphangioma). These data have been confronted to those generated in the recently set up <it>in vitro </it>model of lymphatic ring assay. Ultrastructural analyses through Transmission Electron Microscopy (TEM) were performed to investigate tube morphogenesis, an important differentiating process observed during endothelial cell organization into capillary structures.</p> <p>Results</p> <p>In both <it>in vivo </it>models (lymphangiogenic corneal assay and lymphangioma), migrating lymphatic endothelial cells extended long processes exploring the neighboring environment and organized into cord-like structures. Signs of intense extracellular matrix remodeling were observed extracellularly and inside cytoplasmic vacuoles. The formation of intercellular spaces between endothelial cells led to tube formation. Proliferating lymphatic endothelial cells were detected both at the tips of sprouting capillaries and inside extending sprouts. The different steps of lymphangiogenesis observed <it>in vivo </it>are fully recapitulated <it>in vitro</it>, in the lymphatic ring assay and include: (1) endothelial cell alignment in cord like structure, (2) intracellular vacuole formation and (3) matrix degradation.</p> <p>Conclusions</p> <p>In this study, we are providing evidence for lymphatic vessel formation through tunneling relying on extensive matrix remodeling, migration and alignment of sprouting endothelial cells into tubular structures. In addition, our data emphasize the suitability of the lymphatic ring assay to unravel mechanisms underlying lymphangiogenesis.</p>http://www.biomedcentral.com/1471-2121/12/29 |
| spellingShingle | Thiry Marc Foidart Jean-Michel Lenoir Bénédicte Maillard Catherine Lamaye Françoise Paupert Jenny Erpicum Charlotte Bruyère Françoise Detry Benoit Noël Agnès Digging deeper into lymphatic vessel formation <it>in vitro </it>and <it>in vivo</it> BMC Cell Biology |
| title | Digging deeper into lymphatic vessel formation <it>in vitro </it>and <it>in vivo</it> |
| title_full | Digging deeper into lymphatic vessel formation <it>in vitro </it>and <it>in vivo</it> |
| title_fullStr | Digging deeper into lymphatic vessel formation <it>in vitro </it>and <it>in vivo</it> |
| title_full_unstemmed | Digging deeper into lymphatic vessel formation <it>in vitro </it>and <it>in vivo</it> |
| title_short | Digging deeper into lymphatic vessel formation <it>in vitro </it>and <it>in vivo</it> |
| title_sort | digging deeper into lymphatic vessel formation it in vitro it and it in vivo it |
| url | http://www.biomedcentral.com/1471-2121/12/29 |
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