Bioluminescent imaging of Arabidopsis thaliana using an enhanced Nano-lantern luminescence reporter system.
Bioluminescent detection has become a powerful method that is used extensively in numerous areas in life science research. Given that fluorescence detection in plant cells is difficult owing to the autofluorescence of chlorophyll, the use of a luciferin-luciferase system should be effective in plant...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2020-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0227477&type=printable |
| _version_ | 1826574231495770112 |
|---|---|
| author | Yuichi Furuhata Ayako Sakai Tomi Murakami Akira Nagasaki Yoshio Kato |
| author_facet | Yuichi Furuhata Ayako Sakai Tomi Murakami Akira Nagasaki Yoshio Kato |
| author_sort | Yuichi Furuhata |
| collection | DOAJ |
| description | Bioluminescent detection has become a powerful method that is used extensively in numerous areas in life science research. Given that fluorescence detection in plant cells is difficult owing to the autofluorescence of chlorophyll, the use of a luciferin-luciferase system should be effective in plant biology. However, the suitable optical window for a luminescence system in plants remains unexplored. In this study, we sought to determine the optical window and optimal luciferase reporter system for terrestrial plant analyses using Arabidopsis thaliana as a model organism. We compared six different luciferase systems and found the green enhanced Nano-lantern (GeNL)-furimazine combination to be the optimal luciferase reporter. Spectral measurements of GeNL-furimazine showed that its luminescence peak falls within the range of optical transparency for chlorophyll and, therefore, enables greater penetration through a layer of cultured A. thaliana cells. Moreover, A. thaliana plants expressing GeNL with furimazine emitted strong luminescence, which could be detected even with the naked eye. Thus, the GeNL-furimazine combination should facilitate biological analyses of genes and cellular functions in A. thaliana and all other terrestrial plants. |
| first_indexed | 2024-12-20T19:26:22Z |
| format | Article |
| id | doaj.art-b4531a926e2e456ab82bea6ed0ee515b |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2025-03-14T12:55:31Z |
| publishDate | 2020-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-b4531a926e2e456ab82bea6ed0ee515b2025-03-02T05:33:22ZengPublic Library of Science (PLoS)PLoS ONE1932-62032020-01-01151e022747710.1371/journal.pone.0227477Bioluminescent imaging of Arabidopsis thaliana using an enhanced Nano-lantern luminescence reporter system.Yuichi FuruhataAyako SakaiTomi MurakamiAkira NagasakiYoshio KatoBioluminescent detection has become a powerful method that is used extensively in numerous areas in life science research. Given that fluorescence detection in plant cells is difficult owing to the autofluorescence of chlorophyll, the use of a luciferin-luciferase system should be effective in plant biology. However, the suitable optical window for a luminescence system in plants remains unexplored. In this study, we sought to determine the optical window and optimal luciferase reporter system for terrestrial plant analyses using Arabidopsis thaliana as a model organism. We compared six different luciferase systems and found the green enhanced Nano-lantern (GeNL)-furimazine combination to be the optimal luciferase reporter. Spectral measurements of GeNL-furimazine showed that its luminescence peak falls within the range of optical transparency for chlorophyll and, therefore, enables greater penetration through a layer of cultured A. thaliana cells. Moreover, A. thaliana plants expressing GeNL with furimazine emitted strong luminescence, which could be detected even with the naked eye. Thus, the GeNL-furimazine combination should facilitate biological analyses of genes and cellular functions in A. thaliana and all other terrestrial plants.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0227477&type=printable |
| spellingShingle | Yuichi Furuhata Ayako Sakai Tomi Murakami Akira Nagasaki Yoshio Kato Bioluminescent imaging of Arabidopsis thaliana using an enhanced Nano-lantern luminescence reporter system. PLoS ONE |
| title | Bioluminescent imaging of Arabidopsis thaliana using an enhanced Nano-lantern luminescence reporter system. |
| title_full | Bioluminescent imaging of Arabidopsis thaliana using an enhanced Nano-lantern luminescence reporter system. |
| title_fullStr | Bioluminescent imaging of Arabidopsis thaliana using an enhanced Nano-lantern luminescence reporter system. |
| title_full_unstemmed | Bioluminescent imaging of Arabidopsis thaliana using an enhanced Nano-lantern luminescence reporter system. |
| title_short | Bioluminescent imaging of Arabidopsis thaliana using an enhanced Nano-lantern luminescence reporter system. |
| title_sort | bioluminescent imaging of arabidopsis thaliana using an enhanced nano lantern luminescence reporter system |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0227477&type=printable |
| work_keys_str_mv | AT yuichifuruhata bioluminescentimagingofarabidopsisthalianausinganenhancednanolanternluminescencereportersystem AT ayakosakai bioluminescentimagingofarabidopsisthalianausinganenhancednanolanternluminescencereportersystem AT tomimurakami bioluminescentimagingofarabidopsisthalianausinganenhancednanolanternluminescencereportersystem AT akiranagasaki bioluminescentimagingofarabidopsisthalianausinganenhancednanolanternluminescencereportersystem AT yoshiokato bioluminescentimagingofarabidopsisthalianausinganenhancednanolanternluminescencereportersystem |