Combination of curcumin and catalase protects against chondrocyte injury and knee osteoarthritis progression by suppressing oxidative stress
Knee Osteoarthritis (KOA) is an age-related progressive degenerative joint disease, which is featured with pain, joint deformity, and disability. Accumulating evidence indicated oxidative stress plays a crucial role in the occurrence and development of KOA. Curcumin is a polyphenolic compound with s...
| Main Authors: | , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-12-01
|
| Series: | Biomedicine & Pharmacotherapy |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0753332223015494 |
| _version_ | 1797630628794990592 |
|---|---|
| author | Bohao Chen Qi He Chuyi Chen Yuewei Lin Jiacong Xiao Zhaofeng Pan Miao Li Shaocong Li Junzheng Yang FanChen Wang Jiaxu Zeng Yanzi Yi Weijin Chi Kai Meng Haibin Wang Peng Chen |
| author_facet | Bohao Chen Qi He Chuyi Chen Yuewei Lin Jiacong Xiao Zhaofeng Pan Miao Li Shaocong Li Junzheng Yang FanChen Wang Jiaxu Zeng Yanzi Yi Weijin Chi Kai Meng Haibin Wang Peng Chen |
| author_sort | Bohao Chen |
| collection | DOAJ |
| description | Knee Osteoarthritis (KOA) is an age-related progressive degenerative joint disease, which is featured with pain, joint deformity, and disability. Accumulating evidence indicated oxidative stress plays a crucial role in the occurrence and development of KOA. Curcumin is a polyphenolic compound with significant antioxidant activity among various diseases while catalase (CAT) is an enzyme degrading hydrogen peroxide in treating oxidative diseases. We previously showed that the expression of CAT was low in cartilage. However, the combination of curcumin and CAT in KOA is still elusive. In this study, we demonstrated that the combination of curcumin and CAT has the potential to inhibit the IL1β-induced chondrocyte apoptosis without cytotoxicity in vitro. Mechanistically, we found that the synergistic application curcumin and CAT not only promotes curcumin's regulation of the NRF2/HO-1 signaling pathway to enhance antioxidant enzyme expression to remove superoxide radicals, but also CAT can further remove downstream hydrogen peroxide which enhances the ability to scavenge reactive oxygen species (ROS). In vivo, studies revealed that combination of curcumin and catalase could better inhibit oxidative stress-induced chondrocyte injury by promoting the expression of ROS scavenging enzymes. In sum, the combination of curcumin and catalase can be used to treat KOA. Thus, combination of curcumin and catalase may act as a novel therapeutic agent to manage KOA and our research gives a rationale for their combined use in the therapeutic of KOA. |
| first_indexed | 2024-03-11T11:10:52Z |
| format | Article |
| id | doaj.art-b46305acedd2446caa602ab631472003 |
| institution | Directory Open Access Journal |
| issn | 0753-3322 |
| language | English |
| last_indexed | 2024-03-11T11:10:52Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biomedicine & Pharmacotherapy |
| spelling | doaj.art-b46305acedd2446caa602ab6314720032023-11-12T04:39:11ZengElsevierBiomedicine & Pharmacotherapy0753-33222023-12-01168115751Combination of curcumin and catalase protects against chondrocyte injury and knee osteoarthritis progression by suppressing oxidative stressBohao Chen0Qi He1Chuyi Chen2Yuewei Lin3Jiacong Xiao4Zhaofeng Pan5Miao Li6Shaocong Li7Junzheng Yang8FanChen Wang9Jiaxu Zeng10Yanzi Yi11Weijin Chi12Kai Meng13Haibin Wang14Peng Chen151st School of Medicine, Guangzhou University of Chinese Medicine, 12 Jichang Road, Baiyun Area, Guangzhou 510405, PR China; The Laboratory of Orthopaedics and Traumatology of Lingnan Medical Research Center, Guangzhou University of Chinese Medicine, Guangzhou 510405, PR China1st School of Medicine, Guangzhou University of Chinese Medicine, 12 Jichang Road, Baiyun Area, Guangzhou 510405, PR China; The Laboratory of Orthopaedics and Traumatology of Lingnan Medical Research Center, Guangzhou University of Chinese Medicine, Guangzhou 510405, PR China; Department of Orthopaedic Surgery, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA1st School of Medicine, Guangzhou University of Chinese Medicine, 12 Jichang Road, Baiyun Area, Guangzhou 510405, PR China; The Laboratory of Orthopaedics and Traumatology of Lingnan Medical Research Center, Guangzhou University of Chinese Medicine, Guangzhou 510405, PR China1st School