Evaluation of ruminal fermentation of ensiled fruit byproducts and their potential for feed use
Objective Ensiling of tannin-rich fruit byproducts (FB) involves quantitative and qualitative changes in the tannins, which would consequently change the rumen fermentation characteristics. This study aimed to evaluate whether ensiled FBs are effective in mitigating methane emission from ruminants b...
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| Format: | Article |
| Language: | English |
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Asian-Australasian Association of Animal Production Societies
2019-01-01
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| Series: | Asian-Australasian Journal of Animal Sciences |
| Subjects: | |
| Online Access: | http://www.ajas.info/upload/pdf/ajas-18-0282.pdf |
| _version_ | 1818933957034508288 |
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| author | Shimaa A Mousa Pradeep K. Malik Atul P. Kolte Raghavendra Bhatta Shigemitsu Kasuga Yutaka Uyeno |
| author_facet | Shimaa A Mousa Pradeep K. Malik Atul P. Kolte Raghavendra Bhatta Shigemitsu Kasuga Yutaka Uyeno |
| author_sort | Shimaa A Mousa |
| collection | DOAJ |
| description | Objective Ensiling of tannin-rich fruit byproducts (FB) involves quantitative and qualitative changes in the tannins, which would consequently change the rumen fermentation characteristics. This study aimed to evaluate whether ensiled FBs are effective in mitigating methane emission from ruminants by conducting in vitro assessments. Methods Fruit byproducts (grape pomace, wild grape pomace, and persimmon skin) were collected and subjected to four-week ensiling by Lactobacillus buchneri inoculant. A defined feed component with or without FB samples (both fresh and ensiled material) were subjected to in vitro anaerobic culturing using rumen fluid sampled from beef cattle, and the fermentation parameters and microbial populations were monitored. Results Reduced methane production and a proportional change in total volatile fatty acids (especially enhanced propionate proportion) was noted in bottles containing the FBs compared with that in the control (without FB). In addition, we found lower gene copy number of archaeal 16S rRNA and considerably higher levels of one of the major fibrolytic bacteria (Fibrobacter succinogenes) in the bottles containing FBs than in the control, particularly, when it was included in a forage-based feed. However, in the following cultivation experiment, we observed that FBs failed to exhibit a significant difference in methane production with or without polyethylene glycol, implying that tannins in the FBs may not be responsible for the mitigation of methane generation. Conclusion The results of the in vitro cultivation experiments indicated that not only the composition but also ensiling of FBs affected rumen fermentation patterns and the degree of methane generation. This is primarily because of the compositional changes in the fibrous fraction during ensiling as well as the presence of readily fermented substrates, whereas tannins in these FBs seemed to have little effect on the ruminal fermentation kinetics. |
| first_indexed | 2024-12-20T04:56:38Z |
| format | Article |
| id | doaj.art-b45faf87544744f5a04380e40b9cf029 |
| institution | Directory Open Access Journal |
| issn | 1011-2367 1976-5517 |
| language | English |
| last_indexed | 2024-12-20T04:56:38Z |
| publishDate | 2019-01-01 |
| publisher | Asian-Australasian Association of Animal Production Societies |
| record_format | Article |
| series | Asian-Australasian Journal of Animal Sciences |
| spelling | doaj.art-b45faf87544744f5a04380e40b9cf0292022-12-21T19:52:42ZengAsian-Australasian Association of Animal Production SocietiesAsian-Australasian Journal of Animal Sciences1011-23671976-55172019-01-0132110310910.5713/ajas.18.028224154Evaluation of ruminal fermentation of ensiled fruit byproducts and their potential for feed useShimaa A Mousa0Pradeep K. Malik1Atul P. Kolte2Raghavendra Bhatta3Shigemitsu Kasuga4Yutaka Uyeno5 Graduate School of Science and Technology, Shinshu University, Minamiminowa 3994598, Japan Energy Metabolism Laboratory, ICAR-National Institute of Animal Nutrition and Physiology, Bengaluru 560030, India Energy Metabolism Laboratory, ICAR-National Institute of Animal Nutrition and Physiology, Bengaluru 560030, India Energy Metabolism Laboratory, ICAR-National Institute of Animal Nutrition and Physiology, Bengaluru 560030, India Graduate School of Science and Technology, Shinshu University, Minamiminowa 3994598, Japan Graduate School of Science and Technology, Shinshu University, Minamiminowa 3994598, JapanObjective Ensiling of tannin-rich fruit byproducts (FB) involves quantitative and qualitative changes in the tannins, which would consequently change the rumen fermentation characteristics. This study aimed to evaluate whether ensiled FBs are effective in mitigating methane emission from ruminants by conducting in vitro assessments. Methods Fruit byproducts (grape pomace, wild grape pomace, and persimmon skin) were collected and subjected to four-week ensiling by Lactobacillus buchneri inoculant. A defined feed component with or without FB samples (both fresh and ensiled material) were subjected to in vitro anaerobic culturing using rumen fluid sampled from beef cattle, and the fermentation parameters and microbial populations were monitored. Results Reduced methane production and a proportional change in total volatile fatty acids (especially enhanced propionate proportion) was noted in bottles containing the FBs compared with that in the control (without FB). In addition, we found lower gene copy number of archaeal 16S rRNA and considerably higher levels of one of the major fibrolytic bacteria (Fibrobacter succinogenes) in the bottles containing FBs than in the control, particularly, when it was included in a forage-based feed. However, in the following cultivation experiment, we observed that FBs failed to exhibit a significant difference in methane production with or without polyethylene glycol, implying that tannins in the FBs may not be responsible for the mitigation of methane generation. Conclusion The results of the in vitro cultivation experiments indicated that not only the composition but also ensiling of FBs affected rumen fermentation patterns and the degree of methane generation. This is primarily because of the compositional changes in the fibrous fraction during ensiling as well as the presence of readily fermented substrates, whereas tannins in these FBs seemed to have little effect on the ruminal fermentation kinetics.http://www.ajas.info/upload/pdf/ajas-18-0282.pdfFruit ByproductsEnsilingMethaneTannins |
| spellingShingle | Shimaa A Mousa Pradeep K. Malik Atul P. Kolte Raghavendra Bhatta Shigemitsu Kasuga Yutaka Uyeno Evaluation of ruminal fermentation of ensiled fruit byproducts and their potential for feed use Asian-Australasian Journal of Animal Sciences Fruit Byproducts Ensiling Methane Tannins |
| title | Evaluation of ruminal fermentation of ensiled fruit byproducts and their potential for feed use |
| title_full | Evaluation of ruminal fermentation of ensiled fruit byproducts and their potential for feed use |
| title_fullStr | Evaluation of ruminal fermentation of ensiled fruit byproducts and their potential for feed use |
| title_full_unstemmed | Evaluation of ruminal fermentation of ensiled fruit byproducts and their potential for feed use |
| title_short | Evaluation of ruminal fermentation of ensiled fruit byproducts and their potential for feed use |
| title_sort | evaluation of ruminal fermentation of ensiled fruit byproducts and their potential for feed use |
| topic | Fruit Byproducts Ensiling Methane Tannins |
| url | http://www.ajas.info/upload/pdf/ajas-18-0282.pdf |
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