Changes of the gut microbiota composition and short chain fatty acid in patients with atrial fibrillation
Background With the establishment of the cardiac-gut axis concept, increasing evidence has suggested the involvement and important regulatory role of the gut microbiota (GM) and short chain fatty acid (SCFA) in cardiovascular diseases. However, the relationship between GM and atrial fibrillation (AF...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
PeerJ Inc.
2023-12-01
|
| Series: | PeerJ |
| Subjects: | |
| Online Access: | https://peerj.com/articles/16228.pdf |
| _version_ | 1797398854524469248 |
|---|---|
| author | Lingzhi Chen Jinxin Chen Yuheng Huang Yanran Wu Junfeng Li Weicheng Ni Yucheng Lu Zhenzhen Li Chuhuan Zhao Shuting Kong Hao Zhou Xiang Qu |
| author_facet | Lingzhi Chen Jinxin Chen Yuheng Huang Yanran Wu Junfeng Li Weicheng Ni Yucheng Lu Zhenzhen Li Chuhuan Zhao Shuting Kong Hao Zhou Xiang Qu |
| author_sort | Lingzhi Chen |
| collection | DOAJ |
| description | Background With the establishment of the cardiac-gut axis concept, increasing evidence has suggested the involvement and important regulatory role of the gut microbiota (GM) and short chain fatty acid (SCFA) in cardiovascular diseases. However, the relationship between GM and atrial fibrillation (AF) is still poorly understood. Objectives The aim of this study was to investigate whether there were differences in GM and SCFA between AF patients and healthy controls. Methods In this study, we enrolled 30 hospitalized patients with AF and 30 matched patients with sinus rhythm (SR). GM species in fecal samples were evaluated through amplicon sequencing targeting the 16Sribosomal RNA gene. The feces SCFAs were describe step by step the quantitative analysis using gas chromatography-mass spectrometry (GC-MS). GM species richness, diversity, differential abundance of individual taxa between AF and SR were analyzed. Results AF patients showed decreased species richness and α-diversity compared to SR patients, but there was no statistical difference. The phylogenetic diversity was significant decreased in AF group. The β-diversity indexes revealed significant differences in GM community structure between the AF group and the SR group. After investigated the individual taxa, AF group showed altered relative abundance in several taxa compared to the SR group. linear discriminant analysis (LDA) effect size (LEfSe) analysis revealed, a significant decrease in Bifidobacterium and a greater abundance of Lactobacillus, Fusobacterium, Haemophilus in AF group compared with the SR group. The abundance of haemophilus was negative correlated with isovaleric acid and isobutyric acid. Conclusions In AF patients, the GM phylogenetic diversity and β-diversity decreased, the relative abundance altered in several taxa and the bacterial community structure changed as well as the SCFA level. GM and SCFA dysbiosis might play a crucial part in the occurrence and development of AF. |
| first_indexed | 2024-03-09T01:31:35Z |
| format | Article |
| id | doaj.art-b4723c06ee4a41f2804bc3eb7ebf20cc |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T01:31:35Z |
| publishDate | 2023-12-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-b4723c06ee4a41f2804bc3eb7ebf20cc2023-12-09T15:05:04ZengPeerJ Inc.PeerJ2167-83592023-12-0111e1622810.7717/peerj.16228Changes of the gut microbiota composition and short chain fatty acid in patients with atrial fibrillationLingzhi Chen0Jinxin Chen1Yuheng Huang2Yanran Wu3Junfeng Li4Weicheng Ni5Yucheng Lu6Zhenzhen Li7Chuhuan Zhao8Shuting Kong9Hao Zhou10Xiang Qu11Wenzhou Central Hospital, Wenzhou, ChinaThe First Affiliated Hospital of Wenzhou Medical University, Wenzhou, ChinaThe First Affiliated Hospital of Wenzhou Medical University, Wenzhou, ChinaThe First Affiliated Hospital of Wenzhou Medical University, Wenzhou, ChinaThe First Affiliated Hospital of Wenzhou Medical University, Wenzhou, ChinaThe First Affiliated Hospital of Wenzhou Medical University, Wenzhou, ChinaThe First Affiliated Hospital of Wenzhou Medical University, Wenzhou, ChinaThe First Affiliated Hospital of Wenzhou Medical University, Wenzhou, ChinaThe First Affiliated Hospital of Wenzhou Medical University, Wenzhou, ChinaThe First Affiliated Hospital of Wenzhou Medical University, Wenzhou, ChinaThe First Affiliated Hospital of Wenzhou Medical University, Wenzhou, ChinaThe First Affiliated Hospital of Wenzhou Medical University, Wenzhou, ChinaBackground With the establishment of