of Medicine, Guangzhou University of Chinese Medicine, 12 Jichang Road, Baiyun Area, Guangzhou 510405, PR China; The Laboratory of Orthopaedics and Traumatology of Lingnan Medical Research Center, Guangzhou University of Chinese Medicine, Guangzhou 510405, PR China1st School of Medicine, Guangzhou University of Chinese Medicine, 12 Jichang Road, Baiyun Area, Guangzhou 510405, PR China; The Laboratory of Orthopaedics and Traumatology of Lingnan Medical Research Center, Guangzhou University of Chinese Medicine, Guangzhou 510405, PR China1st School of Medicine, Guangzhou University of Chinese Medicine, 12 Jichang Road, Baiyun Area, Guangzhou 510405, PR China; The Laboratory of Orthopaedics and Traumatology of Lingnan Medical Research Center, Guangzhou University of Chinese Medicine, Guangzhou 510405, PR China1st School of Medicine, Guangzhou University of Chinese Medicine, 12 Jichang Road, Baiyun Area, Guangzhou 510405, PR China; The Laboratory of Orthopaedics and Traumatology of Lingnan Medical Research Center, Guangzhou University of Chinese Medicine, Guangzhou 510405, PR China1st School of Medicine, Guangzhou University of Chinese Medicine, 12 Jichang Road, Baiyun Area, Guangzhou 510405, PR China; The Laboratory of Orthopaedics and Traumatology of Lingnan Medical Research Center, Guangzhou University of Chinese Medicine, Guangzhou 510405, PR China1st School of Medicine, Guangzhou University of Chinese Medicine, 12 Jichang Road, Baiyun Area, Guangzhou 510405, PR China; The Laboratory of Orthopaedics and Traumatology of Lingnan Medical Research Center, Guangzhou University of Chinese Medicine, Guangzhou 510405, PR China1st School of Medicine, Guangzhou University of Chinese Medicine, 12 Jichang Road, Baiyun Area, Guangzhou 510405, PR China; The Laboratory of Orthopaedics and Traumatology of Lingnan Medical Research Center, Guangzhou University of Chinese Medicine, Guangzhou 510405, PR China1st School of Medicine, Guangzhou University of Chinese Medicine, 12 Jichang Road, Baiyun Area, Guangzhou 510405, PR China; The Laboratory of Orthopaedics and Traumatology of Lingnan Medical Research Center, Guangzhou University of Chinese Medicine, Guangzhou 510405, PR China1st School of Medicine, Guangzhou University of Chinese Medicine, 12 Jichang Road, Baiyun Area, Guangzhou 510405, PR China; The Third Affiliated Medical College of Guangzhou University of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510405, PR China1st School of Medicine, Guangzhou University of Chinese Medicine, 12 Jichang Road, Baiyun Area, Guangzhou 510405, PR China; The Laboratory of Orthopaedics and Traumatology of Lingnan Medical Research Center, Guangzhou University of Chinese Medicine, Guangzhou 510405, PR ChinaDepartment of Orthopaedics Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250014, PR China; Correspondence to: 12 Jichang Road, Baiyun Area, Guangzhou, PR China.Department of Orthopaedics, First Affiliated Hospital, Guangzhou University of Chinese Medicine, 12 Jichang Road, Baiyun Area, Guangzhou 510405, PR China; Correspondence to: 12 Jichang Road, Baiyun Area, Guangzhou, PR China.Department of Orthopaedics, First Affiliated Hospital, Guangzhou University of Chinese Medicine, 12 Jichang Road, Baiyun Area, Guangzhou 510405, PR China; Correspondence to: 12 Jichang Road, Baiyun Area, Guangzhou, PR China.Knee Osteoarthritis (KOA) is an age-related progressive degenerative joint disease, which is featured with pain, joint deformity, and disability. Accumulating evidence indicated oxidative stress plays a crucial role in the occurrence and development of KOA. Curcumin is a polyphenolic compound with significant antioxidant activity among various diseases while catalase (CAT) is an enzyme degrading hydrogen peroxide in treating oxidative diseases. We previously showed that the expression of CAT was low in cartilage. However, the combination of curcumin and CAT in KOA is still elusive. In this study, we demonstrated that the combination of curcumin and CAT has the potential to inhibit the IL1β-induced chondrocyte apoptosis without cytotoxicity in vitro. Mechanistically, we found that the synergistic application curcumin and CAT not only promotes curcumin's regulation of the NRF2/HO-1 signaling pathway to enhance antioxidant enzyme expression to remove superoxide radicals, but also CAT can further remove downstream hydrogen peroxide which enhances the ability to scavenge reactive oxygen species (ROS). In vivo, studies revealed that combination of curcumin and catalase could better inhibit oxidative stress-induced chondrocyte injury by promoting the expression of ROS scavenging enzymes. In sum, the combination of curcumin and catalase can be used to treat KOA. Thus, combination of curcumin and catalase may act as a novel therapeutic agent to manage KOA and our research gives a rationale for their combined use in the therapeutic of KOA.http://www.sciencedirect.com/science/article/pii/S0753332223015494CurcuminCatalaseReactive oxygen speciesNrf2/HO-1 signaling pathway |
