Interleukin-22-induced β‑defensin-2 expression by intranasal immunization with Streptococcus pneumoniae RrgB epitopes
Background Streptococcus pneumoniae causes pneumococcal disease, which is responsible for millions of deaths worldwide. Various pneumococcal vaccine candidates have been developed to prevent S. pneumoniae infection, one of which is an epitope-based vaccine. This study aimed to prove that intranasal...
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| Format: | Article |
| Language: | English |
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Faculty of Medicine Trisakti University
2022-03-01
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| Series: | Universa Medicina |
| Subjects: | |
| Online Access: | https://univmed.org/ejurnal/index.php/medicina/article/view/1237 |
| _version_ | 1819061041488723968 |
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| author | Diana Chusna Mufida Antonius Dwi Saputra Bagus Hermansyah Dini Agustina Muhammad Ali Shodikin Yunita Armiyanti |
| author_facet | Diana Chusna Mufida Antonius Dwi Saputra Bagus Hermansyah Dini Agustina Muhammad Ali Shodikin Yunita Armiyanti |
| author_sort | Diana Chusna Mufida |
| collection | DOAJ |
| description |
Background
Streptococcus pneumoniae causes pneumococcal disease, which is responsible for millions of deaths worldwide. Various pneumococcal vaccine candidates have been developed to prevent S. pneumoniae infection, one of which is an epitope-based vaccine. This study aimed to prove that intranasal immunization with each of the five S. pneumoniae RrgB epitopes can induce a mucosal immune response by increasing the β-defensin-2 concentration through upregulation of interleukin (IL)-22 expression.
Methods
An experimental laboratory study was conducted using 28 male Wistar rats aged 3-4 months, that were randomly divided into 7 groups containing four rats each. Group 1 was given 40 mL of phosphate-buffered saline (PBS) only (control group). Group 2 was the adjuvant group that received 40 mL PBS containing 2 ìg cholera toxin B (CTB), and groups 3-7 were immunized with 40 mL PBS containing a combination of adjuvant and one of the five different S. pneumoniae RrgB epitopes. The concentrations of IL-22 and β-defensin-2 from nasal rinse examination were measured by means of ELISA. The Kruskal-Wallis test, followed by the Mann-Whitney post-hoc test were used for statistical analysis.
Results
Rats immunized with the adjuvant-epitope combination had significantly higher β-defensin-2 and IL-22 levels than the control group (p=0.030; p=0.018, respectively), according to the Kruskal-Wallis test. And the Mann-Whitney statistical test, showed there was a significant increase in β-defensin-2 and IL-22 levels.
Conclusions
Intranasal immunization with epitope 1 of the S. pneumoniae RrgB can increase β-defensin-2 expression significantly and has a greater potential to be developed into a pneumococcal vaccine.
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| first_indexed | 2024-12-21T14:36:35Z |
| format | Article |
| id | doaj.art-b4681a82e2684dc6810f6031d437db6b |
| institution | Directory Open Access Journal |
| issn | 1907-3062 2407-2230 |
| language | English |
| last_indexed | 2024-12-21T14:36:35Z |
| publishDate | 2022-03-01 |
| publisher | Faculty of Medicine Trisakti University |
| record_format | Article |
| series | Universa Medicina |
| spelling | doaj.art-b4681a82e2684dc6810f6031d437db6b2022-12-21T19:00:18ZengFaculty of Medicine Trisakti UniversityUniversa Medicina1907-30622407-22302022-03-0141110.18051/UnivMed.2022.v41.37-46Interleukin-22-induced β‑defensin-2 expression by intranasal immunization with Streptococcus pneumoniae RrgB epitopesDiana Chusna Mufida0Antonius Dwi Saputra1Bagus Hermansyah2Dini Agustina3Muhammad Ali Shodikin4Yunita Armiyanti5Department of Microbiology, Faculty of Medicine, University of Jember, JemberUndergraduate Program, Faculty of Medicine, University of Jember, JemberDepartment of Parasitology, Faculty of Medicine, University of Jember, JemberDepartment of Microbiology, Faculty of Medicine, University of Jember, JemberDepartment of Microbiology, Faculty of Medicine, University of Jember, JemberDepartment of Parasitology, Faculty of Medicine, University of Jember, Jember Background Streptococcus pneumoniae causes pneumococcal disease, which is responsible for millions of deaths worldwide. Various pneumococcal vaccine candidates have been developed to prevent S. pneumoniae infection, one of which is an epitope-based vaccine. This study aimed to prove that intranasal immunization with each of the five S. pneumoniae RrgB epitopes can induce a mucosal immune response by increasing the β-defensin-2 concentration through upregulation of interleukin (IL)-22 expression. Methods An experimental laboratory study was conducted using 28 male Wistar rats aged 3-4 months, that were randomly divided into 7 groups containing four rats each. Group 1 was given 40 mL of phosphate-buffered saline (PBS) only (control group). Group 2 was the adjuvant group that received 40 mL PBS containing 2 ìg cholera toxin B (CTB), and groups 3-7 were immunized with 40 mL PBS containing a combination of adjuvant and one of the five different S. pneumoniae RrgB epitopes. The concentrations of IL-22 and β-defensin-2 from nasal rinse examination were measured by means of ELISA. The Kruskal-Wallis test, followed by the Mann-Whitney post-hoc test were used for statistical analysis. Results Rats immunized with the adjuvant-epitope combination had significantly higher β-defensin-2 and IL-22 levels than the control group (p=0.030; p=0.018, respectively), according to the Kruskal-Wallis test. And the Mann-Whitney statistical test, showed there was a significant increase in β-defensin-2 and IL-22 levels. Conclusions Intranasal immunization with epitope 1 of the S. pneumoniae RrgB can increase β-defensin-2 expression significantly and has a greater potential to be developed into a pneumococcal vaccine. https://univmed.org/ejurnal/index.php/medicina/article/view/1237Intranasal ImmunizationEpitopes-based vaccineProtein RrgB Streptococcus pneumoniaeβ‑defensin-2 |
| spellingShingle | Diana Chusna Mufida Antonius Dwi Saputra Bagus Hermansyah Dini Agustina Muhammad Ali Shodikin Yunita Armiyanti Interleukin-22-induced β‑defensin-2 expression by intranasal immunization with Streptococcus pneumoniae RrgB epitopes Universa Medicina Intranasal Immunization Epitopes-based vaccine Protein RrgB Streptococcus pneumoniae β‑defensin-2 |
| title | Interleukin-22-induced β‑defensin-2 expression by intranasal immunization with Streptococcus pneumoniae RrgB epitopes |
| title_full | Interleukin-22-induced β‑defensin-2 expression by intranasal immunization with Streptococcus pneumoniae RrgB epitopes |
| title_fullStr | Interleukin-22-induced β‑defensin-2 expression by intranasal immunization with Streptococcus pneumoniae RrgB epitopes |
| title_full_unstemmed | Interleukin-22-induced β‑defensin-2 expression by intranasal immunization with Streptococcus pneumoniae RrgB epitopes |
| title_short | Interleukin-22-induced β‑defensin-2 expression by intranasal immunization with Streptococcus pneumoniae RrgB epitopes |
| title_sort | interleukin 22 induced β defensin 2 expression by intranasal immunization with streptococcus pneumoniae rrgb epitopes |
| topic | Intranasal Immunization Epitopes-based vaccine Protein RrgB Streptococcus pneumoniae β‑defensin-2 |
| url | https://univmed.org/ejurnal/index.php/medicina/article/view/1237 |
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