Yeast Mitochondrial Translation Initiation Factor 3 Interacts with Pet111p to Promote <i>COX2</i> mRNA Translation
Mitochondrial genomes code for several core components of respiratory chain complexes. Thus, mitochondrial translation is of great importance for the organelle as well as for the whole cell. In yeast, mitochondrial translation initiation factor 3, Aim23p, is not essential for the organellar protein...
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MDPI AG
2020-05-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/10/3414 |
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| author | Ivan Chicherin Sergey Levitskii Maria V. Baleva Igor A. Krasheninnikov Maxim V. Patrushev Piotr Kamenski |
| author_facet | Ivan Chicherin Sergey Levitskii Maria V. Baleva Igor A. Krasheninnikov Maxim V. Patrushev Piotr Kamenski |
| author_sort | Ivan Chicherin |
| collection | DOAJ |
| description | Mitochondrial genomes code for several core components of respiratory chain complexes. Thus, mitochondrial translation is of great importance for the organelle as well as for the whole cell. In yeast, mitochondrial translation initiation factor 3, Aim23p, is not essential for the organellar protein synthesis; however, its absence leads to a significant quantitative imbalance of the mitochondrial translation products. This fact points to a possible specific action of Aim23p on the biosynthesis of some mitochondrial protein species. In this work, we examined such peculiar effects of Aim23p in relation to yeast mitochondrial <i>COX2</i> mRNA translation. We show that Aim23p is indispensable to this process. According to our data, this is mediated by Aimp23p interaction with the known specific factor of the <i>COX2</i> mRNA translation, Pet111p. If there is no Aim23p in the yeast cells, an increased amount of Pet111p ensures proper <i>COX2</i> mRNA translation. Our results demonstrate the additional non-canonical function of initiation factor 3 in yeast mitochondrial translation. |
| first_indexed | 2024-03-10T19:53:50Z |
| format | Article |
| id | doaj.art-b474463fc37542539f171c0acf6426ac |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T19:53:50Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-b474463fc37542539f171c0acf6426ac2023-11-20T00:09:58ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-05-012110341410.3390/ijms21103414Yeast Mitochondrial Translation Initiation Factor 3 Interacts with Pet111p to Promote <i>COX2</i> mRNA TranslationIvan Chicherin0Sergey Levitskii1Maria V. Baleva2Igor A. Krasheninnikov3Maxim V. Patrushev4Piotr Kamenski5Faculty of Biology, M.V.Lomonosov Moscow State University, Moscow 119234, RussiaFaculty of Biology, M.V.Lomonosov Moscow State University, Moscow 119234, RussiaFaculty of Biology, M.V.Lomonosov Moscow State University, Moscow 119234, RussiaFaculty of Biology, M.V.Lomonosov Moscow State University, Moscow 119234, RussiaNational Research Centre “Kurchatov Institute”, 1 Akademik Kurchatov Square, 123182 Moscow, RussiaFaculty of Biology, M.V.Lomonosov Moscow State University, Moscow 119234, RussiaMitochondrial genomes code for several core components of respiratory chain complexes. Thus, mitochondrial translation is of great importance for the organelle as well as for the whole cell. In yeast, mitochondrial translation initiation factor 3, Aim23p, is not essential for the organellar protein synthesis; however, its absence leads to a significant quantitative imbalance of the mitochondrial translation products. This fact points to a possible specific action of Aim23p on the biosynthesis of some mitochondrial protein species. In this work, we examined such peculiar effects of Aim23p in relation to yeast mitochondrial <i>COX2</i> mRNA translation. We show that Aim23p is indispensable to this process. According to our data, this is mediated by Aimp23p interaction with the known specific factor of the <i>COX2</i> mRNA translation, Pet111p. If there is no Aim23p in the yeast cells, an increased amount of Pet111p ensures proper <i>COX2</i> mRNA translation. Our results demonstrate the additional non-canonical function of initiation factor 3 in yeast mitochondrial translation.https://www.mdpi.com/1422-0067/21/10/3414mitochondriatranslationinitiation factortranslational activator |
| spellingShingle | Ivan Chicherin Sergey Levitskii Maria V. Baleva Igor A. Krasheninnikov Maxim V. Patrushev Piotr Kamenski Yeast Mitochondrial Translation Initiation Factor 3 Interacts with Pet111p to Promote <i>COX2</i> mRNA Translation International Journal of Molecular Sciences mitochondria translation initiation factor translational activator |
| title | Yeast Mitochondrial Translation Initiation Factor 3 Interacts with Pet111p to Promote <i>COX2</i> mRNA Translation |
| title_full | Yeast Mitochondrial Translation Initiation Factor 3 Interacts with Pet111p to Promote <i>COX2</i> mRNA Translation |
| title_fullStr | Yeast Mitochondrial Translation Initiation Factor 3 Interacts with Pet111p to Promote <i>COX2</i> mRNA Translation |
| title_full_unstemmed | Yeast Mitochondrial Translation Initiation Factor 3 Interacts with Pet111p to Promote <i>COX2</i> mRNA Translation |
| title_short | Yeast Mitochondrial Translation Initiation Factor 3 Interacts with Pet111p to Promote <i>COX2</i> mRNA Translation |
| title_sort | yeast mitochondrial translation initiation factor 3 interacts with pet111p to promote i cox2 i mrna translation |
| topic | mitochondria translation initiation factor translational activator |
| url | https://www.mdpi.com/1422-0067/21/10/3414 |
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