| spellingShingle | Bohao Chen Qi He Chuyi Chen Yuewei Lin Jiacong Xiao Zhaofeng Pan Miao Li Shaocong Li Junzheng Yang FanChen Wang Jiaxu Zeng Yanzi Yi Weijin Chi Kai Meng Haibin Wang Peng Chen Combination of curcumin and catalase protects against chondrocyte injury and knee osteoarthritis progression by suppressing oxidative stress Biomedicine & Pharmacotherapy Curcumin Catalase Reactive oxygen species Nrf2/HO-1 signaling pathway |
| title | Combination of curcumin and catalase protects against chondrocyte injury and knee osteoarthritis progression by suppressing oxidative stress |
| title_full | Combination of curcumin and catalase protects against chondrocyte injury and knee osteoarthritis progression by suppressing oxidative stress |
| title_fullStr | Combination of curcumin and catalase protects against chondrocyte injury and knee osteoarthritis progression by suppressing oxidative stress |
| title_full_unstemmed | Combination of curcumin and catalase protects against chondrocyte injury and knee osteoarthritis progression by suppressing oxidative stress |
| title_short | Combination of curcumin and catalase protects against chondrocyte injury and knee osteoarthritis progression by suppressing oxidative stress |
| title_sort | combination of curcumin and catalase protects against chondrocyte injury and knee osteoarthritis progression by suppressing oxidative stress |
| topic | Curcumin Catalase Reactive oxygen species Nrf2/HO-1 signaling pathway |
| url | http://www.sciencedirect.com/science/article/pii/S0753332223015494 |
| work_keys_str_mv | AT bohaochen combinationofcurcuminandcatalaseprotectsagainstchondrocyteinjuryandkneeosteoarthritisprogressionbysuppressingoxidativestress AT qihe combinationofcurcuminandcatalaseprotectsagainstchondrocyteinjuryandkneeosteoarthritisprogressionbysuppressingoxidativestress AT chuyichen combinationofcurcuminandcatalaseprotectsagainstchondrocyteinjuryandkneeosteoarthritisprogressionbysuppressingoxidativestress AT yueweilin combinationofcurcuminandcatalaseprotectsagainstchondrocyteinjuryandkneeosteoarthritisprogressionbysuppressingoxidativestress AT jiacongxiao combinationofcurcuminandcatalaseprotectsagainstchondrocyteinjuryandkneeosteoarthritisprogressionbysuppressingoxidativestress AT zhaofengpan combinationofcurcuminandcatalaseprotectsagainstchondrocyteinjuryandkneeosteoarthritisprogressionbysuppressingoxidativestress AT miaoli combinationofcurcuminandcatalaseprotectsagainstchondrocyteinjuryandkneeosteoarthritisprogressionbysuppressingoxidativestress AT shaocongli combinationofcurcuminandcatalaseprotectsagainstchondrocyteinjuryandkneeosteoarthritisprogressionbysuppressingoxidativestress AT junzhengyang combinationofcurcuminandcatalaseprotectsagainstchondrocyteinjuryandkneeosteoarthritisprogressionbysuppressingoxidativestress AT fanchenwang combinationofcurcuminandcatalaseprotectsagainstchondrocyteinjuryandkneeosteoarthritisprogressionbysuppressingoxidativestress AT jiaxuzeng combinationofcurcuminandcatalaseprotectsagainstchondrocyteinjuryandkneeosteoarthritisprogressionbysuppressingoxidativestress AT yanziyi combinationofcurcuminandcatalaseprotectsagainstchondrocyteinjuryandkneeosteoarthritisprogressionbysuppressingoxidativestress AT weijinchi combinationofcurcuminandcatalaseprotectsagainstchondrocyteinjuryandkneeosteoarthritisprogressionbysuppressingoxidativestress AT kaimeng combinationofcurcuminandcatalaseprotectsagainstchondrocyteinjuryandkneeosteoarthritisprogressionbysuppressingoxidativestress AT haibinwang combinationofcurcuminandcatalaseprotectsagainstchondrocyteinjuryandkneeosteoarthritisprogressionbysuppressingoxidativestress AT pengchen combinationofcurcuminandcatalaseprotectsagainstchondrocyteinjuryandkneeosteoarthritisprogressionbysuppressingoxidativestress |