Bioremediation of Fluorophenols by Glycosylation with Immobilized Marine Microalga Amphidinium Crassum
Fluorophenols are used as agrochemicals and released into environment as pollutants. Cultured marine microalga Amphidinium crassum ( Gymnodinium ) glucosylated 2-fluorophenol ( 1 ), 3-fluorophenol ( 2 ), and 4-fluorophenol ( 3 ) to the corresponding β-D-glucosides, ie, 2-fluorophenyl β-D-glucoside (...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2010-01-01
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| Series: | Environmental Health Insights |
| Online Access: | https://doi.org/10.4137/EHI.S5392 |
| _version_ | 1818623024827465728 |
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| author | Kei Shimoda Hiroki Hamada |
| author_facet | Kei Shimoda Hiroki Hamada |
| author_sort | Kei Shimoda |
| collection | DOAJ |
| description | Fluorophenols are used as agrochemicals and released into environment as pollutants. Cultured marine microalga Amphidinium crassum ( Gymnodinium ) glucosylated 2-fluorophenol ( 1 ), 3-fluorophenol ( 2 ), and 4-fluorophenol ( 3 ) to the corresponding β-D-glucosides, ie, 2-fluorophenyl β-D-glucoside ( 4 , 60 μg/g cells), 3-fluorophenyl β-D-glucoside ( 5 , 20 μg/g cells), and 4-fluorophenyl β-D-glucoside ( 6 , 40 μg/g cells). On the other hand, 2-, 3-, and 4-fluorophenols were efficiently converted by immobilized A. crassum in sodium alginate gel to give their β-D-glucosides in higher yields ( 4 : 140 μg/g cells; 5 : 60 μg/g cells; 6 : 100 μg/g cells). In repetitive batch use, the immobilized cells of A. crassum maintained the potential for the glucosylation of the substrate fluorophenol after 5 times of usage. |
| first_indexed | 2024-12-16T18:34:29Z |
| format | Article |
| id | doaj.art-b45c3115fd514f1090fe1a7c52f899fc |
| institution | Directory Open Access Journal |
| issn | 1178-6302 |
| language | English |
| last_indexed | 2024-12-16T18:34:29Z |
| publishDate | 2010-01-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Environmental Health Insights |
| spelling | doaj.art-b45c3115fd514f1090fe1a7c52f899fc2022-12-21T22:21:13ZengSAGE PublishingEnvironmental Health Insights1178-63022010-01-01410.4137/EHI.S5392Bioremediation of Fluorophenols by Glycosylation with Immobilized Marine Microalga Amphidinium CrassumKei Shimoda0Hiroki Hamada1Department of Chemistry, Faculty of Medicine, Oita University, 1-1 Hasama-machi, Oita 879-5593, Japan.Department of Life Science, Faculty of Science, Okayama University of Science, 1-1 Ridai-cho, Kita-ku, Okayama 700-0005, Japan.Fluorophenols are used as agrochemicals and released into environment as pollutants. Cultured marine microalga Amphidinium crassum ( Gymnodinium ) glucosylated 2-fluorophenol ( 1 ), 3-fluorophenol ( 2 ), and 4-fluorophenol ( 3 ) to the corresponding β-D-glucosides, ie, 2-fluorophenyl β-D-glucoside ( 4 , 60 μg/g cells), 3-fluorophenyl β-D-glucoside ( 5 , 20 μg/g cells), and 4-fluorophenyl β-D-glucoside ( 6 , 40 μg/g cells). On the other hand, 2-, 3-, and 4-fluorophenols were efficiently converted by immobilized A. crassum in sodium alginate gel to give their β-D-glucosides in higher yields ( 4 : 140 μg/g cells; 5 : 60 μg/g cells; 6 : 100 μg/g cells). In repetitive batch use, the immobilized cells of A. crassum maintained the potential for the glucosylation of the substrate fluorophenol after 5 times of usage.https://doi.org/10.4137/EHI.S5392 |
| spellingShingle | Kei Shimoda Hiroki Hamada Bioremediation of Fluorophenols by Glycosylation with Immobilized Marine Microalga Amphidinium Crassum Environmental Health Insights |
| title | Bioremediation of Fluorophenols by Glycosylation with Immobilized Marine Microalga Amphidinium Crassum |
| title_full | Bioremediation of Fluorophenols by Glycosylation with Immobilized Marine Microalga Amphidinium Crassum |
| title_fullStr | Bioremediation of Fluorophenols by Glycosylation with Immobilized Marine Microalga Amphidinium Crassum |
| title_full_unstemmed | Bioremediation of Fluorophenols by Glycosylation with Immobilized Marine Microalga Amphidinium Crassum |
| title_short | Bioremediation of Fluorophenols by Glycosylation with Immobilized Marine Microalga Amphidinium Crassum |
| title_sort | bioremediation of fluorophenols by glycosylation with immobilized marine microalga amphidinium crassum |
| url | https://doi.org/10.4137/EHI.S5392 |
| work_keys_str_mv | AT keishimoda bioremediationoffluorophenolsbyglycosylationwithimmobilizedmarinemicroalgaamphidiniumcrassum AT hirokihamada bioremediationoffluorophenolsbyglycosylationwithimmobilizedmarinemicroalgaamphidiniumcrassum |