Angling for Uniqueness in Enzymatic Preparation of Glycosides
In the early days of biocatalysis, limitations of an enzyme modeled the enzymatic applications; nowadays the enzyme can be engineered to be suitable for the process requirements. This is a general bird’s-eye view and as such cannot be specific for articulated situations found in different classes of...
| Main Author: | |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2013-06-01
|
| Series: | Biomolecules |
| Subjects: | |
| Online Access: | http://www.mdpi.com/2218-273X/3/2/334 |
| _version_ | 1818766447555379200 |
|---|---|
| author | Antonio Trincone |
| author_facet | Antonio Trincone |
| author_sort | Antonio Trincone |
| collection | DOAJ |
| description | In the early days of biocatalysis, limitations of an enzyme modeled the enzymatic applications; nowadays the enzyme can be engineered to be suitable for the process requirements. This is a general bird’s-eye view and as such cannot be specific for articulated situations found in different classes of enzymes or for selected enzymatic processes. As far as the enzymatic preparation of glycosides is concerned, recent scientific literature is awash with examples of uniqueness related to the features of the biocatalyst (yield, substrate specificity, regioselectivity, and resistance to a particular reaction condition). The invention of glycosynthases is just one of the aspects that has thrust forward the research in this field. Protein engineering, metagenomics and reaction engineering have led to the discovery of an expanding number of novel enzymes and to the setting up of new bio-based processes for the preparation of glycosides. In this review, new examples from the last decade are compiled with attention both to cases in which naturally present, as well as genetically inserted, characteristics of the catalysts make them attractive for biocatalysis. |
| first_indexed | 2024-12-18T08:34:08Z |
| format | Article |
| id | doaj.art-b468a36c231944b997a3526c7773ac3f |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-12-18T08:34:08Z |
| publishDate | 2013-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-b468a36c231944b997a3526c7773ac3f2022-12-21T21:14:22ZengMDPI AGBiomolecules2218-273X2013-06-013233435010.3390/biom3020334Angling for Uniqueness in Enzymatic Preparation of GlycosidesAntonio TrinconeIn the early days of biocatalysis, limitations of an enzyme modeled the enzymatic applications; nowadays the enzyme can be engineered to be suitable for the process requirements. This is a general bird’s-eye view and as such cannot be specific for articulated situations found in different classes of enzymes or for selected enzymatic processes. As far as the enzymatic preparation of glycosides is concerned, recent scientific literature is awash with examples of uniqueness related to the features of the biocatalyst (yield, substrate specificity, regioselectivity, and resistance to a particular reaction condition). The invention of glycosynthases is just one of the aspects that has thrust forward the research in this field. Protein engineering, metagenomics and reaction engineering have led to the discovery of an expanding number of novel enzymes and to the setting up of new bio-based processes for the preparation of glycosides. In this review, new examples from the last decade are compiled with attention both to cases in which naturally present, as well as genetically inserted, characteristics of the catalysts make them attractive for biocatalysis.http://www.mdpi.com/2218-273X/3/2/334biocatalysisglycosidesenzymesglycosidasesglycosynthases |
| spellingShingle | Antonio Trincone Angling for Uniqueness in Enzymatic Preparation of Glycosides Biomolecules biocatalysis glycosides enzymes glycosidases glycosynthases |
| title | Angling for Uniqueness in Enzymatic Preparation of Glycosides |
| title_full | Angling for Uniqueness in Enzymatic Preparation of Glycosides |
| title_fullStr | Angling for Uniqueness in Enzymatic Preparation of Glycosides |
| title_full_unstemmed | Angling for Uniqueness in Enzymatic Preparation of Glycosides |
| title_short | Angling for Uniqueness in Enzymatic Preparation of Glycosides |
| title_sort | angling for uniqueness in enzymatic preparation of glycosides |
| topic | biocatalysis glycosides enzymes glycosidases glycosynthases |
| url | http://www.mdpi.com/2218-273X/3/2/334 |
| work_keys_str_mv | AT antoniotrincone anglingforuniquenessinenzymaticpreparationofglycosides |