Myristoylation profiling in human cells and zebrafish

Myristoylation profiling in human cells and zebrafish

Human cells (HEK 293, HeLa, MCF-7) and zebrafish embryos were metabolically tagged with an alkynyl myristic acid probe, lysed with an SDS buffer and tagged proteomes ligated to multifunctional capture reagents via copper-catalyzed alkyne azide cycloaddition (CuAAC). This allowed for affinity enrichm...

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Bibliographic Details
Main Authors: Malgorzata Broncel, Remigiusz A Serwa, Paulina Ciepla, Eberhard Krause, Margaret J. Dallman, Anthony I. Magee, Edward W. Tate
Format: Article
Language:English
Published: Elsevier 2015-09-01
Series:Data in Brief
Subjects:
Protein modification
Myristoylation
Tagging by substrate
Modification site
Online Access:http://www.sciencedirect.com/science/article/pii/S2352340915001055

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