Antibacterial Activities of Oregano- N-acetyl cysteine Nanocomposite against Multidrug-resistant Riemerella anatipestifer Isolated from Ducks
Riemerella anatipestifer (R. anatipestifer) considered a highly epizootic pathogen, causes (duck septicemia) resulting in significant ducklings mortality and surviving birds may grow poorly leading to significant worldwide economic losses in the duck industry. As emerging of extensive multidrug res...
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| Format: | Article |
| Language: | English |
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Assiut University
2023-08-01
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| Series: | Journal of Advanced Veterinary Research |
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| Online Access: | https://advetresearch.com/index.php/AVR/article/view/1433 |
| _version_ | 1797657140516618240 |
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| author | Samah Eid Yousreya Hashem Nayera M. Al-Atfeeh Heba A. Baz Abeer Mwafy Dalia M.A. Elmasry |
| author_facet | Samah Eid Yousreya Hashem Nayera M. Al-Atfeeh Heba A. Baz Abeer Mwafy Dalia M.A. Elmasry |
| author_sort | Samah Eid |
| collection | DOAJ |
| description |
Riemerella anatipestifer (R. anatipestifer) considered a highly epizootic pathogen, causes (duck septicemia) resulting in significant ducklings mortality and surviving birds may grow poorly leading to significant worldwide economic losses in the duck industry. As emerging of extensive multidrug resistance strains of R. anatipestifer, alternative treatment is of great concern for R. anatipestifer control. During (January - August 2020), different outbreaks in 20 suspected field duck farms, and 6 R. anatipestifer isolates which were confirmed by conventional PCR technique underwent an in vitro study. The isolates were tested against 17 antimicrobial agents (widely used in the poultry industry) to assess their antimicrobial resistance resulting that all tested strains being multidrug-resistant. The obtained antimicrobial resistance index (AMRI) was 0.63 ± 0.07, a very high value. So, the alternative treatment seemed to be a valuable tool for R. anatipestifer control. Oregano nanoemulsion and its nanocomposite were characterized by TEM and the nano-size were 23.46 and 37.13 nm, respectively, and with a stable state, zeta potentials were 18.5 ± 5.11, 10.031 ± 5.11, the PDI were 0.236, 0.467 and IC50 is > 100 µg/ml and IC50 is 28.13 µg/ml, respectively). The study concluded that the tested R. anatipestifer strains are extensively multidrug-resistant and its control requires an alternative interference other than the antibiotics. Oregano- N-acetyl cysteine nanocomposite shows promising high activity against R. anatipestifer, which to be recommended.
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| first_indexed | 2024-03-11T17:40:04Z |
| format | Article |
| id | doaj.art-b4726b7defd7427d809f9460d6510582 |
| institution | Directory Open Access Journal |
| issn | 2090-6269 2090-6277 |
| language | English |
| last_indexed | 2024-03-11T17:40:04Z |
| publishDate | 2023-08-01 |
| publisher | Assiut University |
| record_format | Article |
| series | Journal of Advanced Veterinary Research |
| spelling | doaj.art-b4726b7defd7427d809f9460d65105822023-10-18T14:10:06ZengAssiut UniversityJournal of Advanced Veterinary Research2090-62692090-62772023-08-01137Antibacterial Activities of Oregano- N-acetyl cysteine Nanocomposite against Multidrug-resistant Riemerella anatipestifer Isolated from DucksSamah Eid0Yousreya Hashem1Nayera M. Al-Atfeeh2Heba A. Baz3Abeer Mwafy4Dalia M.A. Elmasry5Reference Laboratory for Veterinary Quality Control on Poultry Production (RLQP), Animal Health Research Institute (AHRI), Agriculture Research Center (ARC), P.O. Box 264-Dokki, Nadi El-Seid St., Giza 12618, Egypt.Mycoplasma Research Department, Animal