Interferon Gamma Release Assays (IGRA) in the Diagnosis of Active Pulmonary Tuberculosis
Background: Tuberculosis is one of the deadliest diseases over the world. Diagnosis of pulmonary TB depends on combination various parameters. IGRA measure T cell release of IFN-γ in response to M.TB antigen. WHO policy statement as on IGRA use in low and middle income countries is not established...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Alexandria University
2014-06-01
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| Series: | Journal of High Institute of Public Health |
| Subjects: |
| _version_ | 1828899163740307456 |
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| author | Mona K. EL-Deeb Hatem A. EL Malwany Yehia M. Khalil Saher T. Mourad |
| author_facet | Mona K. EL-Deeb Hatem A. EL Malwany Yehia M. Khalil Saher T. Mourad |
| author_sort | Mona K. EL-Deeb |
| collection | DOAJ |
| description | Background: Tuberculosis is one of the deadliest diseases over the world. Diagnosis of pulmonary TB
depends on combination various parameters. IGRA measure T cell release of IFN-γ in response to M.TB
antigen. WHO policy statement as on IGRA use in low and middle income countries is not established
yet. Objective: The present study aimed to compare between tuberculin skin test (TST) and interferon
gamma release assay (IGRA) in the diagnosis of active tuberculosis infection and study the effect of 3
months of first line anti-TB therapy on the positivity of the IGRA test.
Methods: 40 Egyptian patients were included in the study, and assigned as two groups; Group I
comprised 20 patients with negative sputum for AFB by Ziehl-Neelsen stains with positive sputum
culture for M.TB and Group II included 20 patients with positive sputum and Ziehl-Neelsen for AFB
before and after 3 months of first line of anti-TB therapy. All patients were subjected to full history
taking, clinical examination, X- ray chest, lab investigations, ESR measurements, microbiological tests
and ELISA measurement of Quantiferon-TB Gold.
Results: Lower significant values were found in group II after treatment than before treatment regarding
clinical parameters and 1st and 2nd hours ESR. IGRA test and TST showed sensitivity (91.18%, 76.4%),
specificity (83.33%, 66.67%), positive predictive value (96.88%, 92.86%), negative predictive value
(62.5%, 33.3%) and accuracy of (90%, 75%) respectively. IGRA results had no statistical significant
differences between the studied groups with poor agreement with TST ((κ) = 0.025).
Conclusion: IGRAS test had high sensitivity and specificity in diagnosis of active TB. More studies are
needed to evaluate the effect of anti-TB therapy on IGRA level. |
| first_indexed | 2024-12-13T15:26:35Z |
| format | Article |
| id | doaj.art-b46ab13ad5b54351a7c852c61d492a48 |
| institution | Directory Open Access Journal |
| issn | 2357-0601 2357-061X |
| language | English |
| last_indexed | 2024-12-13T15:26:35Z |
| publishDate | 2014-06-01 |
| publisher | Alexandria University |
| record_format | Article |
| series | Journal of High Institute of Public Health |
| spelling | doaj.art-b46ab13ad5b54351a7c852c61d492a482022-12-21T23:40:19ZengAlexandria UniversityJournal of High Institute of Public Health2357-06012357-061X2014-06-01441334010.21608/JHIPH.2014.20355Interferon Gamma Release Assays (IGRA) in the Diagnosis of Active Pulmonary TuberculosisMona K. EL-Deeb0Hatem A. EL Malwany1Yehia M. Khalil2Saher T. Mourad3Chemical Pathology Department, Medical Research Institute, Alexandria University, EgyptChest Diseases Department, Faculty of Medicine, Alexandria University, EgyptChest Diseases Department, Faculty of Medicine, Alexandria University, EgyptChest Diseases Department, Faculty of Medicine, Alexandria University, EgyptBackground: Tuberculosis is one of the deadliest diseases over the world. Diagnosis of pulmonary TB depends on combination various parameters. IGRA measure T cell release of IFN-γ in response to M.TB antigen. WHO policy statement as on IGRA use in low and middle income countries is not established yet. Objective: The present study aimed to compare between tuberculin skin test (TST) and interferon gamma release assay (IGRA) in the diagnosis of active tuberculosis infection and study the effect of 3 months of first line anti-TB therapy on the positivity of the IGRA test. Methods: 40 Egyptian patients were included in the study, and assigned as two groups; Group I comprised 20 patients with negative sputum for AFB by Ziehl-Neelsen stains with positive sputum culture for M.TB and Group II included 20 patients with positive sputum and Ziehl-Neelsen for AFB before and after 3 months of first line of anti-TB therapy. All patients were subjected to full history taking, clinical examination, X- ray chest, lab investigations, ESR measurements, microbiological tests and ELISA measurement of Quantiferon-TB Gold. Results: Lower significant values were found in group II after treatment than before treatment regarding clinical parameters and 1st and 2nd hours ESR. IGRA test and TST showed sensitivity (91.18%, 76.4%), specificity (83.33%, 66.67%), positive predictive value (96.88%, 92.86%), negative predictive value (62.5%, 33.3%) and accuracy of (90%, 75%) respectively. IGRA results had no statistical significant differences between the studied groups with poor agreement with TST ((κ) = 0.025). Conclusion: IGRAS test had high sensitivity and specificity in diagnosis of active TB. More studies are needed to evaluate the effect of anti-TB therapy on IGRA level.interferon gamma release assays (igra)tuberculosis (tb)tuberculin skin test (tst) |
| spellingShingle | Mona K. EL-Deeb Hatem A. EL Malwany Yehia M. Khalil Saher T. Mourad Interferon Gamma Release Assays (IGRA) in the Diagnosis of Active Pulmonary Tuberculosis Journal of High Institute of Public Health interferon gamma release assays (igra) tuberculosis (tb) tuberculin skin test (tst) |
| title | Interferon Gamma Release Assays (IGRA) in the Diagnosis of Active Pulmonary Tuberculosis |
| title_full | Interferon Gamma Release Assays (IGRA) in the Diagnosis of Active Pulmonary Tuberculosis |
| title_fullStr | Interferon Gamma Release Assays (IGRA) in the Diagnosis of Active Pulmonary Tuberculosis |
| title_full_unstemmed | Interferon Gamma Release Assays (IGRA) in the Diagnosis of Active Pulmonary Tuberculosis |
| title_short | Interferon Gamma Release Assays (IGRA) in the Diagnosis of Active Pulmonary Tuberculosis |
| title_sort | interferon gamma release assays igra in the diagnosis of active pulmonary tuberculosis |
| topic | interferon gamma release assays (igra) tuberculosis (tb) tuberculin skin test (tst) |
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