Meningkatkan produksi flavan-3-ol melalui kalus Camellia sinensis L. dengan elisator Cu2+

Flavan-3-ol is a secondary metabolite in tea plant that is used as anti obesity agent. The diffi culty in obtaining Flavan-3-ol outof tea plant is due to dependency on season, need for large land, need for very intensive care and relatively low production. Therefore fl avan 3-ol production needs tob...

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Main Authors: B Sutini, W Tatik, W Wahyu, Sutiman B. Sumitro
Format: Article
Language:English
Published: Indonesian Biological Society 2012-02-01
Series:Journal of Biological Researches
Subjects:
Online Access:http://berkalahayati.org/journal/7d63b162
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author B Sutini
W Tatik
W Wahyu
Sutiman B. Sumitro
author_facet B Sutini
W Tatik
W Wahyu
Sutiman B. Sumitro
author_sort B Sutini
collection DOAJ
description Flavan-3-ol is a secondary metabolite in tea plant that is used as anti obesity agent. The diffi culty in obtaining Flavan-3-ol outof tea plant is due to dependency on season, need for large land, need for very intensive care and relatively low production. Therefore fl avan 3-ol production needs tobe developed in vitro culture technique. This technique can cope with the handicapes above. It caneffectively control production and requires less space. The purpose of this research was to enhance fl avan 3-ol production by modifyingmedium and precursor appropriately. The steps of this process were: (1) Callus induction through cultivating the tea shoot explants onmedium fi lled with some growth hormone, (2) fl avan-3-ol induction on callus culture using Cu 2+ elicitor. (3) Observation over callusgrowth, (4) Observation over qualitative characterizes of fl avan 3-ol. The result of the research using Cu 2+ elicitor shows that theproduction of fl avan-3-ol increases by 12.5%.
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spelling doaj.art-b4754c6b43354f5ca0a6f163fd9af4952022-12-21T21:03:29ZengIndonesian Biological SocietyJournal of Biological Researches0852-68342337-389X2012-02-01141394410.23869/bphjbr.14.1.20086Meningkatkan produksi flavan-3-ol melalui kalus Camellia sinensis L. dengan elisator Cu2+B Sutini0W Tatik1W Wahyu2Sutiman B. Sumitro3Agronomy Department of Agriculture Faculty UPN 'Veteran', Surabaya-East JavaAgronomy Department of Agriculture Faculty UPN 'Veteran', Surabaya-East JavaBiology Department of FMIPA, Brawijaya University, Malang-East JavaBiology Department of FMIPA, Brawijaya University, Malang-East JavaFlavan-3-ol is a secondary metabolite in tea plant that is used as anti obesity agent. The diffi culty in obtaining Flavan-3-ol outof tea plant is due to dependency on season, need for large land, need for very intensive care and relatively low production. Therefore fl avan 3-ol production needs tobe developed in vitro culture technique. This technique can cope with the handicapes above. It caneffectively control production and requires less space. The purpose of this research was to enhance fl avan 3-ol production by modifyingmedium and precursor appropriately. The steps of this process were: (1) Callus induction through cultivating the tea shoot explants onmedium fi lled with some growth hormone, (2) fl avan-3-ol induction on callus culture using Cu 2+ elicitor. (3) Observation over callusgrowth, (4) Observation over qualitative characterizes of fl avan 3-ol. The result of the research using Cu 2+ elicitor shows that theproduction of fl avan-3-ol increases by 12.5%.http://berkalahayati.org/journal/7d63b162Flavan-3-olCallus cultureCamellia sinensis LIon Cu 2+ elicitor
spellingShingle B Sutini
W Tatik
W Wahyu
Sutiman B. Sumitro
Meningkatkan produksi flavan-3-ol melalui kalus Camellia sinensis L. dengan elisator Cu2+
Journal of Biological Researches
Flavan-3-ol
Callus culture
Camellia sinensis L
Ion Cu 2+ elicitor
title Meningkatkan produksi flavan-3-ol melalui kalus Camellia sinensis L. dengan elisator Cu2+
title_full Meningkatkan produksi flavan-3-ol melalui kalus Camellia sinensis L. dengan elisator Cu2+
title_fullStr Meningkatkan produksi flavan-3-ol melalui kalus Camellia sinensis L. dengan elisator Cu2+
title_full_unstemmed Meningkatkan produksi flavan-3-ol melalui kalus Camellia sinensis L. dengan elisator Cu2+
title_short Meningkatkan produksi flavan-3-ol melalui kalus Camellia sinensis L. dengan elisator Cu2+
title_sort meningkatkan produksi flavan 3 ol melalui kalus camellia sinensis l dengan elisator cu2
topic Flavan-3-ol
Callus culture
Camellia sinensis L
Ion Cu 2+ elicitor
url http://berkalahayati.org/journal/7d63b162
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AT wtatik meningkatkanproduksiflavan3olmelaluikaluscamelliasinensisldenganelisatorcu2
AT wwahyu meningkatkanproduksiflavan3olmelaluikaluscamelliasinensisldenganelisatorcu2
AT sutimanbsumitro meningkatkanproduksiflavan3olmelaluikaluscamelliasinensisldenganelisatorcu2