Study of biochemical variability of potato cultivars by soluble protein, isoesterase, and isoperoxidase electrophoretic patterns

Biochemical variability between thirteen European and five Czech potato (Solanum tuberosum L.) cultivars grown in theCzechRepublic was studied by soluble protein, isoesterase, and isoperoxidase electrophoretic patterns. It was confirmed that cultivar differences in protein polymorphism can be reveal...

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Main Authors: J. Bárta, V. Čurn, J. Diviš
Format: Article
Language:English
Published: Czech Academy of Agricultural Sciences 2003-05-01
Series:Plant, Soil and Environment
Subjects:
Online Access:https://pse.agriculturejournals.cz/artkey/pse-200305-0007_study-of-biochemical-variability-of-potato-cultivars-by-soluble-protein-isoesterase-and-isoperoxidase-electro.php
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author J. Bárta
V. Čurn
J. Diviš
author_facet J. Bárta
V. Čurn
J. Diviš
author_sort J. Bárta
collection DOAJ
description Biochemical variability between thirteen European and five Czech potato (Solanum tuberosum L.) cultivars grown in theCzechRepublic was studied by soluble protein, isoesterase, and isoperoxidase electrophoretic patterns. It was confirmed that cultivar differences in protein polymorphism can be revealed by applied electrophoretic patterns. It was shown that the different character of protein and isozyme profiles required different approaches to their evaluation. For complex patterns such as electrophoretic soluble protein spectra, it is more convenient to use the evaluation of their absorbance profiles and for simpler profiles of isozymes the evaluation based on the presence or absence of a band in a definite position (simple matching) should be used. In spite of the complexity of tetraploid disposition of analysed cultivars, the results suggested higher similarity of profiles between relative cultivars and they also indicated the existence of higher similarity between cultivars from the same breeding firm.
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spelling doaj.art-b45b22c1dddf46ab9ef2037d6f77b0602023-02-23T03:44:52ZengCzech Academy of Agricultural SciencesPlant, Soil and Environment1214-11781805-93682003-05-0149523023610.17221/4118-PSEpse-200305-0007Study of biochemical variability of potato cultivars by soluble protein, isoesterase, and isoperoxidase electrophoretic patternsJ. Bárta0V. Čurn1J. Diviš2Faculty of Agriculture, University of South Bohemia in Èeské Budìjovice, Czech RepublicFaculty of Agriculture, University of South Bohemia in Èeské Budìjovice, Czech RepublicFaculty of Agriculture, University of South Bohemia in Èeské Budìjovice, Czech RepublicBiochemical variability between thirteen European and five Czech potato (Solanum tuberosum L.) cultivars grown in theCzechRepublic was studied by soluble protein, isoesterase, and isoperoxidase electrophoretic patterns. It was confirmed that cultivar differences in protein polymorphism can be revealed by applied electrophoretic patterns. It was shown that the different character of protein and isozyme profiles required different approaches to their evaluation. For complex patterns such as electrophoretic soluble protein spectra, it is more convenient to use the evaluation of their absorbance profiles and for simpler profiles of isozymes the evaluation based on the presence or absence of a band in a definite position (simple matching) should be used. In spite of the complexity of tetraploid disposition of analysed cultivars, the results suggested higher similarity of profiles between relative cultivars and they also indicated the existence of higher similarity between cultivars from the same breeding firm.https://pse.agriculturejournals.cz/artkey/pse-200305-0007_study-of-biochemical-variability-of-potato-cultivars-by-soluble-protein-isoesterase-and-isoperoxidase-electro.phppotatoescultivarselectrophoresisproteinsisoesterasesisoperoxidases
spellingShingle J. Bárta
V. Čurn
J. Diviš
Study of biochemical variability of potato cultivars by soluble protein, isoesterase, and isoperoxidase electrophoretic patterns
Plant, Soil and Environment
potatoes
cultivars
electrophoresis
proteins
isoesterases
isoperoxidases
title Study of biochemical variability of potato cultivars by soluble protein, isoesterase, and isoperoxidase electrophoretic patterns
title_full Study of biochemical variability of potato cultivars by soluble protein, isoesterase, and isoperoxidase electrophoretic patterns
title_fullStr Study of biochemical variability of potato cultivars by soluble protein, isoesterase, and isoperoxidase electrophoretic patterns
title_full_unstemmed Study of biochemical variability of potato cultivars by soluble protein, isoesterase, and isoperoxidase electrophoretic patterns
title_short Study of biochemical variability of potato cultivars by soluble protein, isoesterase, and isoperoxidase electrophoretic patterns
title_sort study of biochemical variability of potato cultivars by soluble protein isoesterase and isoperoxidase electrophoretic patterns
topic potatoes
cultivars
electrophoresis
proteins
isoesterases
isoperoxidases
url https://pse.agriculturejournals.cz/artkey/pse-200305-0007_study-of-biochemical-variability-of-potato-cultivars-by-soluble-protein-isoesterase-and-isoperoxidase-electro.php
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AT vcurn studyofbiochemicalvariabilityofpotatocultivarsbysolubleproteinisoesteraseandisoperoxidaseelectrophoreticpatterns
AT jdivis studyofbiochemicalvariabilityofpotatocultivarsbysolubleproteinisoesteraseandisoperoxidaseelectrophoreticpatterns