Extending the Storage Time of <em>Clanis bilineata tsingtauica</em> (Lepidoptera; Sphingidae) Eggs through Variable-Temperature Cold Storage
<i>Clanis bilineata tsingtauica</i> Mell, 1922 (Lepidoptera, Sphingidae), also known as “Doudan” in China, is an important pest in legume crops. As an edible insect, it is most commonly consumed in Jiangsu, Shandong, and Henan Provinces. Mass rearing requires access to large numbers of e...
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| Format: | Article |
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MDPI AG
2021-11-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/10/11/2820 |
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| author | Chenxu Zhu Ming Zhao Haibo Zhang Fang Zhang Yuzhou Du Mingxing Lu |
| author_facet | Chenxu Zhu Ming Zhao Haibo Zhang Fang Zhang Yuzhou Du Mingxing Lu |
| author_sort | Chenxu Zhu |
| collection | DOAJ |
| description | <i>Clanis bilineata tsingtauica</i> Mell, 1922 (Lepidoptera, Sphingidae), also known as “Doudan” in China, is an important pest in legume crops. As an edible insect, it is most commonly consumed in Jiangsu, Shandong, and Henan Provinces. Mass rearing requires access to large numbers of eggs. This stage, however, is of short duration and supplies are frequently not sufficient for insect production. Therefore, we identified the cold storage conditions for <i>C. bilineata tsingtauica</i> that can effectively prolong the storage time of the eggs, to make supplies more readily available. We found that when stored at 4 °C, only 7.5% of the eggs hatched after 7 days, while at 10 °C the hatch rate was 78.3%. At 15 °C, the egg hatch rate remained at this same level (77.8% even after 14–20 days). Considering various combinations, we found that optimal egg hatch occurred if eggs were stored at 15 °C for 11 days, and then held at 15–20 °C under dark conditions. Stored as described above, the egg hatch rate was not significantly different from the control group (at 28 °C). These conditions allow for easier mass rearing of <i>C. bilineata tsingtauica</i> by providing a stable supply of eggs. |
| first_indexed | 2024-03-10T05:29:50Z |
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| id | doaj.art-b46d58bb86e5458bad5758f1da44a250 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T05:29:50Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
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| series | Foods |
| spelling | doaj.art-b46d58bb86e5458bad5758f1da44a2502023-11-22T23:22:31ZengMDPI AGFoods2304-81582021-11-011011282010.3390/foods10112820Extending the Storage Time of <em>Clanis bilineata tsingtauica</em> (Lepidoptera; Sphingidae) Eggs through Variable-Temperature Cold StorageChenxu Zhu0Ming Zhao1Haibo Zhang2Fang Zhang3Yuzhou Du4Mingxing Lu5College of Horticulture and Plant Protection & Institute of Applied Entomology, Yangzhou University, Yangzhou 225009, ChinaCollege of Horticulture and Plant Protection & Institute of Applied Entomology, Yangzhou University, Yangzhou 225009, ChinaPlant Protection and Quarantine Station of Jiangsu Province, Nanjing 210009, ChinaPlant Protection and Quarantine Station of Jiangsu Province, Nanjing 210009, ChinaCollege of Horticulture and Plant Protection & Institute of Applied Entomology, Yangzhou University, Yangzhou 225009, ChinaCollege of Horticulture and Plant Protection & Institute of Applied Entomology, Yangzhou University, Yangzhou 225009, China<i>Clanis bilineata tsingtauica</i> Mell, 1922 (Lepidoptera, Sphingidae), also known as “Doudan” in China, is an important pest in legume crops. As an edible insect, it is most commonly consumed in Jiangsu, Shandong, and Henan Provinces. Mass rearing requires access to large numbers of eggs. This stage, however, is of short duration and supplies are frequently not sufficient for insect production. Therefore, we identified the cold storage conditions for <i>C. bilineata tsingtauica</i> that can effectively prolong the storage time of the eggs, to make supplies more readily available. We found that when stored at 4 °C, only 7.5% of the eggs hatched after 7 days, while at 10 °C the hatch rate was 78.3%. At 15 °C, the egg hatch rate remained at this same level (77.8% even after 14–20 days). Considering various combinations, we found that optimal egg hatch occurred if eggs were stored at 15 °C for 11 days, and then held at 15–20 °C under dark conditions. Stored as described above, the egg hatch rate was not significantly different from the control group (at 28 °C). These conditions allow for easier mass rearing of <i>C. bilineata tsingtauica</i> by providing a stable supply of eggs.https://www.mdpi.com/2304-8158/10/11/2820<i>Clanis bilineata tsingtauica</i>edible insectscold storagetemperatureartificial breeding |
| spellingShingle | Chenxu Zhu Ming Zhao Haibo Zhang Fang Zhang Yuzhou Du Mingxing Lu Extending the Storage Time of <em>Clanis bilineata tsingtauica</em> (Lepidoptera; Sphingidae) Eggs through Variable-Temperature Cold Storage Foods <i>Clanis bilineata tsingtauica</i> edible insects cold storage temperature artificial breeding |
| title | Extending the Storage Time of <em>Clanis bilineata tsingtauica</em> (Lepidoptera; Sphingidae) Eggs through Variable-Temperature Cold Storage |
| title_full | Extending the Storage Time of <em>Clanis bilineata tsingtauica</em> (Lepidoptera; Sphingidae) Eggs through Variable-Temperature Cold Storage |
| title_fullStr | Extending the Storage Time of <em>Clanis bilineata tsingtauica</em> (Lepidoptera; Sphingidae) Eggs through Variable-Temperature Cold Storage |
| title_full_unstemmed | Extending the Storage Time of <em>Clanis bilineata tsingtauica</em> (Lepidoptera; Sphingidae) Eggs through Variable-Temperature Cold Storage |
| title_short | Extending the Storage Time of <em>Clanis bilineata tsingtauica</em> (Lepidoptera; Sphingidae) Eggs through Variable-Temperature Cold Storage |
| title_sort | extending the storage time of em clanis bilineata tsingtauica em lepidoptera sphingidae eggs through variable temperature cold storage |
| topic | <i>Clanis bilineata tsingtauica</i> edible insects cold storage temperature artificial breeding |
| url | https://www.mdpi.com/2304-8158/10/11/2820 |
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