the cardiac-gut axis concept, increasing evidence has suggested the involvement and important regulatory role of the gut microbiota (GM) and short chain fatty acid (SCFA) in cardiovascular diseases. However, the relationship between GM and atrial fibrillation (AF) is still poorly understood. Objectives The aim of this study was to investigate whether there were differences in GM and SCFA between AF patients and healthy controls. Methods In this study, we enrolled 30 hospitalized patients with AF and 30 matched patients with sinus rhythm (SR). GM species in fecal samples were evaluated through amplicon sequencing targeting the 16Sribosomal RNA gene. The feces SCFAs were describe step by step the quantitative analysis using gas chromatography-mass spectrometry (GC-MS). GM species richness, diversity, differential abundance of individual taxa between AF and SR were analyzed. Results AF patients showed decreased species richness and α-diversity compared to SR patients, but there was no statistical difference. The phylogenetic diversity was significant decreased in AF group. The β-diversity indexes revealed significant differences in GM community structure between the AF group and the SR group. After investigated the individual taxa, AF group showed altered relative abundance in several taxa compared to the SR group. linear discriminant analysis (LDA) effect size (LEfSe) analysis revealed, a significant decrease in Bifidobacterium and a greater abundance of Lactobacillus, Fusobacterium, Haemophilus in AF group compared with the SR group. The abundance of haemophilus was negative correlated with isovaleric acid and isobutyric acid. Conclusions In AF patients, the GM phylogenetic diversity and β-diversity decreased, the relative abundance altered in several taxa and the bacterial community structure changed as well as the SCFA level. GM and SCFA dysbiosis might play a crucial part in the occurrence and development of AF.https://peerj.com/articles/16228.pdfGut microbiotaAtrial fibrillationShort chain fatty acid |
| spellingShingle | Lingzhi Chen Jinxin Chen Yuheng Huang Yanran Wu Junfeng Li Weicheng Ni Yucheng Lu Zhenzhen Li Chuhuan Zhao Shuting Kong Hao Zhou Xiang Qu Changes of the gut microbiota composition and short chain fatty acid in patients with atrial fibrillation PeerJ Gut microbiota Atrial fibrillation Short chain fatty acid |
| title | Changes of the gut microbiota composition and short chain fatty acid in patients with atrial fibrillation |
| title_full | Changes of the gut microbiota composition and short chain fatty acid in patients with atrial fibrillation |
| title_fullStr | Changes of the gut microbiota composition and short chain fatty acid in patients with atrial fibrillation |
| title_full_unstemmed | Changes of the gut microbiota composition and short chain fatty acid in patients with atrial fibrillation |
| title_short | Changes of the gut microbiota composition and short chain fatty acid in patients with atrial fibrillation |
| title_sort | changes of the gut microbiota composition and short chain fatty acid in patients with atrial fibrillation |
| topic | Gut microbiota Atrial fibrillation Short chain fatty acid |
| url | https://peerj.com/articles/16228.pdf |
| work_keys_str_mv | AT lingzhichen changesofthegutmicrobiotacompositionandshortchainfattyacidinpatientswithatrialfibrillation AT jinxinchen changesofthegutmicrobiotacompositionandshortchainfattyacidinpatientswithatrialfibrillation AT yuhenghuang changesofthegutmicrobiotacompositionandshortchainfattyacidinpatientswithatrialfibrillation AT yanranwu changesofthegutmicrobiotacompositionandshortchainfattyacidinpatientswithatrialfibrillation AT junfengli changesofthegutmicrobiotacompositionandshortchainfattyacidinpatientswithatrialfibrillation AT weichengni changesofthegutmicrobiotacompositionandshortchainfattyacidinpatientswithatrialfibrillation AT yuchenglu changesofthegutmicrobiotacompositionandshortchainfattyacidinpatientswithatrialfibrillation AT zhenzhenli changesofthegutmicrobiotacompositionandshortchainfattyacidinpatientswithatrialfibrillation AT chuhuanzhao changesofthegutmicrobiotacompositionandshortchainfattyacidinpatientswithatrialfibrillation AT shutingkong changesofthegutmicrobiotacompositionandshortchainfattyacidinpatientswithatrialfibrillation AT haozhou changesofthegutmicrobiotacompositionandshortchainfattyacidinpatientswithatrialfibrillation AT xiangqu changesofthegutmicrobiotacompositionandshortchainfattyacidinpatientswithatrialfibrillation |