Role of L-lactate as an energy substrate in primary rat podocytes under physiological and glucose deprivation conditions
Lactate has long been acknowledged to be a metabolic waste product, but it has more recently been found as a fuel energy source in mammalian cells. Podocytes are an important component of the glomerular filter, and their role in maintaining the structural integrity of this structure was established....
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | European Journal of Cell Biology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0171933523000134 |
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| author | Maria Szrejder Marlena Typiak Piotr Pikul Irena Audzeyenka Patrycja Rachubik Dorota Rogacka Magdalena Narajczyk Agnieszka Piwkowska |
| author_facet | Maria Szrejder Marlena Typiak Piotr Pikul Irena Audzeyenka Patrycja Rachubik Dorota Rogacka Magdalena Narajczyk Agnieszka Piwkowska |
| author_sort | Maria Szrejder |
| collection | DOAJ |
| description | Lactate has long been acknowledged to be a metabolic waste product, but it has more recently been found as a fuel energy source in mammalian cells. Podocytes are an important component of the glomerular filter, and their role in maintaining the structural integrity of this structure was established. These cells rely on a constant energy supply and reservoir. The utilization of alternative energy substrates to preserve energetic homeostasis is a subject of extensive research, and lactate appears to be one such candidate. Therefore, we investigated the role of lactate as an energy substrate and characterize the lactate transport system in cultured rat podocytes during sufficient and insufficient glucose supplies. The present study, for the first time, demonstrated the presence of lactate transporters in podocytes. Moreover, we observed modified the amount of these transporters in response to limited glucose availability and after l-lactate supplementation. Simultaneously, exposure to l-lactate preserved cell survival during insufficient glucose supply. Interestingly, during glucose deprivation, lactate exposure allowed the steady flow of glycolysis and prevented glycogen reserves depletion. Summarizing, podocytes utilize lactate as an energy substrate and possess a developed system that controls lactate homeostasis, suggesting that it plays an essential role in podocyte metabolism, especially during fluctuations of energy availability. |
| first_indexed | 2024-03-13T04:14:54Z |
| format | Article |
| id | doaj.art-b45f3704119d4ad8a356a2a1b2b58f89 |
| institution | Directory Open Access Journal |
| issn | 0171-9335 |
| language | English |
| last_indexed | 2024-03-13T04:14:54Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | European Journal of Cell Biology |
| spelling | doaj.art-b45f3704119d4ad8a356a2a1b2b58f892023-06-21T06:50:54ZengElsevierEuropean Journal of Cell Biology0171-93352023-06-011022151298Role of L-lactate as an energy substrate in primary rat podocytes under physiological and glucose deprivation conditionsMaria Szrejder0Marlena Typiak1Piotr Pikul2Irena Audzeyenka3Patrycja Rachubik4Dorota Rogacka5Magdalena Narajczyk6Agnieszka Piwkowska7Mossakowski Medical Research Institute, Polish Academy of Sciences, Laboratory of Molecular and Cellular Nephrology, Gdańsk, Poland; Corresponding author.Mossakowski Medical Research Institute, Polish Academy of Sciences, Laboratory of Molecular and Cellular Nephrology, Gdańsk, Poland; University of Gdansk, Faculty of Biology, Gdansk, PolandMossakowski Medical Research Institute, Polish Academy of Sciences, Laboratory of Molecular and Cellular Nephrology, Gdańsk, PolandMossakowski Medical Research Institute, Polish Academy of Sciences, Laboratory of Molecular and Cellular Nephrology, Gdańsk, Poland; University of Gdansk, Faculty of Chemistry, Gdańsk, PolandMossakowski Medical Research Institute, Polish Academy of Sciences, Laboratory of Molecular and Cellular Nephrology, Gdańsk, PolandMossakowski Medical Research Institute, Polish Academy of Sciences, Laboratory of Molecular and Cellular Nephrology, Gdańsk, Poland; University of Gdansk, Faculty of Chemistry, Gdańsk, PolandUniversity of Gdansk, Faculty of Biology, Gdansk, PolandMossakowski Medical Research Institute, Polish Academy of Sciences, Laboratory of Molecular and Cellular Nephrology, Gdańsk, Poland; University of Gdansk, Faculty of Chemistry, Gdańsk, PolandLactate has long been acknowledged to be a metabolic waste product, but it has more recently been found as a fuel energy source in mammalian cells. Podocytes are an important component of the glomerular filter, and their role in maintaining the structural integrity of this structure was established. These cells rely on a constant energy supply and reservoir. The utilization of alternative energy substrates to preserve energetic homeostasis is a subject of extensive research, and lactate appears to be one such candidate. Therefore, we investigated the role of lactate as an energy substrate and characterize the lactate transport system in cultured rat podocytes during sufficient and insufficient glucose supplies. The present study, for the first time, demonstrated the presence of lactate transporters in podocytes. Moreover, we observed modified the amount of these transporters in response to limited glucose availability and after l-lactate supplementation. Simultaneously, exposure to l-lactate preserved cell survival during insufficient glucose supply. Interestingly, during glucose deprivation, lactate exposure allowed the steady flow of glycolysis and prevented glycogen reserves depletion. Summarizing, podocytes utilize lactate as an energy substrate and possess a developed system that controls lactate homeostasis, suggesting that it plays an essential role in podocyte metabolism, especially during fluctuations of energy availability.http://www.sciencedirect.com/science/article/pii/S0171933523000134PodocytesLactateLactate transportersGlycolysisEnergy metabolism |
| spellingShingle | Maria Szrejder Marlena Typiak Piotr Pikul Irena Audzeyenka Patrycja Rachubik Dorota Rogacka Magdalena Narajczyk Agnieszka Piwkowska Role of L-lactate as an energy substrate in primary rat podocytes under physiological and glucose deprivation conditions European Journal of Cell Biology Podocytes Lactate Lactate transporters Glycolysis Energy metabolism |
| title | Role of L-lactate as an energy substrate in primary rat podocytes under physiological and glucose deprivation conditions |
| title_full | Role of L-lactate as an energy substrate in primary rat podocytes under physiological and glucose deprivation conditions |
| title_fullStr | Role of L-lactate as an energy substrate in primary rat podocytes under physiological and glucose deprivation conditions |
| title_full_unstemmed | Role of L-lactate as an energy substrate in primary rat podocytes under physiological and glucose deprivation conditions |
| title_short | Role of L-lactate as an energy substrate in primary rat podocytes under physiological and glucose deprivation conditions |
| title_sort | role of l lactate as an energy substrate in primary rat podocytes under physiological and glucose deprivation conditions |
| topic | Podocytes Lactate Lactate transporters Glycolysis Energy metabolism |
| url | http://www.sciencedirect.com/science/article/pii/S0171933523000134 |
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