New insights into Saccharomyces cerevisiae induced calcium carbonate precipitation
Our previous study reported that Saccharomyces cerevisiae could induce calcium carbonate (CaCO3) precipitation, but the associated mechanism was unclear. In the present study, Saccharomyces cerevisiae was cultured under various conditions, including the presence of different organic acids and initia...
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Frontiers Media S.A.
2023-08-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1261205/full |
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| author | Tianxiao Li Tianxiao Li Tianxiao Li Tianxiao Li Huabing Zhang Huabing Zhang Xiang Tan Xiang Tan Rui Zhang Rui Zhang Fasi Wu Fasi Wu Zongren Yu Zongren Yu Bomin Su Bomin Su |
| author_facet | Tianxiao Li Tianxiao Li Tianxiao Li Tianxiao Li Huabing Zhang Huabing Zhang Xiang Tan Xiang Tan Rui Zhang Rui Zhang Fasi Wu Fasi Wu Zongren Yu Zongren Yu Bomin Su Bomin Su |
| author_sort | Tianxiao Li |
| collection | DOAJ |
| description | Our previous study reported that Saccharomyces cerevisiae could induce calcium carbonate (CaCO3) precipitation, but the associated mechanism was unclear. In the present study, Saccharomyces cerevisiae was cultured under various conditions, including the presence of different organic acids and initial pH, and the yields of CaCO3 formation induced by the different organic acids were compared. The metabolism of organic acid by the metabolites of S. cerevisiae was also assessed in vitro. The SEM-EDS and XRD results showed that only acetate acid, pyruvic acid, and α-ketoglutaric acid could induce CaCO3 formation, and the weight order of the produced CaCO3 was pyruvic acid, acetate acid, α-ketoglutaric acid. In addition, the presence of only yeast metabolites and the initial neutral or alkaline environment also limited the CaCO3 formation. These results illustrated that organic acid oxidation intracellularly, especially the tricarboxylic acid cycle, was the major mechanism, and the CaCO3 yield was related to the amount of CO2 produced by the metabolism of organic acids. These findings will deepen the knowledge of the mineralization capacity of S. cerevisiae and provide a theoretical basis for the future application of yeast as an alternative microorganism in MICP. |
| first_indexed | 2024-03-12T11:49:58Z |
| format | Article |
| id | doaj.art-b46199bcad8945d9b29163f2c2e97386 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-03-12T11:49:58Z |
| publishDate | 2023-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-b46199bcad8945d9b29163f2c2e973862023-08-31T11:22:28ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852023-08-011110.3389/fbioe.2023.12612051261205New insights into Saccharomyces cerevisiae induced calcium carbonate precipitationTianxiao Li0Tianxiao Li1Tianxiao Li2Tianxiao Li3Huabing Zhang4Huabing Zhang5Xiang Tan6Xiang Tan7Rui Zhang8Rui Zhang9Fasi Wu10Fasi Wu11Zongren Yu12Zongren Yu13Bomin Su14Bomin Su15Dunhuang Academy, The Conservation Institute, Dunhuang, ChinaNational Research Center for Conservation of Ancient Wall Paintings and Earthen Sites, Dunhuang, ChinaJoint International Research Laboratory of Environmental and Social Archaeology, Shandong University, Qingdao, ChinaInstitute of Cultural Heritage, Shandong University, Qingdao, ChinaDunhuang Academy, The Conservation Institute, Dunhuang, ChinaNational Research Center for Conservation of Ancient Wall Paintings and Earthen Sites, Dunhuang, ChinaDunhuang Academy, The Conservation Institute, Dunhuang, ChinaNational Research Center for Conservation of Ancient Wall Paintings and Earthen Sites, Dunhuang, ChinaDunhuang Academy, The Conservation Institute, Dunhuang, ChinaNational Research Center for Conservation of Ancient Wall Paintings and Earthen Sites, Dunhuang, ChinaDunhuang Academy, The Conservation Institute, Dunhuang, ChinaNational Research Center for Conservation of Ancient Wall Paintings and Earthen Sites, Dunhuang, ChinaDunhuang Academy, The Conservation Institute, Dunhuang, ChinaNational Research Center for Conservation of Ancient Wall Paintings and Earthen Sites, Dunhuang, ChinaDunhuang Academy, The Conservation Institute, Dunhuang, ChinaNational Research Center for Conservation of Ancient Wall Paintings and Earthen Sites, Dunhuang, ChinaOur previous study reported that Saccharomyces cerevisiae could induce calcium carbonate (CaCO3) precipitation, but the associated mechanism was unclear. In the present study, Saccharomyces cerevisiae was cultured under various conditions, including the presence of different organic acids and initial pH, and the yields of CaCO3 formation induced by the different organic acids were compared. The metabolism of organic acid by the metabolites of S. cerevisiae was also assessed in vitro. The SEM-EDS and XRD results showed that only acetate acid, pyruvic acid, and α-ketoglutaric acid could induce CaCO3 formation, and the weight order of the produced CaCO3 was pyruvic acid, acetate acid, α-ketoglutaric acid. In addition, the presence of only yeast metabolites and the initial neutral or alkaline environment also limited the CaCO3 formation. These results illustrated that organic acid oxidation intracellularly, especially the tricarboxylic acid cycle, was the major mechanism, and the CaCO3 yield was related to the amount of CO2 produced by the metabolism of organic acids. These findings will deepen the knowledge of the mineralization capacity of S. cerevisiae and provide a theoretical basis for the future application of yeast as an alternative microorganism in MICP.https://www.frontiersin.org/articles/10.3389/fbioe.2023.1261205/fullSaccharomyces cerevisiaemicrobially induced calcium carbonate precipitationTCA cycleinitial pHorganic acids |
| spellingShingle | Tianxiao Li Tianxiao Li Tianxiao Li Tianxiao Li Huabing Zhang Huabing Zhang Xiang Tan Xiang Tan Rui Zhang Rui Zhang Fasi Wu Fasi Wu Zongren Yu Zongren Yu Bomin Su Bomin Su New insights into Saccharomyces cerevisiae induced calcium carbonate precipitation Frontiers in Bioengineering and Biotechnology Saccharomyces cerevisiae microbially induced calcium carbonate precipitation TCA cycle initial pH organic acids |
| title | New insights into Saccharomyces cerevisiae induced calcium carbonate precipitation |
| title_full | New insights into Saccharomyces cerevisiae induced calcium carbonate precipitation |
| title_fullStr | New insights into Saccharomyces cerevisiae induced calcium carbonate precipitation |
| title_full_unstemmed | New insights into Saccharomyces cerevisiae induced calcium carbonate precipitation |
| title_short | New insights into Saccharomyces cerevisiae induced calcium carbonate precipitation |
| title_sort | new insights into saccharomyces cerevisiae induced calcium carbonate precipitation |
| topic | Saccharomyces cerevisiae microbially induced calcium carbonate precipitation TCA cycle initial pH organic acids |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1261205/full |
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