Whole-genome sequence of a high-temperature edible mushroom Pleurotus giganteus (zhudugu)
Most of the sequenced wood-rotting edible mushroom produce fruiting body at relatively low temperatures. Little information has been known about the high-temperature wood-rotting mushroom. Here, we performed de novo sequencing and assembly of the genome of a high-temperature edible mushroom Pleurotu...
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Frontiers Media S.A.
2022-08-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.941889/full |
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| author | Hailong Yu Meiyan Zhang Yating Sun Yating Sun Qiaozhen Li Jianyu Liu Chunyan Song Xiaodong Shang Qi Tan Lujun Zhang Hao Yu |
| author_facet | Hailong Yu Meiyan Zhang Yating Sun Yating Sun Qiaozhen Li Jianyu Liu Chunyan Song Xiaodong Shang Qi Tan Lujun Zhang Hao Yu |
| author_sort | Hailong Yu |
| collection | DOAJ |
| description | Most of the sequenced wood-rotting edible mushroom produce fruiting body at relatively low temperatures. Little information has been known about the high-temperature wood-rotting mushroom. Here, we performed de novo sequencing and assembly of the genome of a high-temperature edible mushroom Pleurotus giganteus from a monokaryotic strain zhudugu2 using the Illumina and Pac-Bio CLR sequencing technologies. P. giganteus, also known as Zhudugu in China, is a well-known culinary edible mushroom that has been widely distributed and cultivated in China, Southeast Asia, and South Asia. The genome consists of 40.00 Mb in 27 contigs with a contig N50 of 4.384 Mb. Phylogenetic analysis reveals that P. giganteus and other strains in Pleurotus clustered in one clade. Phylogenetic analysis and average nucleotide identity analysis indicated that the P. giganteus genome showed a closer relationship with other Pleurotus species. Chromosome collinearity analysis revealed a high level of collinearity between P. ostreatus and P. giganteus. There are 12,628 protein-coding genes annotated in this monoploid genome. A total of 481 enzymes accounting for 514 carbohydrate-active enzymes (CAZymes) terms were identified in the P. giganteus genome, including 15 laccases and 10 class II peroxidases predicted in the genome, which revealed the robustness of lignocellulose degradation capacity of P. giganteus. The mating-A type locus of P. giganteus consisted of a pair of homeodomain mating-type genes HD1 and HD2. The mating-B type locus of P. giganteus consisted of at least four pheromone receptor genes and three pheromone genes. The genome is not only beneficial for the genome-assisted breeding of this mushroom but also helps us to understand the high-temperature tolerance of the edible mushroom. |
| first_indexed | 2024-12-10T15:43:15Z |
| format | Article |
| id | doaj.art-b463a8fbbde3449c8f6146003892abff |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-10T15:43:15Z |
| publishDate | 2022-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-b463a8fbbde3449c8f6146003892abff2022-12-22T01:43:02ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-08-011310.3389/fmicb.2022.941889941889Whole-genome sequence of a high-temperature edible mushroom Pleurotus giganteus (zhudugu)Hailong Yu0Meiyan Zhang1Yating Sun2Yating Sun3Qiaozhen Li4Jianyu Liu5Chunyan Song6Xiaodong Shang7Qi Tan8Lujun Zhang9Hao Yu10National Engineering Research Center of Edible Fungi, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai, ChinaNational Engineering Research Center of Edible Fungi, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai, ChinaNational Engineering Research Center of Edible Fungi, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai, ChinaCollege of Horticulture, Shenyang Agricultural University, Shenyang, ChinaNational Engineering Research Center of Edible Fungi, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai, ChinaNational Engineering Research Center of Edible Fungi, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai, ChinaNational Engineering Research Center of Edible Fungi, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai, ChinaNational Engineering Research Center of Edible Fungi, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai, ChinaNational Engineering Research Center of Edible Fungi, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai, ChinaNational Engineering Research Center of Edible Fungi, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai, ChinaShandong Provincial Key Laboratory of Applied Mycology, School of Life Sciences, Qingdao Agricultural University, Qingdao, ChinaMost of the sequenced wood-rotting edible mushroom produce fruiting body at relatively low temperatures. Little information has been known about the high-temperature wood-rotting mushroom. Here, we performed de novo sequencing and assembly of the genome of a high-temperature edible mushroom Pleurotus giganteus from a monokaryotic strain zhudugu2 using the Illumina and Pac-Bio CLR sequencing technologies. P. giganteus, also known as Zhudugu in China, is a well-known culinary edible mushroom that has been widely distributed and cultivated in China, Southeast Asia, and South Asia. The genome consists of 40.00 Mb in 27 contigs with a contig N50 of 4.384 Mb. Phylogenetic analysis reveals that P. giganteus and other strains in Pleurotus clustered in one clade. Phylogenetic analysis and average nucleotide identity analysis indicated that the P. giganteus genome showed a closer relationship with other Pleurotus species. Chromosome collinearity analysis revealed a high level of collinearity between P. ostreatus and P. giganteus. There are 12,628 protein-coding genes annotated in this monoploid genome. A total of 481 enzymes accounting for 514 carbohydrate-active enzymes (CAZymes) terms were identified in the P. giganteus genome, including 15 laccases and 10 class II peroxidases predicted in the genome, which revealed the robustness of lignocellulose degradation capacity of P. giganteus. The mating-A type locus of P. giganteus consisted of a pair of homeodomain mating-type genes HD1 and HD2. The mating-B type locus of P. giganteus consisted of at least four pheromone receptor genes and three pheromone genes. The genome is not only beneficial for the genome-assisted breeding of this mushroom but also helps us to understand the high-temperature tolerance of the edible mushroom.https://www.frontiersin.org/articles/10.3389/fmicb.2022.941889/fullPleurotus giganteusgenomeedible mushroomwhite-rot fungimating locusCAZymes |
| spellingShingle | Hailong Yu Meiyan Zhang Yating Sun Yating Sun Qiaozhen Li Jianyu Liu Chunyan Song Xiaodong Shang Qi Tan Lujun Zhang Hao Yu Whole-genome sequence of a high-temperature edible mushroom Pleurotus giganteus (zhudugu) Frontiers in Microbiology Pleurotus giganteus genome edible mushroom white-rot fungi mating locus CAZymes |
| title | Whole-genome sequence of a high-temperature edible mushroom Pleurotus giganteus (zhudugu) |
| title_full | Whole-genome sequence of a high-temperature edible mushroom Pleurotus giganteus (zhudugu) |
| title_fullStr | Whole-genome sequence of a high-temperature edible mushroom Pleurotus giganteus (zhudugu) |
| title_full_unstemmed | Whole-genome sequence of a high-temperature edible mushroom Pleurotus giganteus (zhudugu) |
| title_short | Whole-genome sequence of a high-temperature edible mushroom Pleurotus giganteus (zhudugu) |
| title_sort | whole genome sequence of a high temperature edible mushroom pleurotus giganteus zhudugu |
| topic | Pleurotus giganteus genome edible mushroom white-rot fungi mating locus CAZymes |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.941889/full |
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