Dissection of AT-Hook Motif Nuclear-Localized Genes and Their Potential Functions in Peach Growth and Development
The AT-hook motif nuclear-localized (AHL) family members play key roles in plant biological processes via protein–protein and protein-DNA interactions. Here, 22 non-redundant <i>PpAHL</i> genes were identified and analyzed in peach (<i>Prunus persica</i>), one of economically...
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MDPI AG
2023-07-01
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| Online Access: | https://www.mdpi.com/1999-4907/14/7/1404 |
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| author | Jianlun Zhao Enkai Xu Qirui Wang |
| author_facet | Jianlun Zhao Enkai Xu Qirui Wang |
| author_sort | Jianlun Zhao |
| collection | DOAJ |
| description | The AT-hook motif nuclear-localized (AHL) family members play key roles in plant biological processes via protein–protein and protein-DNA interactions. Here, 22 non-redundant <i>PpAHL</i> genes were identified and analyzed in peach (<i>Prunus persica</i>), one of economically important non-timber forestry crops. The maximum-likelihood (ML) tree classified the <i>PpAHLs</i> into two clades (Clade-A and Clade-B) with three subfamilies: Type_I, Type_II, and Type_III. Exon–intron analysis exhibited that the <i>PpAHLs</i> from Type_I except one (<i>Prupe.1G530300.1</i>) lacked introns, and the <i>PpAHLs</i> from Type_II and Type_III gradually emerged with intron additions, indicating spatial expression patterns, evolutionarily distinct temporal patterns and, likely, neofunctionalization. Duplication event analysis suggested that <i>PpAHLs</i> in peach were mainly expanded through the large-scale duplication events. RNA-seq data showed that <i>PpAHLs</i> were induced by drought stress, and two genes (<i>Prupe.1G530300.1</i> and <i>Prupe.1G034400.1</i>) from Type_I AHLs were induced at all time points, indicating that they might play key roles in the response to drought stress in peach. The tissue-specific expression pattern of <i>PpAHLs</i> exhibited their biological functions in the development of these specific tissues. In addition, the transient overexpression of <i>Prupe.1G530300.1</i> and <i>Prupe.1G034400.1</i> resulted in significant changes in sugar content, suggesting that they may be positive regulators of sugar accumulation in peach fruits. Our study provided novel insights into the roles of <i>PpAHLs</i> in plant development, which was helpful for the functional analysis of peach and related woody fruit trees, and for formulating new strategies for further breeding. |
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| issn | 1999-4907 |
| language | English |
| last_indexed | 2024-03-11T01:03:59Z |
| publishDate | 2023-07-01 |
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| spelling | doaj.art-b456b309a7d64c0cbe7245b3346b394c2023-11-18T19:23:48ZengMDPI AGForests1999-49072023-07-01147140410.3390/f14071404Dissection of AT-Hook Motif Nuclear-Localized Genes and Their Potential Functions in Peach Growth and DevelopmentJianlun Zhao0Enkai Xu1Qirui Wang2College of Landscape Architecture and Art, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Landscape Architecture and Art, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Landscape Architecture and Art, Henan Agricultural University, Zhengzhou 450046, ChinaThe AT-hook motif nuclear-localized (AHL) family members play key roles in plant biological processes via protein–protein and protein-DNA interactions. Here, 22 non-redundant <i>PpAHL</i> genes were identified and analyzed in peach (<i>Prunus persica</i>), one of economically important non-timber forestry crops. The maximum-likelihood (ML) tree classified the <i>PpAHLs</i> into two clades (Clade-A and Clade-B) with three subfamilies: Type_I, Type_II, and Type_III. Exon–intron analysis exhibited that the <i>PpAHLs</i> from Type_I except one (<i>Prupe.1G530300.1</i>) lacked introns, and the <i>PpAHLs</i> from Type_II and Type_III gradually emerged with intron additions, indicating spatial expression patterns, evolutionarily distinct temporal patterns and, likely, neofunctionalization. Duplication event analysis suggested that <i>PpAHLs</i> in peach were mainly expanded through the large-scale duplication events. RNA-seq data showed that <i>PpAHLs</i> were induced by drought stress, and two genes (<i>Prupe.1G530300.1</i> and <i>Prupe.1G034400.1</i>) from Type_I AHLs were induced at all time points, indicating that they might play key roles in the response to drought stress in peach. The tissue-specific expression pattern of <i>PpAHLs</i> exhibited their biological functions in the development of these specific tissues. In addition, the transient overexpression of <i>Prupe.1G530300.1</i> and <i>Prupe.1G034400.1</i> resulted in significant changes in sugar content, suggesting that they may be positive regulators of sugar accumulation in peach fruits. Our study provided novel insights into the roles of <i>PpAHLs</i> in plant development, which was helpful for the functional analysis of peach and related woody fruit trees, and for formulating new strategies for further breeding.https://www.mdpi.com/1999-4907/14/7/1404peachnon-timber forestry cropsAHLexpressionduplicationexon–intron |
| spellingShingle | Jianlun Zhao Enkai Xu Qirui Wang Dissection of AT-Hook Motif Nuclear-Localized Genes and Their Potential Functions in Peach Growth and Development Forests peach non-timber forestry crops AHL expression duplication exon–intron |
| title | Dissection of AT-Hook Motif Nuclear-Localized Genes and Their Potential Functions in Peach Growth and Development |
| title_full | Dissection of AT-Hook Motif Nuclear-Localized Genes and Their Potential Functions in Peach Growth and Development |
| title_fullStr | Dissection of AT-Hook Motif Nuclear-Localized Genes and Their Potential Functions in Peach Growth and Development |
| title_full_unstemmed | Dissection of AT-Hook Motif Nuclear-Localized Genes and Their Potential Functions in Peach Growth and Development |
| title_short | Dissection of AT-Hook Motif Nuclear-Localized Genes and Their Potential Functions in Peach Growth and Development |
| title_sort | dissection of at hook motif nuclear localized genes and their potential functions in peach growth and development |
| topic | peach non-timber forestry crops AHL expression duplication exon–intron |
| url | https://www.mdpi.com/1999-4907/14/7/1404 |
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