Legume-Rhizobium Strain Specificity Enhances Nutrition and Nitrogen Fixation in Faba Bean (<i>Vicia faba</i> L.)
This study reports the effectiveness of some selected rhizobium strains in enhancing nitrogen fixation and nutrient uptake in <i>Vicia faba</i> L. Multi-location field experiments were conducted for two years (2016 and 2017) using a split-plot in randomized complete block design. Treatme...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-06-01
|
| Series: | Agronomy |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4395/10/6/826 |
| _version_ | 1797417096949268480 |
|---|---|
| author | Bayou Bunkura Allito Nana Ewusi-Mensah Vincent Logah |
| author_facet | Bayou Bunkura Allito Nana Ewusi-Mensah Vincent Logah |
| author_sort | Bayou Bunkura Allito |
| collection | DOAJ |
| description | This study reports the effectiveness of some selected rhizobium strains in enhancing nitrogen fixation and nutrient uptake in <i>Vicia faba</i> L. Multi-location field experiments were conducted for two years (2016 and 2017) using a split-plot in randomized complete block design. Treatments comprised six rhizobium strains as the main plot factor and three varieties of <i>Vicia faba</i> as the sub-plot factor. Non-inoculated plants with or without N fertilizer served as +N and −N controls, respectively. Peat carrier-based inoculant of each strain was applied at the rate of 10 g kg<sup>−1</sup> seed. Data on nodulation were taken at the late-flowering stage, whereas nitrogen and phosphorus concentrations in plant parts were analyzed at physiological maturity. The total nitrogen difference method was employed to quantify nitrogen fixation. Location x rhizobium strain x variety interaction had a significant effect on nodule dry weight plant<sup>−1</sup>. Rhizobium strains significantly enhanced nodulation, nitrogen fixation, nutrient uptake and soil nitrogen balance. Inoculation with NSFBR-12 and NSFBR-15 resulted in the highest nitrogen fixed, nutrient uptake and soil nitrogen balance. <i>Vicia faba</i> inoculated with the two top performing strains, NSFBR-12 and NSFBR-15 fixed respectively 87.7% and 85.5% of the total nitrogen uptake. Non-inoculated plants fulfilled proportionately more of the total nitrogen uptake through nitrogen derived from the soil rather than fixed nitrogen. Soil available phosphorus and pH had appreciable influences on nitrogen and phosphorus uptake of inoculated <i>Vicia faba</i>. Inoculation with competitive and effective rhizobium strains can improve soil nitrogen balance, nitrogen fixation and nutrient uptake of <i>Vicia faba.</i> |
| first_indexed | 2024-03-09T06:13:57Z |
| format | Article |
| id | doaj.art-b45ea707ce7c4d56a1c73c79e6c84df0 |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-09T06:13:57Z |
| publishDate | 2020-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-b45ea707ce7c4d56a1c73c79e6c84df02023-12-03T11:55:58ZengMDPI AGAgronomy2073-43952020-06-0110682610.3390/agronomy10060826Legume-Rhizobium Strain Specificity Enhances Nutrition and Nitrogen Fixation in Faba Bean (<i>Vicia faba</i> L.)Bayou Bunkura Allito0Nana Ewusi-Mensah1Vincent Logah2Department of Plant and Horticultural Science, Hawassa University College of Agriculture, Hawassa P.O. Box 05, EthiopiaDepartment of Crop and Soil Sciences, Kwame Nkrumah University of Science and Technology, Kumasi AK-039, GhanaDepartment of Crop and Soil Sciences, Kwame Nkrumah University of Science and Technology, Kumasi AK-039, GhanaThis study reports the effectiveness of some selected rhizobium strains in enhancing nitrogen fixation and nutrient uptake in <i>Vicia faba</i> L. Multi-location field experiments were conducted for two years (2016 and 2017) using a split-plot in randomized complete block design. Treatments comprised six rhizobium strains as the main plot factor and three varieties of <i>Vicia faba</i> as the sub-plot factor. Non-inoculated plants with or without N fertilizer served as +N and −N controls, respectively. Peat carrier-based inoculant of each strain was applied at the rate of 10 g kg<sup>−1</sup> seed. Data on nodulation were taken at the late-flowering stage, whereas nitrogen and phosphorus concentrations in plant parts were analyzed at physiological maturity. The total nitrogen difference method was employed to quantify nitrogen fixation. Location x rhizobium strain x variety interaction had a significant effect on nodule dry weight plant<sup>−1</sup>. Rhizobium strains significantly enhanced nodulation, nitrogen fixation, nutrient uptake and soil nitrogen balance. Inoculation with NSFBR-12 and NSFBR-15 resulted in the highest nitrogen fixed, nutrient uptake and soil nitrogen balance. <i>Vicia faba</i> inoculated with the two top performing strains, NSFBR-12 and NSFBR-15 fixed respectively 87.7% and 85.5% of the total nitrogen uptake. Non-inoculated plants fulfilled proportionately more of the total nitrogen uptake through nitrogen derived from the soil rather than fixed nitrogen. Soil available phosphorus and pH had appreciable influences on nitrogen and phosphorus uptake of inoculated <i>Vicia faba</i>. Inoculation with competitive and effective rhizobium strains can improve soil nitrogen balance, nitrogen fixation and nutrient uptake of <i>Vicia faba.</i>https://www.mdpi.com/2073-4395/10/6/826inoculationnodulationnutrient uptakerhizobium strain<i>Vicia faba</i> |
| spellingShingle | Bayou Bunkura Allito Nana Ewusi-Mensah Vincent Logah Legume-Rhizobium Strain Specificity Enhances Nutrition and Nitrogen Fixation in Faba Bean (<i>Vicia faba</i> L.) Agronomy inoculation nodulation nutrient uptake rhizobium strain <i>Vicia faba</i> |
| title | Legume-Rhizobium Strain Specificity Enhances Nutrition and Nitrogen Fixation in Faba Bean (<i>Vicia faba</i> L.) |
| title_full | Legume-Rhizobium Strain Specificity Enhances Nutrition and Nitrogen Fixation in Faba Bean (<i>Vicia faba</i> L.) |
| title_fullStr | Legume-Rhizobium Strain Specificity Enhances Nutrition and Nitrogen Fixation in Faba Bean (<i>Vicia faba</i> L.) |
| title_full_unstemmed | Legume-Rhizobium Strain Specificity Enhances Nutrition and Nitrogen Fixation in Faba Bean (<i>Vicia faba</i> L.) |
| title_short | Legume-Rhizobium Strain Specificity Enhances Nutrition and Nitrogen Fixation in Faba Bean (<i>Vicia faba</i> L.) |
| title_sort | legume rhizobium strain specificity enhances nutrition and nitrogen fixation in faba bean i vicia faba i l |
| topic | inoculation nodulation nutrient uptake rhizobium strain <i>Vicia faba</i> |
| url | https://www.mdpi.com/2073-4395/10/6/826 |
| work_keys_str_mv | AT bayoubunkuraallito legumerhizobiumstrainspecificityenhancesnutritionandnitrogenfixationinfababeaniviciafabail AT nanaewusimensah legumerhizobiumstrainspecificityenhancesnutritionandnitrogenfixationinfababeaniviciafabail AT vincentlogah legumerhizobiumstrainspecificityenhancesnutritionandnitrogenfixationinfababeaniviciafabail |