Ectopic Expression of a Salt-Inducible Gene, <i>LcSAIN3</i>, from Sheepgrass Improves Seed Germination and Seedling Growth under Salt Stress in Arabidopsis
Sheepgrass is a perennial native grass species in China, and it can tolerate high levels of salt stress with an aggressive and vigorous rhizome system. Many salt-stress-responsive genes have been identified in sheepgrass. In this study, we report the cloning and characterization of a novel salt-indu...
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2021-12-01
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| author | Xiaoxia Li Weiguang Yang Junting Jia Pincang Zhao Dongmei Qi Shuangyan Chen Li Cheng Liqin Cheng Gongshe Liu |
| author_facet | Xiaoxia Li Weiguang Yang Junting Jia Pincang Zhao Dongmei Qi Shuangyan Chen Li Cheng Liqin Cheng Gongshe Liu |
| author_sort | Xiaoxia Li |
| collection | DOAJ |
| description | Sheepgrass is a perennial native grass species in China, and it can tolerate high levels of salt stress with an aggressive and vigorous rhizome system. Many salt-stress-responsive genes have been identified in sheepgrass. In this study, we report the cloning and characterization of a novel salt-induced gene, <i>LcSAIN3</i> (<i>Leymus chinensis</i> salt-induced 3), from sheepgrass. Expression analysis confirmed that <i>LcSAIN3</i> was induced by PEG, ABA, and salt treatments, and the expression of <i>LcSAIN3</i> was significantly increased in salt-tolerant germplasms under salt treatment. Subcellular localization analysis indicated that the GFP-LcSAIN3 protein was mainly localized in the chloroplasts. The heterologous expression of <i>LcSAIN3</i> in Arabidopsis increased the seed germination rate of transgenic plants under salt, ABA, and mannitol treatments. The seedling survival rate, plant height, and fresh weight of the transgenic plants were higher than those of WT plants under salt stress. The overexpression of <i>LcSAIN3</i> caused a relatively high accumulation of free proline, enhanced SOD activity, and led to the upregulation of several stress-responsive genes such as <i>AtRD26</i>, <i>AtRD29B</i>, <i>AtSOS1,</i> and <i>AtP5CS1</i>. These results suggest that <i>LcSAIN3</i> could be a potential target for molecular breeding to improve plants’ salt tolerance. |
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| spelling | doaj.art-b4604ec75bf74cd6a7b115c9046bef942023-11-23T08:31:25ZengMDPI AGGenes2073-44252021-12-011212199410.3390/genes12121994Ectopic Expression of a Salt-Inducible Gene, <i>LcSAIN3</i>, from Sheepgrass Improves Seed Germination and Seedling Growth under Salt Stress in ArabidopsisXiaoxia Li0Weiguang Yang1Junting Jia2Pincang Zhao3Dongmei Qi4Shuangyan Chen5Li Cheng6Liqin Cheng7Gongshe Liu8Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, ChinaCollege of Animal Science and Veterinary Medicine, Helongjiang Bayi Agricultural University, Daqing 163000, ChinaGuangdong Provincial Key Laboratory for Crop Germplasm Resources Preservation and Utilization, Agro-Biological Gene Research Center, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, ChinaCollege of Management Science and Engineering, Hebei University of Economics and Business, Shijiazhuang 050062, ChinaKey Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, ChinaKey Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, ChinaInstitute of Hulun Buir Forestry and Grassland Science Research, Hunlunbeier 021008, ChinaKey Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, ChinaKey Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, ChinaSheepgrass is a perennial native grass species in China, and it can tolerate high levels of salt stress with an aggressive and vigorous rhizome system. Many salt-stress-responsive genes have been identified in sheepgrass. In this study, we report the cloning and characterization of a novel salt-induced gene, <i>LcSAIN3</i> (<i>Leymus chinensis</i> salt-induced 3), from sheepgrass. Expression analysis confirmed that <i>LcSAIN3</i> was induced by PEG, ABA, and salt treatments, and the expression of <i>LcSAIN3</i> was significantly increased in salt-tolerant germplasms under salt treatment. Subcellular localization analysis indicated that the GFP-LcSAIN3 protein was mainly localized in the chloroplasts. The heterologous expression of <i>LcSAIN3</i> in Arabidopsis increased the seed germination rate of transgenic plants under salt, ABA, and mannitol treatments. The seedling survival rate, plant height, and fresh weight of the transgenic plants were higher than those of WT plants under salt stress. The overexpression of <i>LcSAIN3</i> caused a relatively high accumulation of free proline, enhanced SOD activity, and led to the upregulation of several stress-responsive genes such as <i>AtRD26</i>, <i>AtRD29B</i>, <i>AtSOS1,</i> and <i>AtP5CS1</i>. These results suggest that <i>LcSAIN3</i> could be a potential target for molecular breeding to improve plants’ salt tolerance.https://www.mdpi.com/2073-4425/12/12/1994sheepgrasschloroplastsalt stressseed germination |
| spellingShingle | Xiaoxia Li Weiguang Yang Junting Jia Pincang Zhao Dongmei Qi Shuangyan Chen Li Cheng Liqin Cheng Gongshe Liu Ectopic Expression of a Salt-Inducible Gene, <i>LcSAIN3</i>, from Sheepgrass Improves Seed Germination and Seedling Growth under Salt Stress in Arabidopsis Genes sheepgrass chloroplast salt stress seed germination |
| title | Ectopic Expression of a Salt-Inducible Gene, <i>LcSAIN3</i>, from Sheepgrass Improves Seed Germination and Seedling Growth under Salt Stress in Arabidopsis |
| title_full | Ectopic Expression of a Salt-Inducible Gene, <i>LcSAIN3</i>, from Sheepgrass Improves Seed Germination and Seedling Growth under Salt Stress in Arabidopsis |
| title_fullStr | Ectopic Expression of a Salt-Inducible Gene, <i>LcSAIN3</i>, from Sheepgrass Improves Seed Germination and Seedling Growth under Salt Stress in Arabidopsis |
| title_full_unstemmed | Ectopic Expression of a Salt-Inducible Gene, <i>LcSAIN3</i>, from Sheepgrass Improves Seed Germination and Seedling Growth under Salt Stress in Arabidopsis |
| title_short | Ectopic Expression of a Salt-Inducible Gene, <i>LcSAIN3</i>, from Sheepgrass Improves Seed Germination and Seedling Growth under Salt Stress in Arabidopsis |
| title_sort | ectopic expression of a salt inducible gene i lcsain3 i from sheepgrass improves seed germination and seedling growth under salt stress in arabidopsis |
| topic | sheepgrass chloroplast salt stress seed germination |
| url | https://www.mdpi.com/2073-4425/12/12/1994 |
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