Physiological Basis of Combined Stress Tolerance to Low Phosphorus and Drought in a Diverse Set of Mungbean Germplasm
To understand the physiological basis of tolerance to combined stresses to low phosphorus (P) and drought in mungbean (<i>Vigna</i><i>radiata</i> (L.) R. Wilczek), a diverse set of 100 accessions were evaluated in hydroponics at sufficient (250 μM) and low (3 μM) P and expose...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-01-01
|
| Series: | Agronomy |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4395/11/1/99 |
| _version_ | 1797415716273520640 |
|---|---|
| author | Surendra Kumar Meena Renu Pandey Sandeep Sharma Gayacharan Tarun Kumar Madan Pal Singh Harsh Kumar Dikshit |
| author_facet | Surendra Kumar Meena Renu Pandey Sandeep Sharma Gayacharan Tarun Kumar Madan Pal Singh Harsh Kumar Dikshit |
| author_sort | Surendra Kumar Meena |
| collection | DOAJ |
| description | To understand the physiological basis of tolerance to combined stresses to low phosphorus (P) and drought in mungbean (<i>Vigna</i><i>radiata</i> (L.) R. Wilczek), a diverse set of 100 accessions were evaluated in hydroponics at sufficient (250 μM) and low (3 μM) P and exposed to drought (dehydration) stress. The principal component analysis and ranking of accessions based on relative values revealed that IC280489, EC397142, IC76415, IC333090, IC507340, and IC121316 performed superior while IC119005, IC73401, IC488526, and IC325853 performed poorly in all treatments. Selected accessions were evaluated in soil under control (sufficient P, irrigated), low P (without P, irrigated), drought (sufficient P, withholding irrigation), and combined stress (low P, withholding irrigation). Under combined stress, a significant reduction in gas exchange traits (photosynthesis, stomatal conductance, transpiration, instantaneous water use efficiency) and P uptake in seed and shoot was observed under combined stress as compared to individual stresses. Among accessions, IC488526 was most sensitive while IC333090 and IC507340 exhibited tolerance to individual or combined stress. The water balance and low P adaptation traits like membrane stability index, relative water content, specific leaf weight, organic acid exudation, biomass, grain yield, and P uptake can be used as physiological markers to evaluate for agronomic performance. Accessions with considerable tolerance to low P and drought stress can be either used as ‘donors’ in Vigna breeding program or cultivated in areas with limited P and water availability or both. |
| first_indexed | 2024-03-09T05:52:32Z |
| format | Article |
| id | doaj.art-b451dae2d75844b3805ed748c5edb63c |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-09T05:52:32Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-b451dae2d75844b3805ed748c5edb63c2023-12-03T12:16:27ZengMDPI AGAgronomy2073-43952021-01-011119910.3390/agronomy11010099Physiological Basis of Combined Stress Tolerance to Low Phosphorus and Drought in a Diverse Set of Mungbean GermplasmSurendra Kumar Meena0Renu Pandey1Sandeep Sharma2Gayacharan3Tarun Kumar4Madan Pal Singh5Harsh Kumar Dikshit6Division of Plant Physiology, ICAR-Indian Agricultural Research Institute, New Delhi 110012, IndiaDivision of Plant Physiology, ICAR-Indian Agricultural Research Institute, New Delhi 110012, IndiaDivision of Plant Physiology, ICAR-Indian Agricultural Research Institute, New Delhi 110012, IndiaDivision of Germplasm Evaluation, ICAR-National Bureau of Plant Genetic Resources, New Delhi 110012, IndiaDivision of Plant Physiology, ICAR-Indian Agricultural Research Institute, New Delhi 110012, IndiaDivision of Plant Physiology, ICAR-Indian Agricultural Research Institute, New Delhi 110012, IndiaDivision of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi 110012, IndiaTo understand the physiological basis of tolerance to combined stresses to low