Sequence feature and expression profile of a tumor suppressor QM protein gene from hard clam Meretrix meretrix
The present study describes the molecular characterization and transcriptional features of a tumor suppressor QM protein gene, MmQM, in Meretrix meretrix. The full-length cDNA (819 bp) of MmQM consists of a 657 bp opening reading frame (ORF) encoding a 218 amino acid protein with a calculated molecu...
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| Format: | Article |
| Language: | English |
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University of Modena and Reggio Emilia
2019-10-01
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| Series: | Invertebrate Survival Journal |
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| Online Access: | https://isj02.unimore.it/index.php/ISJ/article/view/548 |
| _version_ | 1818384624012754944 |
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| author | X Y Cui J W Cheng Y Wang B Zhou |
| author_facet | X Y Cui J W Cheng Y Wang B Zhou |
| author_sort | X Y Cui |
| collection | DOAJ |
| description | The present study describes the molecular characterization and transcriptional features of a tumor suppressor QM protein gene, MmQM, in Meretrix meretrix. The full-length cDNA (819 bp) of MmQM consists of a 657 bp opening reading frame (ORF) encoding a 218 amino acid protein with a calculated molecular mass of 25.3 kDa and theoretical isoelectric point of 10.2. A ribosomal protein L10 signature, an SH3-binding motif, an antibiotic binding site, an amidation site and two protein kinase C phosphorylation sites were revealed from the MmQM sequence. Phylogenetic analysis showed that MmQM is clustered with previously identified mollusk QM proteins. MmQM mRNA transcripts were detectable in all of the examined tissues in a constitutive manner, and were significantly different from each other. After bacterial stimulation, the mRNA transcripts of MmQM in the hepatopancreas significantly increased. Hence, we conclude that MmQM could respond to pathogenic infections and it might play an important role in the innate immunity against microorganisms in the clam M. meretrix. |
| first_indexed | 2024-12-14T03:25:13Z |
| format | Article |
| id | doaj.art-b468691edc71426b8679fa514dc3ee51 |
| institution | Directory Open Access Journal |
| issn | 1824-307X |
| language | English |
| last_indexed | 2024-12-14T03:25:13Z |
| publishDate | 2019-10-01 |
| publisher | University of Modena and Reggio Emilia |
| record_format | Article |
| series | Invertebrate Survival Journal |
| spelling | doaj.art-b468691edc71426b8679fa514dc3ee512022-12-21T23:18:55ZengUniversity of Modena and Reggio EmiliaInvertebrate Survival Journal1824-307X2019-10-01Sequence feature and expression profile of a tumor suppressor QM protein gene from hard clam Meretrix meretrixX Y Cui0J W Cheng1Y Wang2B Zhou3Department of Marine Ecology, College of Marine Life Science, Ocean University of China, Qingdao 266003, ChinaDepartment of Marine Ecology, College of Marine Life Science, Ocean University of China, Qingdao 266003, ChinaDepartment of Marine Ecology, College of Marine Life Science, Ocean University of China, Qingdao 266003, China ; Laboratory for Marine Ecology and Environmental Science, Pilot National Laboratory for Marine Science and Technology, Qingdao 266237, ChinaDepartment of Marine Ecology, College of Marine Life Science, Ocean University of China, Qingdao 266003, China ; Laboratory for Marine Ecology and Environmental Science, Pilot National Laboratory for Marine Science and Technology, Qingdao 266237, ChinaThe present study describes the molecular characterization and transcriptional features of a tumor suppressor QM protein gene, MmQM, in Meretrix meretrix. The full-length cDNA (819 bp) of MmQM consists of a 657 bp opening reading frame (ORF) encoding a 218 amino acid protein with a calculated molecular mass of 25.3 kDa and theoretical isoelectric point of 10.2. A ribosomal protein L10 signature, an SH3-binding motif, an antibiotic binding site, an amidation site and two protein kinase C phosphorylation sites were revealed from the MmQM sequence. Phylogenetic analysis showed that MmQM is clustered with previously identified mollusk QM proteins. MmQM mRNA transcripts were detectable in all of the examined tissues in a constitutive manner, and were significantly different from each other. After bacterial stimulation, the mRNA transcripts of MmQM in the hepatopancreas significantly increased. Hence, we conclude that MmQM could respond to pathogenic infections and it might play an important role in the innate immunity against microorganisms in the clam M. meretrix.https://isj02.unimore.it/index.php/ISJ/article/view/548Innate immunityMeretrix meretrixQM proteinVibrio splendidus |
| spellingShingle | X Y Cui J W Cheng Y Wang B Zhou Sequence feature and expression profile of a tumor suppressor QM protein gene from hard clam Meretrix meretrix Invertebrate Survival Journal Innate immunity Meretrix meretrix QM protein Vibrio splendidus |
| title | Sequence feature and expression profile of a tumor suppressor QM protein gene from hard clam Meretrix meretrix |
| title_full | Sequence feature and expression profile of a tumor suppressor QM protein gene from hard clam Meretrix meretrix |
| title_fullStr | Sequence feature and expression profile of a tumor suppressor QM protein gene from hard clam Meretrix meretrix |
| title_full_unstemmed | Sequence feature and expression profile of a tumor suppressor QM protein gene from hard clam Meretrix meretrix |
| title_short | Sequence feature and expression profile of a tumor suppressor QM protein gene from hard clam Meretrix meretrix |
| title_sort | sequence feature and expression profile of a tumor suppressor qm protein gene from hard clam meretrix meretrix |
| topic | Innate immunity Meretrix meretrix QM protein Vibrio splendidus |
| url | https://isj02.unimore.it/index.php/ISJ/article/view/548 |
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