The emergence of Clostridioides difficile PCR ribotype 127 at a hospital in northeastern Taiwan
Background: Several studies have highlighted the incidence of Clostridioides difficile infections (CDIs) in Taiwan and certain ribotypes have been related to severe clinical diseases. A study was conducted to investigate the polymerase chain reaction (PCR) ribotypes and genetic relatedness of clinic...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-10-01
|
| Series: | Journal of Microbiology, Immunology and Infection |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1684118221002760 |
| _version_ | 1811340248234328064 |
|---|---|
| author | Bo-Yang Tsai Chun-Chih Chien Shu-Huan Huang Jun-Yuan Zheng Chih-Yu Hsu Yau-Sheng Tsai Yuan-Pin Hung Wen-Chien Ko Pei-Jane Tsai |
| author_facet | Bo-Yang Tsai Chun-Chih Chien Shu-Huan Huang Jun-Yuan Zheng Chih-Yu Hsu Yau-Sheng Tsai Yuan-Pin Hung Wen-Chien Ko Pei-Jane Tsai |
| author_sort | Bo-Yang Tsai |
| collection | DOAJ |
| description | Background: Several studies have highlighted the incidence of Clostridioides difficile infections (CDIs) in Taiwan and certain ribotypes have been related to severe clinical diseases. A study was conducted to investigate the polymerase chain reaction (PCR) ribotypes and genetic relatedness of clinical C. difficile strains collected from January 2009 to December 2015 at a hospital in northeastern Taiwan. Material and methods: A modified two-step typing algorithm for C. difficile was used by combining a modified 8-plex and 3′-truncated tcdA screening PCR. In addition, MLVA typing was adopted for investigation of bacterial clonality and transmission. Results: Among a total of 86 strains, 24 (28%) were nontoxigenic and 62 (72%) had both tcdA and tcdB (A + B+). No tcdA-negative and tcdB-positive (A−B+) strains were identified. Binary toxin (CDT)-producing (cdtA+/cdtB+) strains were started to be identified in 2013. The 21 (34%) A+B+ clinical strains with binary toxin and tcdC deletion were identified as RT127 strains, which contained both RT078-lineage markers and fluoroquinolone (FQ)-resistant mutations (Thr82Ile in gyrA). Multiple loci variable-number tandem repeat analysis (MLVA) for phylogenetic relatedness of RT127 strains indicated that 20 of 21 strains belonged to a clonal complex that was identical to a clinical strain collected from southern Taiwan in 2011, suggestive of a clonal expansion in Taiwan. Conclusion: A two-step typing method could rapidly confirm species identification and define the toxin gene profile of C. difficile isolates. The clonal expansion of RT127 strains in Taiwan indicates monitoring and surveillance of toxigenic C. difficile isolates from human, animal, and environment are critical to develop One Health prevention strategies. |
| first_indexed | 2024-04-13T18:39:11Z |
| format | Article |
| id | doaj.art-b45bb46a15fe4728b9625489c2266c4b |
| institution | Directory Open Access Journal |
| issn | 1684-1182 |
| language | English |
| last_indexed | 2024-04-13T18:39:11Z |
| publishDate | 2022-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Microbiology, Immunology and Infection |
| spelling | doaj.art-b45bb46a15fe4728b9625489c2266c4b2022-12-22T02:34:47ZengElsevierJournal of Microbiology, Immunology and Infection1684-11822022-10-01555896909The emergence of Clostridioides difficile PCR ribotype 127 at a hospital in northeastern TaiwanBo-Yang Tsai0Chun-Chih Chien1Shu-Huan Huang2Jun-Yuan Zheng3Chih-Yu Hsu4Yau-Sheng Tsai5Yuan-Pin Hung6Wen-Chien Ko7Pei-Jane Tsai8Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan, TaiwanDepartment of Laboratory Medicine, Chang Gung Memorial Hospital at Kaohsiung, Kaohsiung, TaiwanDepartment of Laboratory Medicine, Chang Gung Memorial Hospital at Kee-Lung, Keelung, TaiwanDivision of Infectious Diseases, Department of Internal Medicine, Chang Gung Memorial Hospital at Kee-Lung, Kee-Lung, TaiwanDepartment of Medical Laboratory Science and Biotechnology, National Cheng Kung University, Tainan, TaiwanInstitute of Clinical Medicine, National Cheng Kung University, Tainan, TaiwanDepartments of Internal Medicine, Tainan Hospital, Ministry of Health & Welfare, Tainan, Taiwan; Department of Internal Medicine, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, TaiwanDepartment of Internal Medicine, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan; Department of Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan; Corresponding author. Department of Internal Medicine, National Cheng Kung University Hospital, No. 138, Sheng Li Road, Tainan, 70403, Taiwan. Fax: +886 6 2752038.Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan, Taiwan; Department of Medical Laboratory Science and Biotechnology, National Cheng Kung University, Tainan, Taiwan; Center of Infectious Disease and Signaling Research, National Cheng Kung University, Tainan, Taiwan; Department of Pathology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan; Corresponding author. Department of Medical Laboratory Science and Biotechnology, College of Medicine, National Cheng Kung University, No. 1, University Road, Tainan, 70101, Taiwan. Fax: +886 6 2363956.Background: Several studies have highlighted the incidence of Clostridioides difficile infections (CDIs) in Taiwan and certain ribotypes have been related to severe clinical diseases. A study was conducted to investigate the polymerase chain reaction (PCR) ribotypes and genetic relatedness of clinical C. difficile strains collected from January 2009 to December 2015 at a hospital in northeastern Taiwan. Material and methods: A modified two-step typing algorithm for C. difficile was used by combining a modified 8-plex and 3′-truncated tcdA screening PCR. In addition, MLVA typing was adopted for investigation of bacterial clonality and transmission. Results: Among a total of 86 strains, 24 (28%) were nontoxigenic and 62 (72%) had both tcdA and tcdB (A + B+). No tcdA-negative and tcdB-positive (A−B+) strains were identified. Binary toxin (CDT)-producing (cdtA+/cdtB+) strains were started to be identified in 2013. The 21 (34%) A+B+ clinical strains with binary toxin and tcdC deletion were identified as RT127 strains, which contained both RT078-lineage markers and fluoroquinolone (FQ)-resistant mutations (Thr82Ile in gyrA). Multiple loci variable-number tandem repeat analysis (MLVA) for phylogenetic relatedness of RT127 strains indicated that 20 of 21 strains belonged to a clonal complex that was identical to a clinical strain collected from southern Taiwan in 2011, suggestive of a clonal expansion in Taiwan. Conclusion: A two-step typing method could rapidly confirm species identification and define the toxin gene profile of C. difficile isolates. The clonal expansion of RT127 strains in Taiwan indicates monitoring and surveillance of toxigenic C. difficile isolates from human, animal, and environment are critical to develop One Health prevention strategies.http://www.sciencedirect.com/science/article/pii/S1684118221002760Clostridioides difficileClusterHypertoxigenicMLVART127 |
| spellingShingle | Bo-Yang Tsai Chun-Chih Chien Shu-Huan Huang Jun-Yuan Zheng Chih-Yu Hsu Yau-Sheng Tsai Yuan-Pin Hung Wen-Chien Ko Pei-Jane Tsai The emergence of Clostridioides difficile PCR ribotype 127 at a hospital in northeastern Taiwan Journal of Microbiology, Immunology and Infection Clostridioides difficile Cluster Hypertoxigenic MLVA RT127 |
| title | The emergence of Clostridioides difficile PCR ribotype 127 at a hospital in northeastern Taiwan |
| title_full | The emergence of Clostridioides difficile PCR ribotype 127 at a hospital in northeastern Taiwan |
| title_fullStr | The emergence of Clostridioides difficile PCR ribotype 127 at a hospital in northeastern Taiwan |
| title_full_unstemmed | The emergence of Clostridioides difficile PCR ribotype 127 at a hospital in northeastern Taiwan |
| title_short | The emergence of Clostridioides difficile PCR ribotype 127 at a hospital in northeastern Taiwan |
| title_sort | emergence of clostridioides difficile pcr ribotype 127 at a hospital in northeastern taiwan |
| topic | Clostridioides difficile Cluster Hypertoxigenic MLVA RT127 |
| url | http://www.sciencedirect.com/science/article/pii/S1684118221002760 |
| work_keys_str_mv | AT boyangtsai theemergenceofclostridioidesdifficilepcrribotype127atahospitalinnortheasterntaiwan AT chunchihchien theemergenceofclostridioidesdifficilepcrribotype127atahospitalinnortheasterntaiwan AT shuhuanhuang theemergenceofclostridioidesdifficilepcrribotype127atahospitalinnortheasterntaiwan AT junyuanzheng theemergenceofclostridioidesdifficilepcrribotype127atahospitalinnortheasterntaiwan AT chihyuhsu theemergenceofclostridioidesdifficilepcrribotype127atahospitalinnortheasterntaiwan AT yaushengtsai theemergenceofclostridioidesdifficilepcrribotype127atahospitalinnortheasterntaiwan AT yuanpinhung theemergenceofclostridioidesdifficilepcrribotype127atahospitalinnortheasterntaiwan AT wenchienko theemergenceofclostridioidesdifficilepcrribotype127atahospitalinnortheasterntaiwan AT peijanetsai theemergenceofclostridioidesdifficilepcrribotype127atahospitalinnortheasterntaiwan AT boyangtsai emergenceofclostridioidesdifficilepcrribotype127atahospitalinnortheasterntaiwan AT chunchihchien emergenceofclostridioidesdifficilepcrribotype127atahospitalinnortheasterntaiwan AT shuhuanhuang emergenceofclostridioidesdifficilepcrribotype127atahospitalinnortheasterntaiwan AT junyuanzheng emergenceofclostridioidesdifficilepcrribotype127atahospitalinnortheasterntaiwan AT chihyuhsu emergenceofclostridioidesdifficilepcrribotype127atahospitalinnortheasterntaiwan AT yaushengtsai emergenceofclostridioidesdifficilepcrribotype127atahospitalinnortheasterntaiwan AT yuanpinhung emergenceofclostridioidesdifficilepcrribotype127atahospitalinnortheasterntaiwan AT wenchienko emergenceofclostridioidesdifficilepcrribotype127atahospitalinnortheasterntaiwan AT peijanetsai emergenceofclostridioidesdifficilepcrribotype127atahospitalinnortheasterntaiwan |