phosphorus (P) and drought in mungbean (<i>Vigna</i><i>radiata</i> (L.) R. Wilczek), a diverse set of 100 accessions were evaluated in hydroponics at sufficient (250 μM) and low (3 μM) P and exposed to drought (dehydration) stress. The principal component analysis and ranking of accessions based on relative values revealed that IC280489, EC397142, IC76415, IC333090, IC507340, and IC121316 performed superior while IC119005, IC73401, IC488526, and IC325853 performed poorly in all treatments. Selected accessions were evaluated in soil under control (sufficient P, irrigated), low P (without P, irrigated), drought (sufficient P, withholding irrigation), and combined stress (low P, withholding irrigation). Under combined stress, a significant reduction in gas exchange traits (photosynthesis, stomatal conductance, transpiration, instantaneous water use efficiency) and P uptake in seed and shoot was observed under combined stress as compared to individual stresses. Among accessions, IC488526 was most sensitive while IC333090 and IC507340 exhibited tolerance to individual or combined stress. The water balance and low P adaptation traits like membrane stability index, relative water content, specific leaf weight, organic acid exudation, biomass, grain yield, and P uptake can be used as physiological markers to evaluate for agronomic performance. Accessions with considerable tolerance to low P and drought stress can be either used as ‘donors’ in Vigna breeding program or cultivated in areas with limited P and water availability or both.https://www.mdpi.com/2073-4395/11/1/99mungbeanlow phosphorusdrought stressorganic acid exudationphotosynthetic raterelative water content |
| spellingShingle | Surendra Kumar Meena Renu Pandey Sandeep Sharma Gayacharan Tarun Kumar Madan Pal Singh Harsh Kumar Dikshit Physiological Basis of Combined Stress Tolerance to Low Phosphorus and Drought in a Diverse Set of Mungbean Germplasm Agronomy mungbean low phosphorus drought stress organic acid exudation photosynthetic rate relative water content |
| title | Physiological Basis of Combined Stress Tolerance to Low Phosphorus and Drought in a Diverse Set of Mungbean Germplasm |
| title_full | Physiological Basis of Combined Stress Tolerance to Low Phosphorus and Drought in a Diverse Set of Mungbean Germplasm |
| title_fullStr | Physiological Basis of Combined Stress Tolerance to Low Phosphorus and Drought in a Diverse Set of Mungbean Germplasm |
| title_full_unstemmed | Physiological Basis of Combined Stress Tolerance to Low Phosphorus and Drought in a Diverse Set of Mungbean Germplasm |
| title_short | Physiological Basis of Combined Stress Tolerance to Low Phosphorus and Drought in a Diverse Set of Mungbean Germplasm |
| title_sort | physiological basis of combined stress tolerance to low phosphorus and drought in a diverse set of mungbean germplasm |
| topic | mungbean low phosphorus drought stress organic acid exudation photosynthetic rate relative water content |
| url | https://www.mdpi.com/2073-4395/11/1/99 |
| work_keys_str_mv | AT surendrakumarmeena physiologicalbasisofcombinedstresstolerancetolowphosphorusanddroughtinadiversesetofmungbeangermplasm AT renupandey physiologicalbasisofcombinedstresstolerancetolowphosphorusanddroughtinadiversesetofmungbeangermplasm AT sandeepsharma physiologicalbasisofcombinedstresstolerancetolowphosphorusanddroughtinadiversesetofmungbeangermplasm AT gayacharan physiologicalbasisofcombinedstresstolerancetolowphosphorusanddroughtinadiversesetofmungbeangermplasm AT tarunkumar physiologicalbasisofcombinedstresstolerancetolowphosphorusanddroughtinadiversesetofmungbeangermplasm AT madanpalsingh physiologicalbasisofcombinedstresstolerancetolowphosphorusanddroughtinadiversesetofmungbeangermplasm AT harshkumardikshit physiologicalbasisofcombinedstresstolerancetolowphosphorusanddroughtinadiversesetofmungbeangermplasm |