Health Research Institute, Agriculture Research Center (ARC), Giza 12618, Egypt. Reference Laboratory for Veterinary Quality Control on Poultry Production (RLQP), Animal Health Research Institute (AHRI), Agriculture Research Center (ARC), P.O. Box 264-Dokki, Nadi El-Seid St., Giza 12618, Egypt.Educational Veterinary Hospital, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt.Department of Microbiology, Faculty of Veterinary medicine, New Valley University, Egypt.Nanomaterial Research and synthesis Unit, Animal Health Research Institute (AHRI), Agriculture Research Center (ARC), P.O. Box 264-Dokki, Nadi El-Seid St., Giza 12618, Egypt. Riemerella anatipestifer (R. anatipestifer) considered a highly epizootic pathogen, causes (duck septicemia) resulting in significant ducklings mortality and surviving birds may grow poorly leading to significant worldwide economic losses in the duck industry. As emerging of extensive multidrug resistance strains of R. anatipestifer, alternative treatment is of great concern for R. anatipestifer control. During (January - August 2020), different outbreaks in 20 suspected field duck farms, and 6 R. anatipestifer isolates which were confirmed by conventional PCR technique underwent an in vitro study. The isolates were tested against 17 antimicrobial agents (widely used in the poultry industry) to assess their antimicrobial resistance resulting that all tested strains being multidrug-resistant. The obtained antimicrobial resistance index (AMRI) was 0.63 ± 0.07, a very high value. So, the alternative treatment seemed to be a valuable tool for R. anatipestifer control. Oregano nanoemulsion and its nanocomposite were characterized by TEM and the nano-size were 23.46 and 37.13 nm, respectively, and with a stable state, zeta potentials were 18.5 ± 5.11, 10.031 ± 5.11, the PDI were 0.236, 0.467 and IC50 is > 100 µg/ml and IC50 is 28.13 µg/ml, respectively). The study concluded that the tested R. anatipestifer strains are extensively multidrug-resistant and its control requires an alternative interference other than the antibiotics. Oregano- N-acetyl cysteine nanocomposite shows promising high activity against R. anatipestifer, which to be recommended. https://advetresearch.com/index.php/AVR/article/view/1433 R. anatipestiferAMRIOregano- N-acetyl cysteine Nanoalternative medicine |
| spellingShingle | Samah Eid Yousreya Hashem Nayera M. Al-Atfeeh Heba A. Baz Abeer Mwafy Dalia M.A. Elmasry Antibacterial Activities of Oregano- N-acetyl cysteine Nanocomposite against Multidrug-resistant Riemerella anatipestifer Isolated from Ducks Journal of Advanced Veterinary Research R. anatipestifer AMRI Oregano- N-acetyl cysteine Nanoalternative medicine |
| title | Antibacterial Activities of Oregano- N-acetyl cysteine Nanocomposite against Multidrug-resistant Riemerella anatipestifer Isolated from Ducks |
| title_full | Antibacterial Activities of Oregano- N-acetyl cysteine Nanocomposite against Multidrug-resistant Riemerella anatipestifer Isolated from Ducks |
| title_fullStr | Antibacterial Activities of Oregano- N-acetyl cysteine Nanocomposite against Multidrug-resistant Riemerella anatipestifer Isolated from Ducks |
| title_full_unstemmed | Antibacterial Activities of Oregano- N-acetyl cysteine Nanocomposite against Multidrug-resistant Riemerella anatipestifer Isolated from Ducks |
| title_short | Antibacterial Activities of Oregano- N-acetyl cysteine Nanocomposite against Multidrug-resistant Riemerella anatipestifer Isolated from Ducks |
| title_sort | antibacterial activities of oregano n acetyl cysteine nanocomposite against multidrug resistant riemerella anatipestifer isolated from ducks |
| topic | R. anatipestifer AMRI Oregano- N-acetyl cysteine Nanoalternative medicine |
| url | https://advetresearch.com/index.php/AVR/article/view/1433 |
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