Myristoylation profiling in human cells and zebrafish
Human cells (HEK 293, HeLa, MCF-7) and zebrafish embryos were metabolically tagged with an alkynyl myristic acid probe, lysed with an SDS buffer and tagged proteomes ligated to multifunctional capture reagents via copper-catalyzed alkyne azide cycloaddition (CuAAC). This allowed for affinity enrichm...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2015-09-01
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| Series: | Data in Brief |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352340915001055 |
| _version_ | 1818289439289376768 |
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| author | Malgorzata Broncel Remigiusz A Serwa Paulina Ciepla Eberhard Krause Margaret J. Dallman Anthony I. Magee Edward W. Tate |
| author_facet | Malgorzata Broncel Remigiusz A Serwa Paulina Ciepla Eberhard Krause Margaret J. Dallman Anthony I. Magee Edward W. Tate |
| author_sort | Malgorzata Broncel |
| collection | DOAJ |
| description | Human cells (HEK 293, HeLa, MCF-7) and zebrafish embryos were metabolically tagged with an alkynyl myristic acid probe, lysed with an SDS buffer and tagged proteomes ligated to multifunctional capture reagents via copper-catalyzed alkyne azide cycloaddition (CuAAC). This allowed for affinity enrichment and high-confidence identification, by delivering direct MS/MS evidence for the modification site, of 87 and 61 co-translationally myristoylated proteins in human cells and zebrafish, respectively. The data have been deposited to ProteomeXchange Consortium (Vizcaíno et al., 2014 Nat. Biotechnol., 32, 223–6) (PXD001863 and PXD001876) and are described in detail in Multifunctional reagents for quantitative proteome-wide analysis of protein modification in human cells and dynamic protein lipidation during vertebrate development׳ by Broncel et al., Angew. Chem. Int. Ed. |
| first_indexed | 2024-12-13T02:12:18Z |
| format | Article |
| id | doaj.art-b46e0e25d4cc47ae80460e01c7a10398 |
| institution | Directory Open Access Journal |
| issn | 2352-3409 |
| language | English |
| last_indexed | 2024-12-13T02:12:18Z |
| publishDate | 2015-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Data in Brief |
| spelling | doaj.art-b46e0e25d4cc47ae80460e01c7a103982022-12-22T00:03:00ZengElsevierData in Brief2352-34092015-09-014C37938310.1016/j.dib.2015.06.010Myristoylation profiling in human cells and zebrafishMalgorzata Broncel0Remigiusz A Serwa1Paulina Ciepla2Eberhard Krause3Margaret J. Dallman4Anthony I. Magee5Edward W. Tate6Department of Chemistry, Imperial College London, Exhibition Road, London SW7 2AZ, UKDepartment of Chemistry, Imperial College London, Exhibition Road, London SW7 2AZ, UKDepartment of Chemistry, Imperial College London, Exhibition Road, London SW7 2AZ, UKLeibniz-Institut für Molekulare Pharmakologie (FMP), Robert-Roessle-Str. 10, 13125 Berlin, GermanyDepartment of Life Sciences, Imperial College London, Exhibition Road, London SW7 2AZ, UKNational Heart and Lung Institute, Imperial College London, Exhibition Road, London SW7 2AZ, UKDepartment of Chemistry, Imperial College London, Exhibition Road, London SW7 2AZ, UKHuman cells (HEK 293, HeLa, MCF-7) and zebrafish embryos were metabolically tagged with an alkynyl myristic acid probe, lysed with an SDS buffer and tagged proteomes ligated to multifunctional capture reagents via copper-catalyzed alkyne azide cycloaddition (CuAAC). This allowed for affinity enrichment and high-confidence identification, by delivering direct MS/MS evidence for the modification site, of 87 and 61 co-translationally myristoylated proteins in human cells and zebrafish, respectively. The data have been deposited to ProteomeXchange Consortium (Vizcaíno et al., 2014 Nat. Biotechnol., 32, 223–6) (PXD001863 and PXD001876) and are described in detail in Multifunctional reagents for quantitative proteome-wide analysis of protein modification in human cells and dynamic protein lipidation during vertebrate development׳ by Broncel et al., Angew. Chem. Int. Ed.http://www.sciencedirect.com/science/article/pii/S2352340915001055Protein modificationMyristoylationTagging by substrateModification site |
| spellingShingle | Malgorzata Broncel Remigiusz A Serwa Paulina Ciepla Eberhard Krause Margaret J. Dallman Anthony I. Magee Edward W. Tate Myristoylation profiling in human cells and zebrafish Data in Brief Protein modification Myristoylation Tagging by substrate Modification site |
| title | Myristoylation profiling in human cells and zebrafish |
| title_full | Myristoylation profiling in human cells and zebrafish |
| title_fullStr | Myristoylation profiling in human cells and zebrafish |
| title_full_unstemmed | Myristoylation profiling in human cells and zebrafish |
| title_short | Myristoylation profiling in human cells and zebrafish |
| title_sort | myristoylation profiling in human cells and zebrafish |
| topic | Protein modification Myristoylation Tagging by substrate Modification site |
| url | http://www.sciencedirect.com/science/article/pii/S2352340915001055 |
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