| Summary: | <p>Abstract</p> <p>Background</p> <p><it>NPR1</it> is a gene of <it>Arabidopsis thaliana</it> required for the perception of salicylic acid. This perception triggers a defense response and negatively regulates the perception of jasmonates. Surprisingly, the application of methyl jasmonate also induces resistance, and <it>NPR1</it> is also suspected to be relevant. Since an allelic series of <it>npr1</it> was recently described, the behavior of these alleles was tested in response to methyl jasmonate.</p> <p>Results</p> <p>The response to methyl jasmonate of different <it>npr1s</it> alleles and <it>NPR1</it> paralogs null mutants was measured by the growth of a pathogen. We have also tested the subcellular localization of some npr1s, along with the protein-protein interactions that can be measured in yeast. The localization of the protein in <it>npr1</it> alleles does not affect the response to methyl jasmonate. In fact, <it>NPR1</it> is not required. The genes that are required in a redundant fashion are the <it>BOPs</it>. The <it>BOPs</it> are paralogs of <it>NPR1</it>, and they physically interact with the TGA family of transcription factors.</p> <p>Conclusions</p> <p>Some <it>npr1</it> alleles have a phenotype in this response likely because they are affecting the interaction between BOPs and TGAs, and these two families of proteins are responsible for the resistance induced by methyl jasmonate in wild type plants.</p>
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